Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The polarized distribution of poly(A+)-mRNA-induced functional ion channels in the Xenopus oocyteplasma membrane is prevented by anticytoskeletal drugs.
Peter AB
,
Schittny JC
,
Niggli V
,
Reuter H
,
Sigel E
.
???displayArticle.abstract???
Foreign mRNA was expressed in Xenopus laevis oocytes. Newly expressed ion currents localized in defined plasma membrane areas were measured using the two-electrode voltage clamp technique in combination with a specially designed chamber, that exposed only part of the surface on the oocytes to channel agonists or inhibitors. Newly expressed currents were found to be unequally distributed in the surface membrane of the oocyte. This asymmetry was most pronounced during the early phase of expression, when channels could almost exclusively be detected in the animal hemisphere of the oocyte. 4 d after injection of the mRNA, or later, channels could be found at a threefold higher density at the animal than at the vegetal pole area. The pattern of distribution was observed to be similar with various ion channels expressed from crude tissue mRNA and from cRNAs coding for rat GABAA receptor channel subunits. Electron microscopical analysis revealed very similar microvilli patterns at both oocyte pole areas. Thus, the asymmetric current distribution is not due to asymmetric surface structure. Upon incubation during the expression period in either colchicine or cytochalasin D, the current density was found to be equal in both pole areas. The inactive control substance beta-lumicolchicine had no effect on the asymmetry of distribution. Colchicine was without effect on the amplitude of the expressed whole cell current. Our measurements reveal a pathway for plasma membrane protein expression endogenous to the Xenopus oocyte, that may contribute to the formation and maintenance of polarity of this highly organized cell.
Achler,
Role of microtubules in polarized delivery of apical membrane proteins to the brush border of the intestinal epithelium.
1989, Pubmed
Achler,
Role of microtubules in polarized delivery of apical membrane proteins to the brush border of the intestinal epithelium.
1989,
Pubmed
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Bartles,
Plasma membrane protein sorting in epithelial cells: do secretory pathways hold the key?
1988,
Pubmed
Cathala,
A method for isolation of intact, translationally active ribonucleic acid.
1983,
Pubmed
Ceriotti,
Binding to membrane proteins within the endoplasmic reticulum cannot explain the retention of the glucose-regulated protein GRP78 in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Colman,
Meiotic maturation in Xenopus oocytes: a link between the cessation of protein secretion and the polarized disappearance of Golgi apparati.
1985,
Pubmed
,
Xenbase
Colman,
Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis.
1981,
Pubmed
,
Xenbase
Cooper,
Effects of cytochalasin and phalloidin on actin.
1987,
Pubmed
Dawid,
Xenopus laevis in developmental and molecular biology.
1988,
Pubmed
,
Xenbase
Dotti,
Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture.
1990,
Pubmed
Dreyfus,
Tissue-specific processing and polarized compartmentalization of clone-produced cholinesterase in microinjected Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Drummond,
Stability and movement of mRNAs and their encoded proteins in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Durand-Schneider,
Effect of colchicine and phalloidin on the distribution of three plasma membrane antigens in rat hepatocytes: comparison with bile duct ligation.
1987,
Pubmed
Lane,
The fate of genes, messengers, and proteins introduced into Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Malherbe,
GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands.
1990,
Pubmed
,
Xenbase
Matter,
Microtubule perturbation retards both the direct and the indirect apical pathway but does not affect sorting of plasma membrane proteins in intestinal epithelial cells (Caco-2).
1990,
Pubmed
Matus-Leibovitch,
Two types of intrinsic muscarinic responses in Xenopus oocytes. II. Hemispheric asymmetry of responses and receptor distribution.
1990,
Pubmed
,
Xenbase
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Oron,
Differences in receptor-evoked membrane electrical responses in native and mRNA-injected Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Poo,
Rapid lateral diffusion of functional A Ch receptors in embryonic muscle cell membrane.
1982,
Pubmed
,
Xenbase
Rindler,
Microtubule-acting drugs lead to the nonpolarized delivery of the influenza hemagglutinin to the cell surface of polarized Madin-Darby canine kidney cells.
1987,
Pubmed
Robinson,
Electrical currents through full-grown and maturing Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Salas,
Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity.
1986,
Pubmed
Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase
Sigel,
Activation of protein kinase C differentially modulates neuronal Na+, Ca2+, and gamma-aminobutyrate type A channels.
1988,
Pubmed
,
Xenbase
Sigel,
Effects of veratridine on single neuronal sodium channels expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Simons,
Polarized sorting in epithelia.
1990,
Pubmed
Soreq,
The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Strous,
Vesicular stomatitis virus glycoprotein, albumin, and transferrin are transported to the cell surface via the same Golgi vesicles.
1983,
Pubmed