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Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
Krol A
,
Carbon P
,
Ebel JP
,
Appel B
.
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We have cloned and sequenced a 977bp DNA fragment, pXTU6-2, that represents the transcription unit for a Xenopus tropicalis U6 RNA gene. This basic repeating unit is reiterated ca.500-fold per haploid genome. Oocyte injections of pXTU6-2 led to the transcription of a mature-sized U6 RNA that, however, lacked internal 2'-O-methylations. These posttranscriptional modifications of U6 RNA might be cytoplasmic and could require its association with U4 RNA to be accomplished. The low alpha- amanitin sensitivity of U6 RNA synthesis in oocytes suggested that U6 RNA is transcribed by RNA polymerase III, consistent with features of the U6 RNA molecule which also contains a Box A- like intragenic control region. Inspection of X. tropicalis, mouse and human U6 DNA upstream sequences revealed the presence of a TATA box as well as of the proximal and enhancer (octamer motif) elements contained in snRNA genes transcribed by RNA polymerase II. We propose that U6 RNAs are synthesized by a specialized transcription complex consisting of RNA polymerase III and transcription factors, some of which are very likely shared with RNA polymerase II promoters.
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986, Pubmed
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986,
Pubmed
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986,
Pubmed
Black,
Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.
1986,
Pubmed
Branlant,
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
1982,
Pubmed
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Bringmann,
Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.
1984,
Pubmed
Chandrasekharappa,
Biosynthesis of small nuclear RNAs in human cells.
1983,
Pubmed
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Ciliberto,
Transcription signals in embryonic Xenopus laevis U1 RNA genes.
1985,
Pubmed
,
Xenbase
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Harada,
The nucleotide sequence of nuclear 4.8S RNA of mouse cells.
1980,
Pubmed
Hashimoto,
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
1984,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.
1986,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed
Hoffman,
Structural and functional analysis of chicken U4 small nuclear RNA genes.
1986,
Pubmed
,
Xenbase
Krol,
The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
1985,
Pubmed
,
Xenbase
Kunkel,
U6 small nuclear RNA is transcribed by RNA polymerase III.
1986,
Pubmed
Larson,
A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.
1983,
Pubmed
Lund,
The two embryonic U1 small nuclear RNAs of Xenopus laevis are encoded by a major family of tandemly repeated genes.
1984,
Pubmed
,
Xenbase
Mangin,
Human U2 small nuclear RNA genes contain an upstream enhancer.
1986,
Pubmed
,
Xenbase
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase
Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Ohshima,
Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.
1981,
Pubmed
Reddy,
The capped U6 small nuclear RNA is transcribed by RNA polymerase III.
1987,
Pubmed
,
Xenbase
Rinke,
Association of the lupus antigen La with a subset of U6 snRNA molecules.
1985,
Pubmed
Rinke,
Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking.
1985,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Savouret,
Interaction of small nuclear ribonucleoproteins with simian virus 40 in CV-1 cells: is U2 snRNA involved in regulating replication?
1984,
Pubmed
Selker,
An upstream signal is required for in vitro transcription of Neurospora 5S RNA genes.
1986,
Pubmed
Singh,
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
,
Pubmed
Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
,
Xenbase
Smith,
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
1980,
Pubmed
Theissen,
Novel structure of a human U6 snRNA pseudogene.
1985,
Pubmed
Ullu,
Upstream sequences modulate the internal promoter of the human 7SL RNA gene.
,
Pubmed
Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase
Zenke,
Multiple sequence motifs are involved in SV40 enhancer function.
1986,
Pubmed
de Vegvar,
3' end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters.
1986,
Pubmed
