Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Bovine chromaffin granule membranes undergo Ca(2+)-regulated exocytosis in frog oocytes.
Scheuner D
,
Logsdon CD
,
Holz RW
.
???displayArticle.abstract???
We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chromaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chromaffin granule membranes were injected into Xenopus laevis oocytes. Exocytosis was detected by the appearance of dopamine-beta-hydroxylase of the chromaffin granule membrane in the oocyteplasma membrane. The appearance of dopamine-beta-hydroxylase on the oocyte surface was strongly Ca(2+)-dependent and was stimulated by coinjection of the chromaffin granule membranes with InsP3 or Ca2+/EGTA buffer (18 microM free Ca2+) or by incubation of the injected oocytes in medium containing the Ca2+ ionophore ionomycin. Similar experiments were performed with a subcellular fraction from cultured chromaffin cells enriched with [3H]norepinephrine-containing chromaffin granules. Because the release of [3H]norepinephrine was strongly correlated with the appearance of dopamine-beta-hydroxylase on the oocyte surface, it is likely that intact chromaffin granules and chromaffin granule membranes undergo exocytosis in the oocyte. Thus, the secretory vesicle membrane without normal vesicle contents is competent to undergo the sequence of events leading to exocytosis. Furthermore, the interchangeability of mammalian and amphibian components suggests substantial biochemical conservation of the regulated exocytotic pathway during the evolutionary progression from amphibians to mammals.
Breckenridge,
Final steps in exocytosis observed in a cell with giant secretory granules.
1987, Pubmed
Breckenridge,
Final steps in exocytosis observed in a cell with giant secretory granules.
1987,
Pubmed
Brooks,
Catecholamine secretion by chemically skinned cultured chromaffin cells.
1983,
Pubmed
Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase
Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Dunn,
Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells.
1983,
Pubmed
Elinson,
Fertilization in amphibians: the ancestry of the block to polyspermy.
1986,
Pubmed
Grey,
Formation and structure of the fertilization envelope in Xenopus laevis.
1974,
Pubmed
,
Xenbase
Hollinger,
Calcium-induced dehiscence of cortical granules in Xenopus laevis oocytes.
1979,
Pubmed
,
Xenbase
Holz,
Effects of osmolality and ionic strength on secretion from adrenal chromaffin cells permeabilized with digitonin.
1986,
Pubmed
Holz,
The role of osmotic forces in exocytosis from adrenal chromaffin cells.
1986,
Pubmed
Kilpatrick,
Stability of bovine adrenal medulla cells in culture.
1980,
Pubmed
Kline,
Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs.
1988,
Pubmed
,
Xenbase
Knight,
Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields.
1982,
Pubmed
Kopell,
Osmotic pressures of solutions of ATP and catecholamines relating to storage in chromaffin granules.
1982,
Pubmed
Kubota,
Free calcium wave upon activation in Xenopus eggs.
1987,
Pubmed
,
Xenbase
Lew,
Quantitative analysis of the cytosolic free calcium dependency of exocytosis from three subcellular compartments in intact human neutrophils.
1986,
Pubmed
Neher,
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
1982,
Pubmed
Njus,
Bioenergetics of secretory vesicles.
1986,
Pubmed
Patzak,
Exocytotic exposure and retrieval of membrane antigens of chromaffin granules: quantitative evaluation of immunofluorescence on the surface of chromaffin cells.
1984,
Pubmed
Phillips,
Visualization of the exocytosis/endocytosis secretory cycle in cultured adrenal chromaffin cells.
1983,
Pubmed
Wilson,
A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
1989,
Pubmed
Wilson,
Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells.
1983,
Pubmed
Winkler,
The molecular organization of adrenal chromaffin granules.
1980,
Pubmed
Winkler,
The composition of adrenal chromaffin granules: an assessment of controversial results.
1976,
Pubmed
Zimmerberg,
Simultaneous electrical and optical measurements show that membrane fusion precedes secretory granule swelling during exocytosis of beige mouse mast cells.
1987,
Pubmed
