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Science March 26, 1976; 191 (4233): 1266-8.
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A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.

Wasserman WJ , Masui Y .

Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.

PubMed ID: 1083070
Article link: Science