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Inhibition of translation initiation by antisense oligonucleotides via an RNase-H independent mechanism.
Boiziau C
,
Kurfurst R
,
Cazenave C
,
Roig V
,
Thuong NT
,
Toulmé JJ
.
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We have used alpha-oligomers as antisense oligonucleotides complementary to three different sequences of the rabbit beta-globin mRNA: a region adjacent to the cap site, a region spanning the AUG initiation codon or a sequence in the coding region. These alpha-oligonucleotides were synthesized either with a free 5' OH group or linked to an acridine derivative. The effect of these oligonucleotides on mRNA translation was investigated in cell-free extracts and in Xenopus oocytes. In rabbit reticulocyte lysate and in wheat germ extracts oligomers targeted to the cap site and the initiation codon reduced beta-globin synthesis in a dose-dependent manner, whereas the target mRNA remained intact. The anti-cap alpha-oligomer was even more efficient that its beta-counterpart in rabbit reticulocyte lysate. In contrast, only the alpha-oligomer, linked to the acridine derivative, complementary to the cap region displayed significant antisense properties in Xenopus oocytes. Therefore initiation of translation can be arrested by oligonucleotide/RNA hybrids which are not substrates for RNase-H.
Asseline,
Nucleic acid-binding molecules with high affinity and base sequence specificity: intercalating agents covalently linked to oligodeoxynucleotides.
1984, Pubmed
Asseline,
Nucleic acid-binding molecules with high affinity and base sequence specificity: intercalating agents covalently linked to oligodeoxynucleotides.
1984,
Pubmed
Bertrand,
Comparative activity of alpha- and beta-anomeric oligonucleotides on rabbit beta globin synthesis: inhibitory effect of cap targeted alpha-oligonucleotides.
1989,
Pubmed
Blake,
Hybridization arrest of globin synthesis in rabbit reticulocyte lysates and cells by oligodeoxyribonucleoside methylphosphonates.
1985,
Pubmed
Büsen,
Distinct ribonuclease H activities in calf thymus.
1975,
Pubmed
Cazenave,
Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit beta-globin synthesis in wheat germ extracts and Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Cazenave,
Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.
1987,
Pubmed
,
Xenbase
Cazenave,
Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies.
1987,
Pubmed
,
Xenbase
Cazenave,
Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides.
1989,
Pubmed
,
Xenbase
Chang,
Comparative inhibition of ras p21 protein synthesis with phosphorus-modified antisense oligonucleotides.
1989,
Pubmed
Dash,
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
1987,
Pubmed
,
Xenbase
Gagnor,
Alpha-DNA.IX: Parallel annealing of alpha-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription.
1989,
Pubmed
Gagnor,
alpha-DNA. VI: Comparative study of alpha- and beta-anomeric oligodeoxyribonucleotides in hybridization to mRNA and in cell free translation inhibition.
1987,
Pubmed
Goodchild,
Inhibition of rabbit beta-globin synthesis by complementary oligonucleotides: identification of mRNA sites sensitive to inhibition.
1988,
Pubmed
Haeuptle,
Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length.
1986,
Pubmed
Hélène,
Specific regulation of gene expression by antisense, sense and antigene nucleic acids.
1990,
Pubmed
Kean,
Photochemical cross-linking of psoralen-derivatized oligonucleoside methylphosphonates to rabbit globin messenger RNA.
1988,
Pubmed
Lawson,
Influence of 5' proximal secondary structure on the translational efficiency of eukaryotic mRNAs and on their interaction with initiation factors.
1986,
Pubmed
Maher,
Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylphosphonates in a cell-free system.
1988,
Pubmed
Marcus-Sekura,
Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages.
1987,
Pubmed
Minshull,
The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.
1986,
Pubmed
Morvan,
alpha-DNA II. Synthesis of unnatural alpha-anomeric oligodeoxyribonucleotides containing the four usual bases and study of their substrate activities for nucleases.
1987,
Pubmed
Rovera,
Resolution of hemoglobin subunits by electrophoresis in acid urea polyacrylamide gels containing Triton X-100.
1978,
Pubmed
Shakin,
Destabilization of messenger RNA/complementary DNA duplexes by the elongating 80 S ribosome.
1986,
Pubmed
Shuttleworth,
Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.
1988,
Pubmed
,
Xenbase
Smith,
Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.
1986,
Pubmed
Smith,
Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation.
1988,
Pubmed
,
Xenbase
Sun,
Oligo-[alpha]-deoxynucleotides covalently linked to an intercalating agent. Double helices with parallel strands are formed with complementary oligo-[beta]-deoxynucleotides.
1987,
Pubmed
Thuong,
Oligo(alpha-deoxynucleotide)s covalently linked to intercalating agents: differential binding to ribo- and deoxyribopolynucleotides and stability towards nuclease digestion.
1987,
Pubmed
Tidd,
Evaluation of N-ras oncogene anti-sense, sense and nonsense sequence methylphosphonate oligonucleotide analogues.
1988,
Pubmed
Toulmé,
Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.
1986,
Pubmed
Vasanthakumar,
Modulation of drug resistance in a daunorubicin resistant subline with oligonucleoside methylphosphonates.
1989,
Pubmed
Verspieren,
An acridine-linked oligodeoxynucleotide targeted to the common 5' end of trypanosome mRNAs kills cultured parasites.
1987,
Pubmed
Verspieren,
Effect of RNA secondary structure and modified bases on the inhibition of trypanosomatid protein synthesis in cell free extracts by antisense oligodeoxynucleotides.
1990,
Pubmed
Walder,
Role of RNase H in hybrid-arrested translation by antisense oligonucleotides.
1988,
Pubmed
van der Krol,
Modulation of eukaryotic gene expression by complementary RNA or DNA sequences.
1988,
Pubmed