Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Functional analysis of a herpes simplex virus type 1 promoter: identification of far-upstream regulatory sequences.
Cordingley MG
,
Campbell ME
,
Preston CM
.
???displayArticle.abstract???
We have performed a functional analysis of DNA sequences upstream from the gene for IE mRNA3 of herpes simplex virus type 1. Nucleotide sequences involved in initiation and positive regulation of transcription have been defined by construction of specific deletions in vitro. Transcription was assayed in vivo by microinjection into Xenopus oocytes, or by introduction of plasmid DNA into tissue culture cells and measurement of transient expression. Three functional promoter elements have been defined: i) Sequences between -16 and -37 which are not essential for transcription but are required for accurate initiation. ii) Proximal promoter sequences which are sufficient for transcription initiation in the absence of upstream sequences. iii) Far-upstream promoter sequences (more than 108bp upstream) which increase transcription in oocytes, and contain positive regulatory sequences (-174 to -331) which respond strongly to a factor in the virus inoculum.
Banerji,
Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
1981, Pubmed
Banerji,
Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
1981,
Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed
Benoist,
In vivo sequence requirements of the SV40 early promotor region.
1981,
Pubmed
Berk,
Spliced early mRNAs of simian virus 40.
1978,
Pubmed
Brinster,
Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs.
1982,
Pubmed
Capecchi,
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.
1980,
Pubmed
Clements,
Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome.
1977,
Pubmed
Cordingley,
Transcription and translation of the herpes simplex virus type 1 thymidine kinase gene after microinjection into Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Costanzo,
Evidence that herpes simplex virus DNA is transcribed by cellular RNA polymerase B.
1977,
Pubmed
Dhar,
Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.
1980,
Pubmed
Graham,
Infectious herpesvirus DNA.
1973,
Pubmed
Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Xenbase
Grosveld,
DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.
1982,
Pubmed
Grosveld,
Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region.
1982,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Jamieson,
Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity.
1974,
Pubmed
Jones,
Regulation of herpesvirus macromolecular synthesis. VIII. The transcription program consists of three phases during which both extent of transcription and accumulation of RNA in the cytoplasm are regulated.
1979,
Pubmed
Kozak,
Regulation of herpesvirus macromolecular synthesis: nuclear retention of nontranslated viral RNA sequences.
1974,
Pubmed
Leiden,
Herpes simplex virus gene expression in transformed cells. I. Regulation of the viral thymidine kinase gene in transformed L cells by products of superinfecting virus.
1976,
Pubmed
Leung,
Herpes simplex virus thymidine kinase transcripts are absent from both nucleus and cytoplasm during infection in the presence of cycloheximide.
1980,
Pubmed
Lilley,
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.
1980,
Pubmed
Mackem,
Regulation of alpha genes of herpes simplex virus: the alpha 27 gene promoter-thymidine kinase chimera is positively regulated in converted L cells.
1982,
Pubmed
Mackem,
Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.
1980,
Pubmed
Mackem,
Differentiation between alpha promoter and regulator regions of herpes simplex virus 1: the functional domains and sequence of a movable alpha regulator.
1982,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKnight,
Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
1981,
Pubmed
,
Xenbase
McKnight,
Transcriptional control signals of a eukaryotic protein-coding gene.
1982,
Pubmed
Mellon,
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
1981,
Pubmed
Murchie,
DNA sequence analysis of an immediate-early gene region of the herpes simplex virus type 1 genome (map coordinates 0.950 to 0.978).
1982,
Pubmed
Notarianni,
Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides.
1982,
Pubmed
Panayotatos,
Cruciform structures in supercoiled DNA.
1981,
Pubmed
Pelham,
A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
1982,
Pubmed
Pellicer,
Altering genotype and phenotype by DNA-mediated gene transfer.
1980,
Pubmed
Post,
Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.
1981,
Pubmed
Preston,
The cell-free synthesis of herpesvirus-induced polypeptides.
1977,
Pubmed
Preston,
Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK.
1979,
Pubmed
Proudfoot,
Eukaryotic promoters?
1979,
Pubmed
Rixon,
The immediate-early mRNA that encodes the regulatory polypeptide Vmw 175 of herpes simplex virus type 1 is unspliced.
1982,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Stow,
An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.
1976,
Pubmed
Swanstrom,
Regulation of synthesis of herpes simplex type 1 virus mRNA during productive infection.
1974,
Pubmed
Szybalska,
Genetics of human cell line. IV. DNA-mediated heritable transformation of a biochemical trait.
1962,
Pubmed
Twigg,
Trans-complementable copy-number mutants of plasmid ColE1.
1980,
Pubmed
Watson,
A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis.
1980,
Pubmed
Watson,
DNA sequence of an immediate-early gene (IEmRNA-5) of herpes simplex virus type I.
1982,
Pubmed
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Wilkie,
Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1.
1979,
Pubmed
Zipser,
Mapping functional domains in the promoter region of the herpes thymidine kinase gene.
1981,
Pubmed
de Villiers,
A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
1981,
Pubmed
