XB-ART-24263FEBS Lett December 16, 1991; 295 (1-3): 185-8.
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Protein phosphatase 2A from Xenopus oocytes. Characterization during meiotic cell division.
A polyclonal antibody was raised against bacterially produced catalytic alpha subunit of protein phosphatase 2A (PP2AC) cloned from Xenopus ovarian library. The amount of PP2AC in Xenopus oocytes determined by Western blot analysis was 1 ng/microgram of cytosolic protein. The antibody depleted PP2AC from oocyte extracts in association with 6 components (40, 62, 65, 80, 85 and 90 kDa). Prophase- and metaphase-arrested oocytes contained identical amounts of PP2AC. Metaphase oocytes showed one specific change in the 62 kDa protein associated with PP2AC.
PubMed ID: 1662645
Article link: FEBS Lett
Species referenced: Xenopus
Genes referenced: ppp2ca ppp2cb ptpa