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Chemical composition of an oestrogen-induced calcium-binding glycolipophosphoprotein in Xenopus laevis.
Ansari AQ
,
Dolphin PJ
,
Lazier CB
,
Munday KA
,
Akhtar M
.
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1. Oestrogen treatment has previously been shown to induce the formation of large amounts of a serum protein, vitellogenin (xenoprotein), in Xenopus laevis. Vitellogenin was purified from serum by dimethylformamide precipitation and was shown to be homogeneous by a variety of electrophoretic techniques. 2. The molecular weight of vitellogenin was estimated by gel filtration to be about 6x10(5). The chemical constituents of vitellogenin were determined and lead to the characterization of this protein as a serum calcium-binding glycolipophosphoprotein. 3. The extractable lipid accounted for 12% of vitellogenin. Gas-liquid-chromatographic analysis of the saponified lipid moiety showed the presence of palmitic acid, palmitoleic acid, stearic acid, oleic acid and linoleic acid in the molecular proportions 6.8: 1.5: 1.0: 3.6: 1.4. 4. The carbohydrate moiety consisted of 0.4g of hexose, 0.77g of hexosamine and 0.18g of sialic acid/100g of vitellogenin. 5. The calcium and phosphorus contents were 0.85 and 1.65g/100g of vitellogenin respectively. 6. Serum from oestrogen-treated animals injected with (45)CaCl(2) contained 9.7 times the radioactivity present in serum from untreated (45)CaCl(2)-injected animals. Of the radioactivity due to (45)CaCl(2) in the serum of oestrogen-treated animals 72% was non-diffusible on dialysis. Of this activity 65.4% was associated with the vitellogenin band on cellulose acetate electrophoresis.
CLEGG,
Electrophoretic comparison of the serum proteins of normal and diethyl-stilbestrol-treated cockerels.
1951, Pubmed
CLEGG,
Electrophoretic comparison of the serum proteins of normal and diethyl-stilbestrol-treated cockerels.
1951,
Pubmed
DeGuzman,
The chemical nature of component I, a phosphoprotein isolated from the blood sera of diethylstillbestrol-treated cockerels.
1968,
Pubmed
Follett,
The effects of oestrogen and gonadotrophins on lipid and protein metabolism in Xenopus laevis Daudin.
1968,
Pubmed
,
Xenbase
HEALD,
THE SYNTHESIS OF PHOSVITIN IN VITRO BY SLICES OF LIVER FROM THE LAYING HEN.
1965,
Pubmed
Heald,
The isolation of phosvitin from the plasma of the oestrogen-treated immature pullet.
1964,
Pubmed
JOUBERT,
Preparation and characterization of phosvitin from hen egg yolk.
1958,
Pubmed
MOK,
A comparison of phosvitins prepared from hen's serum and from hen's egg yolk.
1961,
Pubmed
Munday,
Oestrogen-induced calcium-binding protein in Xenopus laevis.
1968,
Pubmed
,
Xenbase
Pan,
Vitellogenic blood protein synthesis by insect fat body.
1969,
Pubmed
RONDLE,
The determination of glucosamine and galactosamine.
1955,
Pubmed
Rudack,
On the site of phosvitin synthesis in Xenopus laevis.
1968,
Pubmed
,
Xenbase
SCHJEIDE,
Proteins and calcium in egg yolk.
1959,
Pubmed
SVENNERHOLM,
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
1957,
Pubmed
Swenson,
The synthesis and secretion of gamma-globulin by lymph node cells. 3. The slow acquisition of the carbohydrate moiety of gamma-globulin and its relationship to secretion.
1968,
Pubmed
WALLACE,
STUDIES ON AMPHIBIAN YOLK. III. A RESOLUTION OF YOLK PLATELET COMPONENTS.
1963,
Pubmed
WEIL-MALHERBE,
The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds.
1951,
Pubmed
WINZLER,
Determination of serum glycoproteins.
1955,
Pubmed
Wallace,
Studies on amphibian yolk. IX. Xenopus vitellogenin.
1970,
Pubmed
,
Xenbase
Wallace,
Studies on amphibian yolk. 8. The estrogen-induced hepatic synthesis of a serum lipophosphoprotein and its selective uptake by the ovary and trasformation into yolk platelet proteins in Xenopus laevis.
1969,
Pubmed
,
Xenbase
