Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Activation of ribosomal protein S6 phosphorylation during meiotic maturation of Xenopus laevis oocytes: in vitro ordered appearance of S6 phosphopeptides.
Martin-Pérez J
,
Rudkin BB
,
Siegmann M
,
Thomas G
.
???displayArticle.abstract???
During meiotic maturation of Xenopus laevis stage 6 oocytes into unfertilized eggs, 40S ribosomal protein S6 undergoes multiple phosphorylation. Extracts prepared from unfertilized eggs are up to 10-fold more efficient in phosphorylating S6 than those prepared from immature oocytes. When analyzed by DEAE chromatography the S6 kinase activity elutes as a single peak. If extracts from unfertilized eggs are prepared in the absence of beta-glycerol phosphate, a putative phosphatase inhibitor, there is a severe reduction in recovered S6 kinase activity. Under optimal conditions, incubation of unfertilized egg extracts with 40S ribosomes in the presence of ATP leads to the average incorporation of 3.5 mol of phosphate/mol of S6. Prior incubation of these extracts with the cAMP-dependent protein kinase inhibitor does not inhibit S6 phosphorylation indicating that another kinase is responsible. Analysis of the in vitro phosphorylated peptides demonstrates that they migrate to the equivalent position of those observed previously in vivo and in vitro. More strikingly, if each of the increasingly phosphorylated derivatives of S6 is analyzed independently, it is found that the phosphopeptides appear in a specific order.
Ballinger,
Fertilization of sea urchin eggs is accompanied by 40 S ribosomal subunit phosphorylation.
1981, Pubmed
Ballinger,
Fertilization of sea urchin eggs is accompanied by 40 S ribosomal subunit phosphorylation.
1981,
Pubmed
Blenis,
Phorbol ester, serum, and rous sarcoma virus transforming gene product induce similar phosphorylations of ribosomal protein S6.
1984,
Pubmed
Bommer,
Immunochemical detection of proteins in the small subunit of rat liver ribosomes involved in binding of the ternary initiation complex.
1980,
Pubmed
Burkhard,
Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6.
1983,
Pubmed
Cobb,
Description of a protein kinase derived from insulin-treated 3T3-L1 cells that catalyzes the phosphorylation of ribosomal protein S6 and casein.
1983,
Pubmed
Decker,
Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
1981,
Pubmed
Demaille,
Isolation and properties of the rabbit skeletal muscle protein inhibitor of adenosine 3',5'-monophosphate dependent protein kinases.
1977,
Pubmed
Duncan,
Preferential utilization of phosphorylated 40-S ribosomal subunits during initiation complex formation.
1982,
Pubmed
Erikson,
A protein kinase from Xenopus eggs specific for ribosomal protein S6.
1985,
Pubmed
,
Xenbase
Gressner,
Interaction of synthetic polynucleotides with small rat liver ribosomal subunits possessing low and highly phosphorylated protein S6.
1980,
Pubmed
Gressner,
The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated.
1974,
Pubmed
Hallberg,
Regulation of ribosome phosphorylation and antibiotic sensitivity in Tetrahymena thermophila: A correlation.
1981,
Pubmed
Hanocq-Quertier,
Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Haselbacher,
Insulin-like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G1 phase of the cell cycle.
1979,
Pubmed
Kisilevsky,
Ribosome conformational changes associated with protein S6 phosphorylation.
1984,
Pubmed
Kruppa,
The phosphorylation of ribosomal protein S6 from progesterone-stimulated Xenopus laevis oocytes. Kinetic studies and phosphopeptide analysis.
1983,
Pubmed
,
Xenbase
Lubben,
Cyclic nucleotide-independent protein kinases from rabbit reticulocytes. Purification and characterization of protease-activated kinase II.
1983,
Pubmed
Maller,
Phosphorylation of ribosomal protein S6 on serine after microinjection of the Abelson murine leukemia virus tyrosine-specific protein kinase into Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Martin-Pérez,
Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells.
1983,
Pubmed
Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Nielsen,
The phosphorylation of ribosomal protein S6 in rat tissues following cycloheximide injection, in diabetes, and after denervation of diaphragm. A simple immunological determination of the extent of S6 phosphorylation on protein blots.
1982,
Pubmed
Novak-Hofer,
Epidermal growth factor-mediated activation of an S6 kinase in Swiss mouse 3T3 cells.
1985,
Pubmed
Novak-Hofer,
An activated S6 kinase in extracts from serum- and epidermal growth factor-stimulated Swiss 3T3 cells.
1984,
Pubmed
Parker,
The phosphorylation of eukaryotic ribosomal protein S6 by protein kinase C.
1985,
Pubmed
Ramjoué,
Evolutionary microdivergence of chick and rat liver ribosomal proteins.
1977,
Pubmed
Tabarini,
Activation of S6 kinase activity in 3T3-L1 cells by insulin and phorbol ester.
1985,
Pubmed
Terao,
Proteins of small subunits of rat liver ribosomes that interact with poly(U). I. Effects of preincubation of poly(U) with 40 S subunits on the interactions of 40 S subunit proteins with aurintricarboxylic acid and with N,N'-p-phenylenedimaleimide.
1979,
Pubmed
Terao,
Proteins of small subunits of rat liver ribosomes that interact with poly(U). II. Cross-links between poly(U) and ribosomal proteins in 40 S subunits induced by UV irradiation.
1979,
Pubmed
Thomas,
N4-Acetylcytidine. A previously unidentified labile component of the small subunit of eukaryotic ribosomes.
1978,
Pubmed
Thomas,
Regulation of 40S ribosomal protein S6 phosphorylation in Swiss mouse 3T3 cells.
1980,
Pubmed
Thomas,
The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis.
1982,
Pubmed
Tolan,
Protein topography of the 40 S ribosomal subunit from rabbit reticulocytes shown by cross-linking with 2-iminothiolane.
1981,
Pubmed
Wettenhall,
Isolation and characterisation of cyclic AMP-dependent phosphorylation sites from rat liver ribosomal protein S6.
1982,
Pubmed
Wettenhall,
Phosphorylation sites for ribosomal S6 protein kinases in mouse 3T3 fibroblasts stimulated with platelet-derived growth factor.
1983,
Pubmed
Wu,
Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
del Grande,
Phosphorylation of 40-S ribosomal subunits by cAMP-dependent, cGMP-dependent and protease-activated protein kinases.
1982,
Pubmed
