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XB-ART-28429
Nucleic Acids Res 1986 Dec 09;1423:9329-37.
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Introduction of estrogen-responsiveness into mammalian cell lines.

Druege PM , Klein-Hitpass L , Green S , Stack G , Chambon P , Ryffel GU .


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We introduced estrogen responsiveness as a new characteristic into rat hepatoma, mouse Ltk- and human HeLatk-cells by transfecting the human estrogen receptor (ER) cDNA. To measure the estrogen response we used Xenopus vitellogenin gene A2 constructs linked to the bacterial CAT gene. Transient cotransfections of the ER cDNA and the vitellogenin gene-CAT constructs containing the estrogen responsive element (ERE) lead to a hormone dependent induction of CAT activity whereas cotransfected vitellogenin gene constructs lacking the ERE are not inducible. Stable transfections of ER cDNA into Ltk- cells give rise to cell clones that are estrogen responsive as shown by transfection of various vitellogenin gene-CAT constructs. These results prove that the transfected ER is biologically active and is sufficient to make a cell estrogen responsive.

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Species referenced: Xenopus
Genes referenced: ltk

References [+] :
Banerji, A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. 1983, Pubmed