Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1988 Aug 01;78:2495-501. doi: 10.1002/j.1460-2075.1988.tb03096.x.
Show Gene links
Show Anatomy links
A cell-specific activator in the Xenopus A2 vitellogenin gene: promoter elements functioning with rat liver nuclear extracts.
Döbbeling U
,
Ross K
,
Klein-Hitpass L
,
Morley C
,
Wagner U
,
Ryffel GU
.
???displayArticle.abstract???
Transfection experiments using Xenopus vitellogenin A2 gene constructs allowed us to identify an activator which increases the activity of the thymidine kinase promoter. The activator is located between -121 and -87 of the A2 vitellogenin gene and is separated by a stretch of curved DNA from the estrogen-responsive DNA element at -331. The activator functions in a cell-specific manner, as it is active in human breast cancer cells (MCF-7) as well as hepatoma cells but not in fibroblasts or HeLa cells. The activator is composed of at least three elements: elements 1 and 2 which form a partial palindrome, function independently, but act synergistically when combined. Element 3 is not active on its own, but supports elements 1 and 2. A TATA box region derived from the Xenopus albumin gene is sufficient for the function of the activator. In vitro transcription experiments using rat liver nuclear extracts demonstrate that the activator interacts with transcription factors. These factors are distinct from those recognizing HP1, a regulatory element common to several genes specifically expressed in hepatocytes.
Chen,
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
1985, Pubmed
Chen,
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
1985,
Pubmed
Coon,
A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.
1969,
Pubmed
De Simone,
Cis- and trans-acting elements responsible for the cell-specific expression of the human alpha 1-antitrypsin gene.
1987,
Pubmed
Diekmann,
Sequence specificity of curved DNA.
1986,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Graham,
A new technique for the assay of infectivity of human adenovirus 5 DNA.
1973,
Pubmed
Hsieh,
The terminus of SV40 DNA replication and transcription contains a sharp sequence-directed curve.
1988,
Pubmed
Huber,
Thyroid hormone induces competence for oestrogen-dependent vitellogenin synthesis in developing Xenopus laevis liver.
1979,
Pubmed
,
Xenbase
Jones,
Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
1985,
Pubmed
Klein-Hitpass,
An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.
1986,
Pubmed
,
Xenbase
Klein-Hitpass,
A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor.
1988,
Pubmed
,
Xenbase
Klein-Hitpass,
Synergism of closely adjacent estrogen-responsive elements increases their regulatory potential.
1988,
Pubmed
,
Xenbase
Koo,
DNA bending at adenine . thymine tracts.
,
Pubmed
Kugler,
Tissue-specificity of liver gene expression: a common liver-specific promoter element.
1988,
Pubmed
,
Xenbase
Luckow,
CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements.
1987,
Pubmed
Maniatis,
Regulation of inducible and tissue-specific gene expression.
1987,
Pubmed
Marini,
Bent helical structure in kinetoplast DNA.
1982,
Pubmed
Martinez,
The estrogen-responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid-responsive element.
1987,
Pubmed
,
Xenbase
Mohun,
Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site.
1987,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Satchwell,
Sequence periodicities in chicken nucleosome core DNA.
1986,
Pubmed
Sawadogo,
Energy requirement for specific transcription initiation by the human RNA polymerase II system.
1984,
Pubmed
Schorpp,
5'-flanking and 5'-proximal exon regions of the two Xenopus albumin genes. Deletion analysis of constitutive promoter function.
1988,
Pubmed
,
Xenbase
Seiler-Tuyns,
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
1986,
Pubmed
,
Xenbase
Ulanovsky,
Curved DNA: design, synthesis, and circularization.
1986,
Pubmed
Wahli,
Xenopus vitellogenin genes.
1985,
Pubmed
,
Xenbase
Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase
Wigler,
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
1979,
Pubmed
Wu,
The locus of sequence-directed and protein-induced DNA bending.
,
Pubmed
