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J Neurophysiol
2005 May 01;935:2541-51. doi: 10.1152/jn.01283.2004.
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Pharmacology of acetylcholine-mediated cell signaling in the lateral line organ following efferent stimulation.
Dawkins R
,
Keller SL
,
Sewell WF
.
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Cholinergic efferent fibers modify hair cell responses to mechanical stimulation. It is hypothesized that calcium entering the hair cell through a nicotinic receptor activates a small-conductance (SK), calcium-activated potassium channel to hyperpolarize the hair cell. The calcium signal may be amplified by calcium-induced calcium release from the synaptic cisternae. Pharmacological tests of these ideas in the intact cochlea have been technically difficult because of the complex and fragile structure of the mammalian inner ear. We turned to the Xenopus laevis lateral line organ, whose simplicity and accessibility make it a model for understanding hair cell organ function in a relatively intact system. Drugs were applied to the inner surface of the skin while monitoring the effects of efferent stimulation on afferent fiber discharge rate. Efferent effects were blocked by antagonists of SK channels including apamin (EC50 = 0.5 microM) and dequalinium (EC50 = 12 microM). The effect of apamin was not enhanced by co-administration of phenylmethylsulfonyl fluoride, a proteolysis inhibitor. Efferent effects were attenuated by ryanodine, an agent that can interfere with calcium-induced calcium release, although relatively high (mM) concentrations of ryanodine were required. Fluorescent cationic styryl dyes, 4-di-2-asp and fm 1-43, blocked efferent effects, although it was not possible to observe specific entry of the dye into the base of hair cells. These pharmacological findings in the Xenopus lateral line organ support the hypothesis that effects of efferent stimulation are mediated by calcium entry through the nicotinic receptor via activation of SK channels and suggest the generality of this mechanism in meditating cholinergic efferent effects.
Art,
Efferent regulation of hair cells in the turtle cochlea.
1982, Pubmed
Art,
Efferent regulation of hair cells in the turtle cochlea.
1982,
Pubmed
Art,
Synaptic hyperpolarization and inhibition of turtle cochlear hair cells.
1984,
Pubmed
Bailey,
Calcitonin gene-related peptide suppresses hair cell responses to mechanical stimulation in the Xenopus lateral line organ.
2000,
Pubmed
,
Xenbase
Balak,
Regenerated hair cells can originate from supporting cell progeny: evidence from phototoxicity and laser ablation experiments in the lateral line system.
1990,
Pubmed
Blanchet,
Gentamicin blocks ACh-evoked K+ current in guinea-pig outer hair cells by impairing Ca2+ entry at the cholinergic receptor.
2000,
Pubmed
Blanchet,
Acetylcholine-induced potassium current of guinea pig outer hair cells: its dependence on a calcium influx through nicotinic-like receptors.
1996,
Pubmed
Bobbin,
Caffeine and ryanodine demonstrate a role for the ryanodine receptor in the organ of Corti.
2002,
Pubmed
Bobbin,
Comparative actions of GABA and acetylcholine on the Xenopus laevis lateral line.
1985,
Pubmed
,
Xenbase
Coronado,
Structure and function of ryanodine receptors.
1994,
Pubmed
Dawkins,
Afferent synaptic transmission in a hair cell organ: pharmacological and physiological analysis of the role of the extended refractory period.
2004,
Pubmed
,
Xenbase
Drescher,
Spontaneous neural activity of a mechanoreceptive system is undiminished by replacement of external calcium with equimolar magnesium in the presence of EGTA.
1987,
Pubmed
,
Xenbase
Drescher,
Calcium and magnesium dependence of spontaneous and evoked afferent neural activity in the lateral-line organ of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Dulon,
Expression of small-conductance calcium-activated potassium channels (SK) in outer hair cells of the rat cochlea.
1998,
Pubmed
Dunn,
Ganglion-blocking activity of dequalinium in frog and rat sympathetic ganglia in vitro.
1993,
Pubmed
Elgoyhen,
alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells.
2001,
Pubmed
,
Xenbase
Elgoyhen,
Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells.
1994,
Pubmed
,
Xenbase
Evans,
Cholinergic control of membrane conductance and intracellular free Ca2+ in outer hair cells of the guinea pig cochlea.
2000,
Pubmed
Flock,
Inhibition by efferent nerve fibres: action on hair cells and afferent synaptic transmission in the lateral line canal organ of the burbot Lota lota.
1976,
Pubmed
Flock,
Vital staining of the hearing organ: visualization of cellular structure with confocal microscopy.
1998,
Pubmed
Fuchs,
Cholinergic inhibition of short (outer) hair cells of the chick's cochlea.
1992,
Pubmed
Gale,
FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel.
2001,
Pubmed
Glowatzki,
Cholinergic synaptic inhibition of inner hair cells in the neonatal mammalian cochlea.
2000,
Pubmed
Guth,
Effects of divalent cations on the frequency of spontaneous action potentials from the lateral line organ of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Harris,
Input-output characteristics of the lateral-line sense organs of Xenopus laevis.
1966,
Pubmed
,
Xenbase
Hendricson,
Transmitter release from Rana pipiens vestibular hair cells via mGluRs: a role for intracellular Ca(++) release.
2002,
Pubmed
Holt,
The effect of proteolytic enzymes on the alpha9-nicotinic receptor-mediated response in isolated frog vestibular hair cells.
2001,
Pubmed
Housley,
Cholinergically-induced changes in outward currents in hair cells isolated from the semicircular canal of the frog.
1990,
Pubmed
Housley,
Direct measurement of the action of acetylcholine on isolated outer hair cells of the guinea pig cochlea.
1991,
Pubmed
Katz,
Developmental regulation of nicotinic synapses on cochlear inner hair cells.
2004,
Pubmed
Kennedy,
Fast Ca2+ signals at mouse inner hair cell synapse: a role for Ca2+-induced Ca2+ release.
2002,
Pubmed
Kroese,
Frequency response of the lateral-line organ of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Le Calvez,
An in vitro preparation to access cellular and neuronal components in the mouse inner ear.
2000,
Pubmed
Lelli,
Presynaptic calcium stores modulate afferent release in vestibular hair cells.
2003,
Pubmed
Lioudyno,
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells.
2004,
Pubmed
Martin,
The dependence of calcium-activated potassium currents on membrane potential.
1992,
Pubmed
Meyers,
Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels.
2003,
Pubmed
Morley,
Developmental mRNA expression of the alpha10 nicotinic acetylcholine receptor subunit in the rat cochlea.
2002,
Pubmed
Nagasaki,
Ryanodine sensitivity of the calcium release channel of sarcoplasmic reticulum.
1988,
Pubmed
Nenov,
Acetylcholine response in guinea pig outer hair cells. II. Activation of a small conductance Ca(2+)-activated K+ channel.
1996,
Pubmed
Nie,
Cloning and expression of a small-conductance Ca(2+)-activated K+ channel from the mouse cochlea: coexpression with alpha9/alpha10 acetylcholine receptors.
2004,
Pubmed
,
Xenbase
Nishikawa,
Internalization of styryl dye FM1-43 in the hair cells of lateral line organs in Xenopus larvae.
1996,
Pubmed
,
Xenbase
Oliver,
Gating of Ca2+-activated K+ channels controls fast inhibitory synaptic transmission at auditory outer hair cells.
2000,
Pubmed
Park,
Ion selectivity and gating of small conductance Ca(2+)-activated K+ channels in cultured rat adrenal chromaffin cells.
1994,
Pubmed
Protasi,
All three ryanodine receptor isoforms generate rapid cooling responses in muscle cells.
2004,
Pubmed
Rothlin,
Direct interaction of serotonin type 3 receptor ligands with recombinant and native alpha 9 alpha 10-containing nicotinic cholinergic receptors.
2003,
Pubmed
,
Xenbase
Rousseau,
Ryanodine modifies conductance and gating behavior of single Ca2+ release channel.
1987,
Pubmed
Russell,
The role of the lateral-line efferent system in Xenopus laevis.
1971,
Pubmed
,
Xenbase
Russell,
The pharmacology of efferent synapses in the lateral-line system of Xenopus laevis.
1971,
Pubmed
,
Xenbase
Seiler,
Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants.
1999,
Pubmed
Sewell,
Synaptic potentials in afferent fibers innervating hair cells of the lateral line organ in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Sewell,
Effects of calcitonin gene-related peptide and efferent nerve stimulation on afferent transmission in the lateral line organ.
1991,
Pubmed
,
Xenbase
Shelton,
The structure and function of the lateral line system in larval Xenopus laevis.
1971,
Pubmed
,
Xenbase
Si,
Developmental assembly of transduction apparatus in chick basilar papilla.
2003,
Pubmed
Skau,
Phenylmethylsulfonyl fluoride inhibitory effects on acetylcholinesterase of brain and muscle.
1999,
Pubmed
Sridhar,
Unique postsynaptic signaling at the hair cell efferent synapse permits calcium to evoke changes on two time scales.
1997,
Pubmed
Strelioff,
Neural transduction in Xenopus laevis lateral line system.
1978,
Pubmed
,
Xenbase
Strøbaek,
Pharmacological characterization of small-conductance Ca(2+)-activated K(+) channels stably expressed in HEK 293 cells.
2000,
Pubmed
Turini,
The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin.
1969,
Pubmed
Weisstaub,
The alpha9alpha10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations.
2002,
Pubmed
,
Xenbase
Yamamoto,
Effects of potassium channel blockers on the acetylcholine-induced currents in dissociated outer hair cells of guinea pig cochlea.
1997,
Pubmed
Yoshida,
Gentamicin blocks both fast and slow effects of olivocochlear activation in anesthetized guinea pigs.
1999,
Pubmed
Yoshida,
Fast, but not slow, effects of olivocochlear activation are resistant to apamin.
2001,
Pubmed
Yoshida,
The role of inositol trisphosphate on ACh-induced outward currents in bullfrog saccular hair cells.
1994,
Pubmed
Yuhas,
Apamin-sensitive, small-conductance, calcium-activated potassium channels mediate cholinergic inhibition of chick auditory hair cells.
1999,
Pubmed