Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Tyrosine phosphorylation and acetylcholine receptor cluster formation in cultured Xenopus muscle cells.
Baker LP
,
Peng HB
.
???displayArticle.abstract???
Aggregation of the nicotinic acetylcholine receptor (AChR) at sites of nerve-muscle contact is one of the earliest events to occur during the development of the neuromuscular junction. The stimulus presented to the muscle by nerve and the mechanisms underlying postsynaptic differentiation are not known. The purpose of this study was to examine the distribution of phosphotyrosine (PY)-containing proteins in cultured Xenopus muscle cells in response to AChR clustering stimuli. Results demonstrated a distinct accumulation of PY at AChR clusters induced by several stimuli, including nerve, the culture substratum, and polystyrene microbeads. AChR microclusters formed by external cross-linking did not show PY colocalization, implying that the accumulation of PY in response to clustering stimuli was not due to the aggregation of basally phosphorylated AChRs. A semi-quantitative determination of the time course for development of PY labeling at bead contacts revealed early PY accumulation within 15 min of contact before significant AChR aggregation. At later stages (within 15 h), the AChR signal came to approximate the PY signal. We have reported the inhibition of bead-induced AChR clustering in response to beads by a tyrphostin tyrosine kinase inhibitor (RG50864) (Peng, H. B., L. P. Baker, and Q. Chen. 1991. Neuron. 6:237-246). RG50864 also inhibited PY accumulation at bead contacts, providing evidence for tyrosine kinase activation in response to the bead stimulus. These results suggest that tyrosine phosphorylation may play an important role in the generative stages of cluster formation, and may involve protein(s) other than or in addition to AChRs.
Anderson,
Nerve-induced remodeling of muscle basal lamina during synaptogenesis.
1986, Pubmed,
Xenbase
Anderson,
Nerve-induced remodeling of muscle basal lamina during synaptogenesis.
1986,
Pubmed
,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Synaptic differentiation can be evoked by polymer microbeads that mimic localized pericellular proteolysis by removing proteins from adjacent surfaces.
1991,
Pubmed
,
Xenbase
Axelrod,
Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.
1980,
Pubmed
Baker,
Induction of acetylcholine receptor clustering by native polystyrene beads. Implication of an endogenous muscle-derived signalling system.
1992,
Pubmed
,
Xenbase
Bloch,
Actin at receptor-rich domains of isolated acetylcholine receptor clusters.
1986,
Pubmed
Bloch,
Molecular events in synaptogenesis: nerve-muscle adhesion and postsynaptic differentiation.
1988,
Pubmed
Bloch,
Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filamin.
1983,
Pubmed
,
Xenbase
Bozyczko,
Integrin on developing and adult skeletal muscle.
1989,
Pubmed
Bridgman,
Distribution of filipin-sterol complexes on cultured muscle cells: cell-substratum contact areas associated with acetylcholine receptor clusters.
1983,
Pubmed
,
Xenbase
Burridge,
Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
1988,
Pubmed
Carlson,
Nerve terminal anchorage protein 1 (TAP-1) is a chondroitin sulfate proteoglycan: biochemical and electron microscopic characterization.
1987,
Pubmed
Changeux,
Acetylcholine receptor: an allosteric protein.
1984,
Pubmed
Connolly,
Role of the cytoskeleton in the formation, stabilization, and removal of acetylcholine receptor clusters in cultured muscle cells.
1984,
Pubmed
Davis,
Presence of an SH2 domain in the actin-binding protein tensin.
1991,
Pubmed
DeClue,
Phosphorylation of talin at tyrosine in Rous sarcoma virus-transformed cells.
1987,
Pubmed
Ferns,
How many agrins does it take to make a synapse?
1992,
Pubmed
Fertuck,
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
1976,
Pubmed
Froehner,
The submembrane machinery for nicotinic acetylcholine receptor clustering.
1991,
Pubmed
Glenney,
Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton.
1989,
Pubmed
Hall,
Cytoplasmic actin in postsynaptic structures at the neuromuscular junction.
1981,
Pubmed
Hirst,
Phosphorylation of the fibronectin receptor complex in cells transformed by oncogenes that encode tyrosine kinases.
1986,
Pubmed
Huganir,
cAMP-dependent protein kinase phosphorylates the nicotinic acetylcholine receptor.
1983,
Pubmed
Huganir,
Regulation of neurotransmitter receptor desensitization by protein phosphorylation.
1990,
Pubmed
Huganir,
Phosphorylation of the nicotinic acetylcholine receptor by an endogenous tyrosine-specific protein kinase.
1984,
Pubmed
Ito,
Vinculin phosphorylation by the src kinase. Interaction of vinculin with phospholipid vesicles.
1983,
Pubmed
Luther,
Membrane-related specializations associated with acetylcholine receptor aggregates induced by electric fields.
1985,
Pubmed
,
Xenbase
Luther,
Formaldehyde-amine fixatives for immunocytochemistry of cultured Xenopus myocytes.
1989,
Pubmed
,
Xenbase
Maher,
Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells.
1985,
Pubmed
McGuire,
Degradation of underlying extracellular matrix by sensory neurons during neurite outgrowth.
1990,
Pubmed
Miles,
Calcitonin gene-related peptide regulates phosphorylation of the nicotinic acetylcholine receptor in rat myotubes.
1989,
Pubmed
Nastuk,
The putative agrin receptor binds ligand in a calcium-dependent manner and aggregates during agrin-induced acetylcholine receptor clustering.
1991,
Pubmed
Olek,
Direct observation of the rapid aggregation of acetylcholine receptors on identified cultured myotubes after exposure to embryonic brain extract.
1983,
Pubmed
Orida,
Electrophoretic movement and localisation of acetylcholine receptors in the embryonic muscle cell membrane.
1978,
Pubmed
,
Xenbase
Pasquale,
Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus.
1986,
Pubmed
Peng,
Tissue culture of Xenopus neurons and muscle cells as a model for studying synaptic induction.
1991,
Pubmed
,
Xenbase
Peng,
A role of tyrosine phosphorylation in the formation of acetylcholine receptor clusters induced by electric fields in cultured Xenopus muscle cells.
1993,
Pubmed
,
Xenbase
Peng,
Formation of postsynaptic specializations induced by latex beads in cultured muscle cells.
1982,
Pubmed
,
Xenbase
Peng,
Early cytoplasmic specialization at the presumptive acetylcholine receptor cluster: a meshwork of thin filaments.
1984,
Pubmed
,
Xenbase
Peng,
Induction of synaptic development in cultured muscle cells by basic fibroblast growth factor.
1991,
Pubmed
,
Xenbase
Prives,
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
1982,
Pubmed
Qu,
Regulation of tyrosine phosphorylation of the nicotinic acetylcholine receptor at the rat neuromuscular junction.
1990,
Pubmed
Safran,
Phosphorylation of the acetylcholine receptor by protein kinase C and identification of the phosphorylation site within the receptor delta subunit.
1987,
Pubmed
Schroeder,
Phosphorylation sites of the nicotinic acetylcholine receptor. A novel site detected in position delta S362.
1991,
Pubmed
Sealock,
Talin is a post-synaptic component of the rat neuromuscular junction.
1986,
Pubmed
Sefton,
Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
1981,
Pubmed
Stadler,
Synaptic vesicles in electromotoneurones. II. Heterogeneity of populations is expressed in uptake properties; exocytosis and insertion of a core proteoglycan into the extracellular matrix.
1987,
Pubmed
Stollberg,
Acetylcholine receptors and concanavalin A-binding sites on cultured Xenopus muscle cells: electrophoresis, diffusion, and aggregation.
1988,
Pubmed
,
Xenbase
Tank,
Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints.
1982,
Pubmed
Turner,
Paxillin is a major phosphotyrosine-containing protein during embryonic development.
1991,
Pubmed
Turner,
Localization of paxillin, a focal adhesion protein, to smooth muscle dense plaques, and the myotendinous and neuromuscular junctions of skeletal muscle.
1991,
Pubmed
,
Xenbase
Usdin,
Purification and characterization of a polypeptide from chick brain that promotes the accumulation of acetylcholine receptors in chick myotubes.
1986,
Pubmed
Wallace,
Agrin induces phosphorylation of the nicotinic acetylcholine receptor.
1991,
Pubmed
Yarden,
Growth factor receptor tyrosine kinases.
1988,
Pubmed
