Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
2006 Dec 01;9111:4054-63. doi: 10.1529/biophysj.106.085787.
Show Gene links
Show Anatomy links
Effect of charge substitutions at residue his-142 on voltage gating of connexin43 channels.
Shibayama J
,
Gutiérrez C
,
González D
,
Kieken F
,
Seki A
,
Carrión JR
,
Sorgen PL
,
Taffet SM
,
Barrio LC
,
Delmar M
.
???displayArticle.abstract???
Previous studies indicate that the carboxyl terminal of connexin43 (Cx43CT) is involved in fast transjunctional voltage gating. Separate studies support the notion of an intramolecular association between Cx43CT and a region of the cytoplasmic loop (amino acids 119-144; referred to as "L2"). Structural analysis of L2 shows two alpha-helical domains, each with a histidine residue in its sequence (H126 and H142). Here, we determined the effect of H142 replacement by lysine, alanine, and glutamate on the voltage gating of Cx43 channels. Mutation H142E led to a significant reduction in the frequency of occurrence of the residual state and a prolongation of dwell open time. Macroscopically, there was a large reduction in the fast component of voltage gating. These results resembled those observed for a mutant lacking the carboxyl terminal (CT) domain. NMR experiments showed that mutation H142E significantly decreased the Cx43CT-L2 interaction and disrupted the secondary structure of L2. Overall, our data support the hypothesis that fast voltage gating involves an intramolecular particle-receptor interaction between CT and L2. Some of the structural constrains of fast voltage gating may be shared with those involved in the chemical gating of Cx43.
Anumonwo,
The carboxyl terminal domain regulates the unitary conductance and voltage dependence of connexin40 gap junction channels.
2001, Pubmed,
Xenbase
Anumonwo,
The carboxyl terminal domain regulates the unitary conductance and voltage dependence of connexin40 gap junction channels.
2001,
Pubmed
,
Xenbase
Banach,
Connexin43 gap junctions exhibit asymmetrical gating properties.
1996,
Pubmed
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bukauskas,
Conductance and permeability of the residual state of connexin43 gap junction channels.
2002,
Pubmed
Bukauskas,
Gating properties of gap junction channels assembled from connexin43 and connexin43 fused with green fluorescent protein.
2001,
Pubmed
Duffy,
pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains.
2002,
Pubmed
Homma,
A particle-receptor model for the insulin-induced closure of connexin43 channels.
1998,
Pubmed
,
Xenbase
Kambouris,
Phenotypic characterization of a novel long-QT syndrome mutation (R1623Q) in the cardiac sodium channel.
1998,
Pubmed
,
Xenbase
Krueger-Koplin,
An evaluation of detergents for NMR structural studies of membrane proteins.
2004,
Pubmed
Martin,
Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments.
1998,
Pubmed
,
Xenbase
Moreno,
Gap junction channels: distinct voltage-sensitive and -insensitive conductance states.
1994,
Pubmed
Moreno,
Role of the carboxyl terminal of connexin43 in transjunctional fast voltage gating.
2002,
Pubmed
,
Xenbase
Morley,
Intramolecular interactions mediate pH regulation of connexin43 channels.
1996,
Pubmed
,
Xenbase
Purnick,
Structure of the amino terminus of a gap junction protein.
2000,
Pubmed
,
Xenbase
Revilla,
Molecular dissection of transjunctional voltage dependence in the connexin-32 and connexin-43 junctions.
1999,
Pubmed
,
Xenbase
Ri,
The role of a conserved proline residue in mediating conformational changes associated with voltage gating of Cx32 gap junctions.
1999,
Pubmed
,
Xenbase
Seki,
Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region.
2004,
Pubmed
Seki,
Loss of electrical communication, but not plaque formation, after mutations in the cytoplasmic loop of connexin43.
2004,
Pubmed
Shibayama,
Identification of a novel peptide that interferes with the chemical regulation of connexin43.
2006,
Pubmed
Sorgen,
Sequence-specific resonance assignment of the carboxyl terminal domain of Connexin43.
2002,
Pubmed
Sorgen,
Structure of the Rhodobacter sphaeroides light-harvesting 1 beta subunit in detergent micelles.
2002,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
