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Gene Expr Patterns
2004 Nov 01;51:135-40. doi: 10.1016/j.modgep.2004.05.002.
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Identification and characterisation of the posteriorly-expressed Xenopus neurotrophin receptor homolog genes fullback and fullback-like.
Bromley E
,
Knapp D
,
Wardle FC
,
Sun BI
,
Collins-Racie L
,
LaVallie E
,
Smith JC
,
Sive HL
.
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We have identified fullback and fullback-like, two Xenopus laevis neurotrophin receptor homolog (NRH1) genes. The sequences of Fullback and Fullback-like are very similar to that of the neurotrophin receptor p75NTR, in both their extracellular and their intracellular domains. As their names imply, fullback and fullback-like are expressed in essentially identical patterns in the posterior of the embryo from the early gastrula stage onward. At tailbud and tadpole stages transcripts are also present in dorsal somites and the head, in addition to the growing tailbud. This expression pattern differs from that of p75NTR, suggesting that fullback and fullback-like have different functions from p75NTR during early development.
Fig. 3. In situ hybridization analysis of fullback RNA expression at gastrula and neurula stages. In situ hybridization was performed on intact (A,C,E,H) or bisected (B,D,F,G) albino Xenopus embryos. Dashed lines in panel (E) indicates the plane in which the embryo in (F) was cut in half. (A) Vegetal view of an early gastrula (stage 10). (B) Bisected embryo at early gastrula stage 10. (C) Posterior view of an embryo at mid-gastrula stage 11.5. (D) Embryo bisected sagittally at mid-gastrula stage 11.5. Note staining in involuted mesoderm. (E) Dorsal view of an embryo at early neurula stage 13. Arrows indicates stripes of staining on the dorsal side and dashed line indicates plane of section of embryo in (F). (F) Bisected embryo at early neurula stage 13. (G) Embryos bisected sagittally at early neurula stage 13. Note posterior staining. (H) Dorsal view of an embryo at late neurula stage 18. For all images, Vg, vegetal; D, dorsal; V, ventral; A, anterior; P, posterior.
Fig. 4. In situ hybridization analysis of fullback and muscle-specific actin at neurula and tailbud stages. (A,B) Embryos at late neurula stage 18 were bisected transversely and the resulting halves were probed with a fullback (A) or muscle-specific actin (B) probe. Note that the expression domains of fullback and muscle-specific actin overlap to a very limited extent in the dorsal region of the embryo. (C). Intact embryo at stage 24 showing expression of fullback in somites and in a stream of cells which derive from the hindbrain (arrowhead). (D,E) Embryos at stage 25 were bisected transversely and the resulting halves were probed with a fullback (D) or muscle-specific actin (E) probe. Note that the expression domains of fullback and muscle-specific actin overlap in the dorsal regions of the somites. (F) Embryo at stage 35. Note the expression of fullback in the cranial nerves (white arrows), branchial arches (black arrows), diencephalon (d) and midbrain (m). Similar expression patterns to those observed in Fig. 3 and Fig. 4 were obtained with fullback-like antisense probe and with probes derived from the 3′ untranslated regions of fullback and fullback-like.
ngfr (nerve growth factor receptor) gene expression in bisected Xenopus laevis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 11.5, dorsal left, ventralright, anterior up.
ngfr (nerve growth factor receptor) gene expression in bisected Xenopus laevis embryo, assayed via in situ hybridization, NF stage 17/18, dorsal view, anteriorleft.
ngfr (nerve growth factor receptor) gene expression in bisected Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, lateral view, anteriorleft, dorsal up.
