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Cell Tissue Res
1988 Nov 01;2542:415-20. doi: 10.1007/bf00225814.
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Presence of microtubules in isolated cortices of prophase I and metaphase II oocytes in Xenopus laevis.
Huchon D
,
Jessus C
,
Thibier C
,
Ozon R
.
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An extensive array of microtubules has been shown to exist in the cortex of Xenopus laevis oocytes both at the prophase I and metaphase II stages. The cortical microtubules were visualized after the oocyte cortex was squashed and immunostained using anti-tubulin antibody. They are cold- and nocodazole-sensitive; their stability to both treatments decreases after meiotic maturation. Biochemical extraction of manually isolated oocyte cortices, in a microtubule-stabilizing buffer, confirms these cytological observations.
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