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Adenovirus E1A requires synthesis of a cellular protein to establish a stable transcription complex in injected Xenopus laevis oocytes.
Richter JD
,
Hurst HC
,
Jones NC
.
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The Escherichia coli-expressed adenovirus E1A 13S mRNA product injected into Xenopus oocytes was active, as assessed by its ability to stimulate the transcription of an injected gene which is normally responsive to E1A in mammalian cells. In the presence of the protein synthesis inhibitors pactamycin or cycloheximide, E1A was correctly posttranslationally modified (phosphorylated) and transported to the nucleus; but it failed to stimulate the transcription of an injected gene containing the human heat shock protein 70 promoter. The basal (unstimulated) level of transcription of the gene was unaffected by these inhibitors. If oocytes were cultured in the presence of cycloheximide after E1A stimulated transcription, however, the high level of transcription was maintained for several hours without new protein synthesis. Results of competition studies with the same promoter (the heat shock protein 70 promoter) linked to two marked genes demonstrated that once the induction of transcription by E1A took place, the stimulated levels of transcription were maintained, even when they were challenged with excess competitor DNA. Results of these studies suggest that E1A requires the synthesis of a cellular protein to form a stable transcription complex.
Berk,
Adenovirus promoters and E1A transactivation.
1986, Pubmed
Berk,
Adenovirus promoters and E1A transactivation.
1986,
Pubmed
Berk,
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
1979,
Pubmed
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Borrelli,
Adenovirus-2 E1A products repress enhancer-induced stimulation of transcription.
,
Pubmed
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase
Colbere-Garapin,
Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
1979,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Ferguson,
Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional.
1984,
Pubmed
,
Xenbase
Ferguson,
E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
1985,
Pubmed
Gaynor,
Cis-acting induction of adenovirus transcription.
1983,
Pubmed
Harlow,
Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.
1985,
Pubmed
Harlow,
Association of adenovirus early-region 1A proteins with cellular polypeptides.
1986,
Pubmed
Imperiale,
Adenovirus 5 E2 transcription unit: an E1A-inducible promoter with an essential element that functions independently of position or orientation.
1984,
Pubmed
Imperiale,
An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription.
1985,
Pubmed
Jones,
An adenovirus type 5 early gene function regulates expression of other early viral genes.
1979,
Pubmed
Jones,
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Kao,
Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection.
1983,
Pubmed
King,
Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting.
1985,
Pubmed
Kingston,
Regulation of transcription of the adenovirus EII promoter by EIa gene products: absence of sequence specificity.
1984,
Pubmed
Kovesdi,
E1A transcription induction: enhanced binding of a factor to upstream promoter sequences.
1986,
Pubmed
Kovesdi,
Identification of a cellular transcription factor involved in E1A trans-activation.
1986,
Pubmed
Krippl,
Functions of purified E1A protein microinjected into mammalian cells.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Leff,
Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products.
1985,
Pubmed
Murthy,
Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent.
1985,
Pubmed
Nevins,
Mechanism of activation of early viral transcription by the adenovirus E1A gene product.
1981,
Pubmed
Nevins,
Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.
1982,
Pubmed
Richter,
A first exon-encoded domain of E1A sufficient for posttranslational modification, nuclear-localization, and induction of adenovirus E3 promoter expression in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
SivaRaman,
Identification of a factor in HeLa cells specific for an upstream transcriptional control sequence of an EIA-inducible adenovirus promoter and its relative abundance in infected and uninfected cells.
1986,
Pubmed
Spindler,
Rapid intracellular turnover of adenovirus 5 early region 1A proteins.
1984,
Pubmed
Stein,
HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism.
1984,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Velcich,
Adenovirus E1a proteins repress transcription from the SV40 early promoter.
1985,
Pubmed
Weeks,
E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.
1983,
Pubmed
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Wu,
Structure and expression of the human gene encoding major heat shock protein HSP70.
1985,
Pubmed
Wu,
Human HSP70 promoter contains at least two distinct regulatory domains.
1986,
Pubmed
Wu,
The E1A 13S product of adenovirus 5 activates transcription of the cellular human HSP70 gene.
1986,
Pubmed
Yee,
Detection of cellular proteins associated with human adenovirus type 5 early region 1A polypeptides.
1985,
Pubmed
