Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1986 Dec 01;512:3305-11. doi: 10.1002/j.1460-2075.1986.tb04644.x.
Show Gene links
Show Anatomy links
Novobiocin inhibits passive chromatin assembly in vitro.
Sealy L
,
Cotten M
,
Chalkley R
.
???displayArticle.abstract???
Novobiocin, an inhibitor of prokaryotic DNA gyrase and eukaryotic type II topoisomerase enzymes, interferes with in vitro chromatin assembly using purified histones, DNA and nucleoplasmin. The target of inhibition is not topoisomerase II; this energy-independent assembly system lacks any ATP and Mg2+-dependent type II topoisomerase or gyrase activities. Rather, novobiocin interacts with histones, disrupting histone-histone associations required for octamer formation, and causing histones to precipitate from both nucleoplasmin-histone and histone-DNA complexes. Thus, novobiocin is able to generate 'dynamic' chromatin in vitro in the absence of ATP and Mg2+ by removing histones from previously assembled static chromatin, so that the DNA supercoils, previously constrained by conventional nucleosomes, become susceptible to removal by topoisomerase I.
Annunziato,
Chromatin replication, reconstitution and assembly.
1983, Pubmed
Annunziato,
Chromatin replication, reconstitution and assembly.
1983,
Pubmed
Cotten,
Hyperacetylated histones facilitate chromatin assembly in vitro.
1985,
Pubmed
Cotten,
Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomerase.
1986,
Pubmed
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Gellert,
Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
1976,
Pubmed
Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hsieh,
ATP-dependent DNA topoisonmerase from D. melanogaster reversibly catenates duplex DNA rings.
1980,
Pubmed
Isenberg,
Histones.
1979,
Pubmed
Jackson,
Studies on histone organization in the nucleosome using formaldehyde as a reversible cross-linking agent.
1978,
Pubmed
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase
Krohne,
Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus.
1980,
Pubmed
,
Xenbase
Krohne,
A major soluble acidic protein located in nuclei of diverse vertebrate species.
1980,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Osheroff,
DNA topoisomerase II from Drosophila melanogaster. Relaxation of supercoiled DNA.
1983,
Pubmed
Richmond,
Structure of the nucleosome core particle at 7 A resolution.
,
Pubmed
Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Ryoji,
Structure of the two distinct types of minichromosomes that are assembled on DNA injected in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Sealy,
Xenopus nucleoplasmin: egg vs. oocyte.
1986,
Pubmed
,
Xenbase
Sealy,
Site-specific mutagenesis of avian erythroblastosis virus: erb-B is required for oncogenicity.
1983,
Pubmed
Shure,
The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.
1976,
Pubmed
Sinden,
Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells.
1980,
Pubmed
Stein,
Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.
1979,
Pubmed
Sugino,
Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
1978,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Wray,
Silver staining of proteins in polyacrylamide gels.
1981,
Pubmed
