Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure of a ribosomal protein gene in Mucor racemosus.
Sosa L
,
Fonzi WA
,
Sypherd PS
.
???displayArticle.abstract???
As an extension of our analysis of the translational apparatus of Mucor racemosus we have isolated a gene encoding a ribosomal protein of Mucor. Based on a method developed for S. cerevisiae, we identified by hybrid selection and in vitro translation a lambda-Charon 4A clone containing the genomic copy of a Mucor ribosomal protein. The gene consisted of two exons of 57 and 387 nucleotides. The two exons were separated by an 131 nucleotide intron. The processed transcript was 714 nucleotides in length and contained a 25 nucleotide untranscribed leader and an 114 nucleotide untranscribed 3'-end. The protein predicted from the nucleotide sequence contained 148 amino acids and exhibited 61% identity with the S19 ribosomal protein of Xenopus laevis. The promoter region of the gene contained sequences highly homologous to the RPG and Homol1 promoter elements found in S. cerevisiae. Southern blot analysis indicated that the Mucor genome contains three copies of this gene.
Amaldi,
Nucleotide sequences of cloned cDNA fragments specific for six Xenopus laevis ribosomal proteins.
1982, Pubmed,
Xenbase
Amaldi,
Nucleotide sequences of cloned cDNA fragments specific for six Xenopus laevis ribosomal proteins.
1982,
Pubmed
,
Xenbase
Bollen,
Ribosomal protein genes of yeast contain intervening sequences.
1982,
Pubmed
Calzone,
Mapping of gene transcripts by nuclease protection assays and cDNA primer extension.
1987,
Pubmed
Chen,
Homologous ribosomal proteins in bacteria, yeast, and humans.
1986,
Pubmed
Cihlar,
The organization of the ribosomal RNA genes in the fungus Mucor racemosus.
1980,
Pubmed
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Fonzi,
Regulation of protein synthesis factor EF-1 alpha in Mucor racemosus.
1985,
Pubmed
Hamilton,
Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs.
1987,
Pubmed
Hu,
Mapping transcription start points on cloned genomic DNA with T4 DNA polymerase: a precise and convenient technique.
1986,
Pubmed
Huet,
A general upstream binding factor for genes of the yeast translational apparatus.
1985,
Pubmed
Huet,
TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain.
1987,
Pubmed
Kimura,
Primary structures of three highly acidic ribosomal proteins S6, S12 and S15 from the archaebacterium Halobacterium marismortui.
1987,
Pubmed
,
Xenbase
Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed
Larsen,
Ribosomal proteins of the dimorphic fungus, Mucor racemosus.
1979,
Pubmed
Larsen,
Physiological control of phosphorylation ribosomal protein S6 in Mucor racemosus.
1980,
Pubmed
Leer,
Conserved sequences upstream of yeast ribosomal protein genes.
1985,
Pubmed
Linz,
Three genes for the elongation factor EF-1 alpha in Mucor racemosus.
1986,
Pubmed
Nagata,
Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.
1984,
Pubmed
Nieuwint,
The gene for yeast ribosomal protein S31 contains an intron in the leader sequence.
1985,
Pubmed
Nischt,
Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe.
1987,
Pubmed
Orlowski,
Regulation of translation rate during morphogenesis in the fungus Mucor.
1978,
Pubmed
Parnes,
Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.
1981,
Pubmed
Pearson,
Yeast use translational control to compensate for extra copies of a ribosomal protein gene.
1982,
Pubmed
Rosbash,
The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
1981,
Pubmed
Rotenberg,
Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes.
1986,
Pubmed
Sanger,
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
1975,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Tanaka,
Nucleotide sequence of cloned cDNA specific for rat ribosomal protein S11.
1985,
Pubmed
Teem,
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
1983,
Pubmed
Teem,
A comparison of yeast ribosomal protein gene DNA sequences.
1984,
Pubmed
Thomas,
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
1983,
Pubmed
,
Xenbase
Wagner,
Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.
1985,
Pubmed
Wessel,
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
1984,
Pubmed
Woolford,
Isolation of cloned DNA sequences containing ribosomal protein genes from Saccharomyces cerevisiae.
1979,
Pubmed
Woudt,
Conserved sequence elements upstream of the gene encoding yeast ribosomal protein L25 are involved in transcription activation.
1986,
Pubmed
Woudt,
Analysis of upstream activation sites of yeast ribosomal protein genes.
1987,
Pubmed
Woudt,
Structural and putative regulatory sequences of the gene encoding ribosomal protein L25 in Candida utilis.
1987,
Pubmed
