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The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities.
Burd CG
,
Matunis EL
,
Dreyfuss G
.
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The poly(A)-binding protein (PABP) is the major mRNA-binding protein in eukaryotes, and it is essential for viability of the yeast Saccharomyces cerevisiae. The amino acid sequence of the protein indicates that it consists of four ribonucleoprotein consensus sequence-containing RNA-binding domains (RBDs I, II, III, and IV) and a proline-rich auxiliary domain at the carboxyl terminus. We produced different parts of the S. cerevisiae PABP and studied their binding to poly(A) and other ribohomopolymers in vitro. We found that none of the individual RBDs of the protein bind poly(A) specifically or efficiently. Contiguous two-domain combinations were required for efficient RNA binding, and each pairwise combination (I/II, II/III, and III/IV) had a distinct RNA-binding activity. Specific poly(A)-binding activity was found only in the two amino-terminal RBDs (I/II) which, interestingly, are dispensable for viability of yeast cells, whereas the activity that is sufficient to rescue lethality of a PABP-deleted strain is in the carboxyl-terminal RBDs (III/IV). We conclude that the PABP is a multifunctional RNA-binding protein that has at least two distinct and separable activities: RBDs I/II, which most likely function in binding the PABP to mRNA through the poly(A) tail, and RBDs III/IV, which may function through binding either to a different part of the same mRNA molecule or to other RNA(s).
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986, Pubmed
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986,
Pubmed
Bandziulis,
RNA-binding proteins as developmental regulators.
1989,
Pubmed
Bernstein,
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.
1989,
Pubmed
Blobel,
A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.
1973,
Pubmed
Brewer,
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.
1988,
Pubmed
Burd,
Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts.
1989,
Pubmed
Dreyfuss,
Heterogeneous nuclear ribonucleoprotein particles and the pathway of mRNA formation.
1988,
Pubmed
Dreyfuss,
Structure and function of nuclear and cytoplasmic ribonucleoprotein particles.
1986,
Pubmed
Grange,
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
1987,
Pubmed
,
Xenbase
Konarska,
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.
1986,
Pubmed
Lutz-Freyermuth,
Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA.
1990,
Pubmed
Munroe,
mRNA poly(A) tail, a 3' enhancer of translational initiation.
1990,
Pubmed
Nietfeld,
The Xenopus laevis poly(A) binding protein is composed of multiple functionally independent RNA binding domains.
1990,
Pubmed
,
Xenbase
Piñol-Roma,
Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.
1988,
Pubmed
Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed
Sachs,
The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
1989,
Pubmed
Sachs,
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
1987,
Pubmed
Sachs,
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
1986,
Pubmed
Sachs,
Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46.
1990,
Pubmed
Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase
Scherly,
Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.
1990,
Pubmed
,
Xenbase
Swanson,
Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
1987,
Pubmed
Swanson,
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
1988,
Pubmed
Zelus,
Expression of the poly(A)-binding protein during development of Xenopus laevis.
1989,
Pubmed
,
Xenbase
