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Evolutionary conservation of the biochemical properties of p53: specific interaction of Xenopus laevis p53 with simian virus 40 large T antigen and mammalian heat shock proteins 70.
Soussi T
,
Caron de Fromentel C
,
Stürzbecher HW
,
Ullrich S
,
Jenkins J
,
May P
.
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We have investigated the biochemical properties of Xenopus laevis p53. With an in vitro binding assay, we can detect a specific association between X. laevis p53 and simian virus 40 large T antigen. Furthermore, X. laevis p53 expressed in monkey COS cells is stably associated with this viral antigen. Like mammalian p53, X. laevis p53 in complex with simian virus 40 large T antigen exhibits a 20-fold increase of its half-life. On the other hand, X. laevis p53 is unable to associate either in vivo or in vitro with adenovirus type 5 E1B 55-kilodalton protein. We show by an immunological technique that X. laevis p53 forms specific complexes with mammalian hsp72 and hsp73 heat shock proteins only at a temperature well above the optimal growth temperature for X. laevis. Our results suggest that the protein-binding properties of p53 are closely related to the functional activity of the protein.
Benoist,
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
1980, Pubmed
Benoist,
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
1980,
Pubmed
Caron de Fromentel,
SV40 large T-antigen and transformation related protein p53 are associated in situ with nuclear RNP structures containing hnRNA of transformed cells.
1986,
Pubmed
Caron de Fromentel,
Presence of circulating antibodies against cellular protein p53 in a notable proportion of children with B-cell lymphoma.
1987,
Pubmed
Carroll,
Time-dependent maturation of the simian virus 40 large T antigen-p53 complex studied by using monoclonal antibodies.
1982,
Pubmed
Clarke,
Purification of complexes of nuclear oncogene p53 with rat and Escherichia coli heat shock proteins: in vitro dissociation of hsc70 and dnaK from murine p53 by ATP.
1988,
Pubmed
Crawford,
Detection of antibodies against the cellular protein p53 in sera from patients with breast cancer.
1982,
Pubmed
DeLeo,
Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
1979,
Pubmed
Deppert,
Evidence for free and metabolically stable p53 protein in nuclear subfractions of simian virus 40-transformed cells.
1986,
Pubmed
Deppert,
Modulation of p53 protein expression during cellular transformation with simian virus 40.
1987,
Pubmed
Ehrhart,
Specific interaction between a subset of the p53 protein family and heat shock proteins hsp72/hsc73 in a human osteosarcoma cell line.
1988,
Pubmed
Eliyahu,
Participation of p53 cellular tumour antigen in transformation of normal embryonic cells.
,
Pubmed
Ernoult-Lange,
Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence.
1984,
Pubmed
Finlay,
Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life.
1988,
Pubmed
Gannon,
p53 and DNA polymerase alpha compete for binding to SV40 T antigen.
,
Pubmed
Gaudray,
Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotype.
1978,
Pubmed
Gluzman,
SV40-transformed simian cells support the replication of early SV40 mutants.
1981,
Pubmed
Gronostajski,
Energy requirement for degradation of tumor-associated protein p53.
1984,
Pubmed
Gurney,
Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens.
1980,
Pubmed
Harlow,
Monoclonal antibodies specific for simian virus 40 tumor antigens.
1981,
Pubmed
Harlow,
Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.
1985,
Pubmed
Harrison,
Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
1977,
Pubmed
Hinds,
Immunological evidence for the association of p53 with a heat shock protein, hsc70, in p53-plus-ras-transformed cell lines.
1987,
Pubmed
Jenkins,
Cloning and expression analysis of full length mouse cDNA sequences encoding the transformation associated protein p53.
1984,
Pubmed
Jenkins,
Cellular immortalization by a cDNA clone encoding the transformation-associated phosphoprotein p53.
,
Pubmed
Jenkins,
The cellular oncogene p53 can be activated by mutagenesis.
,
Pubmed
Jenkins,
Two distinct regions of the murine p53 primary amino acid sequence are implicated in stable complex formation with simian virus 40 T antigen.
1988,
Pubmed
,
Xenbase
Kress,
Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
1979,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lane,
T antigen is bound to a host protein in SV40-transformed cells.
1979,
Pubmed
Linzer,
Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.
1979,
Pubmed
Manteuil,
Replication of simian virus 40 deoxyribonucleic acid: analysis of the one-step growth cycle.
1973,
Pubmed
Matlashewski,
Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.
1984,
Pubmed
McCormick,
SV40 T antigen binds specifically to a cellular 53 K protein in vitro.
1981,
Pubmed
Melero,
Identification of new polypeptide species (48-55K) immunoprecipitable by antiserum to purified large T antigen and present in SV40-infected and -transformed cells.
1979,
Pubmed
Oren,
Molecular cloning of a cDNA specific for the murine p53 cellular tumor antigen.
1983,
Pubmed
Oren,
Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells.
1981,
Pubmed
Parada,
Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation.
,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Pennica,
The amino acid sequence of murine p53 determined from a c-DNA clone.
1984,
Pubmed
Pinhasi,
Expression of the mouse p53 cellular tumor antigen in monkey cells.
1984,
Pubmed
Pinhasi-Kimhi,
Specific interaction between the p53 cellular tumour antigen and major heat shock proteins.
,
Pubmed
Reich,
Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53.
1983,
Pubmed
Rotter,
Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50.
1980,
Pubmed
Ruscetti,
Expression of a transformation-related protein (p53) in the malignant stage of Friend virus-induced diseases.
1983,
Pubmed
Sarnow,
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
1982,
Pubmed
Soussi,
Cloning and characterization of a cDNA from Xenopus laevis coding for a protein homologous to human and murine p53.
1987,
Pubmed
,
Xenbase
Soussi,
Nucleotide sequence of a cDNA encoding the chicken p53 nuclear oncoprotein.
1988,
Pubmed
Soussi,
Nucleotide sequence of a cDNA encoding the rat p53 nuclear oncoprotein.
1988,
Pubmed
Stürzbecher,
Characterization of mutant p53-hsp72/73 protein-protein complexes by transient expression in monkey COS cells.
1988,
Pubmed
Stürzbecher,
Mutant p53 proteins bind hsp 72/73 cellular heat shock-related proteins in SV40-transformed monkey cells.
1987,
Pubmed
Tan,
Identification of the p53 protein domain involved in formation of the simian virus 40 large T-antigen-p53 protein complex.
1986,
Pubmed
Wolf,
In vitro expression of human p53 cDNA clones and characterization of the cloned human p53 gene.
1985,
Pubmed
Zakut-Houri,
A single gene and a pseudogene for the cellular tumour antigen p53.
,
Pubmed
Zakut-Houri,
Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells.
1985,
Pubmed
Zantema,
Adenovirus serotype determines association and localization of the large E1B tumor antigen with cellular tumor antigen p53 in transformed cells.
1985,
Pubmed
