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Reconstitution of the Golgi apparatus after microinjection of rat liver Golgi fragments into Xenopus oocytes.
Paiement J
,
Jolicoeur M
,
Fazel A
,
Bergeron JJ
.
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We have studied the reconstitution of the Golgi apparatus in vivo using an heterologous membrane transplant system. Endogenous glycopeptides of rat hepatic Golgi fragments were radiolabeled in vitro with [3H]sialic acid using detergent-free conditions. The Golgi fragments consisting of dispersed vesicles and tubules with intraluminal lipoprotein-like particles were then microinjected into Xenopus oocytes and their fate studied by light (LM) and electron microscope (EM) radioautography. 3 h after microinjection, radiolabel was observed by LM radioautography over yolk platelet-free cytoplasmic regions near the injection site. EM radioautography revealed label over Golgi stacked saccules containing the hepatic marker of intraluminal lipoprotein-like particles. At 14 h after injection, LM radioautographs revealed label in the superficial cortex of the oocytes between the yolk platelets and at the oocyte surface. EM radioautography identified the labeled structures as the stacked saccules of the Golgi apparatus, the oocyte cortical granules, and the plasmalemma, indicating that a proportion of microinjected material was transferred to the surface via the secretion pathway of the oocyte. The efficiency of transport was low, however, as biochemical studies failed to show extensive secretion of radiolabel into the extracellular medium by 14 h with approximately half the microinjected radiolabeled constituents degraded. Vinblastine (50 microM) administered to oocytes led to the formation of tubulin paracrystals. Although microinjected Golgi fragments were able to effect the formation of stacked saccules in vinblastine-treated oocytes, negligible transfer of heterologous material to the oocyte surface could be detected by radioautography. The data demonstrate that dispersed fragments of the rat liver Golgi complex (i.e., unstacked vesicles and tubules) reconstitute into stacked saccules when microinjected into Xenopus cytoplasm. After the formation of stacked saccules, reconstituted Golgi fragments transport constituents into a portion of the exocytic pathway of the host cell by a microtubule-regulated process.
Balch,
Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
1984, Pubmed
Balch,
Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
1984,
Pubmed
Bennett,
Influence of colchicine and vinblastine on the intracellular migration of secretory and membrane glycoproteins: III. Inhibition of intracellular migration of membrane glycoproteins in rat intestinal columnar cells and hepatocytes as visualized by light and electron-microscope radioautography after 3H-fucose injection.
1984,
Pubmed
Bergeron,
Terminal glycosylation in rat hepatic Golgi fractions: heterogeneous locations for sialic acid and galactose acceptors and their transferases.
1985,
Pubmed
Bergeron,
Golgi fractions from livers of control and ethanol-intoxicated rats. Enzymic and morphologic properties following rapid isolation.
1979,
Pubmed
Bergeron,
Differential and analytical subfractionation of rat liver components internalizing insulin and prolactin.
1986,
Pubmed
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Colman,
Meiotic maturation in Xenopus oocytes: a link between the cessation of protein secretion and the polarized disappearance of Golgi apparati.
1985,
Pubmed
,
Xenbase
Drummond,
Stability and movement of mRNAs and their encoded proteins in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Ehrenreich,
Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.
1973,
Pubmed
Featherstone,
Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells.
1985,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Howell,
Hepatic Golgi fractions resolved into membrane and content subfractions.
1982,
Pubmed
Kalt,
A study of fixation of early amphibian embryos for electron microscopy.
1971,
Pubmed
,
Xenbase
Kay,
In vivo uptake of insulin into hepatic Golgi fractions: application of the diaminobenzidine-shift protocol.
1984,
Pubmed
Khan,
Internalization of insulin into rat liver Golgi elements. Evidence for vesicle heterogeneity and the path of intracellular processing.
1982,
Pubmed
Kopriwa,
A reliable, standardized method for ultrastructural electron microscopic radioautography.
1973,
Pubmed
Kopriwa,
A comparison of various procedures for fine grain development in electron microscopic radioautography.
1975,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Le Marchand,
A role for the microtubular system in the release of very low density lipoproteins by perfused mouse livers.
1973,
Pubmed
Masui,
Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.
1967,
Pubmed
Melançon,
Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
1987,
Pubmed
Paiement,
Morphology of endoplasmic reticulum and Golgi elements following microinjection of rat liver microsomes into Xenopus laevis oocyte cytoplasm.
1986,
Pubmed
,
Xenbase
Paiement,
Properties of a GTP sensitive microdomain in rough microsomes.
1987,
Pubmed
Paiement,
Cytochemical analysis of the reconstitution of endoplasmic reticulum after microinjection of rat liver microsomes into Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Paiement,
Membrane fusion and glycosylation in the rat hepatic Golgi apparatus.
1982,
Pubmed
Paiement,
Physiological concentrations of GTP stimulate fusion of the endoplasmic reticulum and the nuclear envelope.
1984,
Pubmed
Reaven,
Evidence that microtubules play a permissive role in hepatocyte very low density lipoprotein secretion.
1980,
Pubmed
Redman,
Colchicine inhibition of plasma protein release from rat hepatocytes.
1975,
Pubmed
Rothman,
Intercompartmental transport in the Golgi complex is a dissociative process: facile transfer of membrane protein between two Golgi populations.
1984,
Pubmed
Thyberg,
Microtubules and the organization of the Golgi complex.
1985,
Pubmed
