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XB-ART-31703
Cell Tissue Res May 25, 1979; 198 (3): 397-409.

Morphological and physiological aspects of melanophores in primary culture from tadpoles of Xenopus laevis.

Seldenrijk R , Hup DR , de Graan PN , van de Veerdonk FC .


Abstract
Melanophores from tadpoles of Xenopus laevis (Daudin) were isolated by digestion of tail fins with acetyltrypsin and collagenase and maintained in primary culture for 6 weeks up to 3 months. Within 36 to 72 h the melanophores develop one to eight dendritic processes per cell; secondary and tertiary branchings of the processes were frequently observed. The melanophores in primary culture disperse under the influence of alpha-MSH or cyclic AMP; upon rinsing out these substances the cells aggregate. In darkness, about 40% of the cells disperse their pigment, whereas under illumination the pigment of the melanophores aggregates. To date, attempts to initiate cell division in melanophores have not been successful.

PubMed ID: 223762
Article link:



References [+] :
Abe, Adenosine 3',5'-monophosphate (cyclic AMP) as the mediator of the actions of melanocyte stimulating hormone (MSH) and norepinephrine on the frog skin. 1969, Pubmed