Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Different regulatory elements are required for cell-type and stage specific expression of the Xenopus laevis skeletal muscle actin gene upon injection in X.laevis oocytes and embryos.
Steinbeisser H
,
Hofmann A
,
Stutz F
,
Trendelenburg MF
.
???displayArticle.abstract???
In the present study, we demonstrate by transcript mapping that the injected Xenopus skeletal muscle alpha-actin gene is transcribed and spliced in Xenopus oocytes but not correctly initiated at the alpha-actin promoter. This leads to correctly spliced transcripts even if constructs without putative promoter sequences are injected. On the other hand, alpha-actin transcripts are translated in injected oocytes as shown by the detection of alpha-actin protein. By contrast, correctly initiated alpha-actin transcripts can be found in neurula embryos when the injected clone contains 5' flanking sequences extending from +27 to -680. alpha-actin gene fragments without the 680 nucleotides 5' flanking region are activated unspecifically after midblastula transition, whereas the clones carrying this region are activated correctly at the end of gastrulation. Cell type specific expression seems to be modulated by sequences within the transcribed region.
Bachvarova,
Activation of RNA synthesis associated with gastrulation.
1966, Pubmed
Bachvarova,
Activation of RNA synthesis associated with gastrulation.
1966,
Pubmed
Bendig,
Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.
1984,
Pubmed
,
Xenbase
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bozzoni,
Expression of two Xenopus laevis ribosomal protein genes in injected frog oocytes. A specific splicing block interferes with the L1 RNA maturation.
1984,
Pubmed
,
Xenbase
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Etkin,
Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Glisin,
Ribonucleic acid isolated by cesium chloride centrifugation.
1974,
Pubmed
Koltunow,
Intron sequences modulate feather keratin gene transcription in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Koltunow,
Promoter efficiency depends upon intragenic sequences.
1987,
Pubmed
,
Xenbase
Krieg,
Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
1985,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Michaeli,
pBR322 DNA inhibits simian virus 40 gene expression in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Minty,
Upstream regions of the human cardiac actin gene that modulate its transcription in muscle cells: presence of an evolutionarily conserved repeated motif.
1986,
Pubmed
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Mohun,
Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Nellen,
Regulatory sequences in the promoter of the Dictyostelium Actin 6 gene.
1986,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase
Schaufele,
The inability of the Psammechinus miliaris H3 RNA to be processed in the Xenopus oocyte is associated with sequences distinct from those highly conserved amongst sea urchin histone RNAs.
1987,
Pubmed
,
Xenbase
Sollner-Webb,
Accurate transcription of cloned Xenopus rRNA genes by RNA polymerase I: demonstration by S1 nuclease mapping.
1982,
Pubmed
,
Xenbase
Sturgess,
Actin synthesis during the early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Stutz,
Isolation and characterization of sarcomeric actin genes expressed in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Trendelenburg,
Transcription units of chicken ovalbumin gene observed after injection of cloned complete genes into Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Trendelenburg,
DNA injections into Xenopus embryos: fate of injected DNA in relation to formation of embryonic nuclei.
1986,
Pubmed
,
Xenbase
Vandekerckhove,
Chordate muscle actins differ distinctly from invertebrate muscle actins. The evolution of the different vertebrate muscle actins.
1984,
Pubmed
Whalen,
Protein synthesis and actin heterogeneity in calf muscle cells in culture.
1976,
Pubmed
Wilson,
Tissue-specific expression of actin genes injected into Xenopus embryos.
1986,
Pubmed
,
Xenbase
