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Differentiation March 1, 2007; 75 (3): 235-45.

Xenopus Tetraspanin-1 regulates gastrulation movements and neural differentiation in the early Xenopus embryo.

Yamamoto Y , Grubisic K , Oelgeschläger M .

The tetraspanin family of four-pass transmembrane proteins has been implicated in fundamental biological processes, including cell adhesion, migration, and proliferation. Tetraspanins interact with various transmembrane proteins, establishing a network of large multimolecular complexes that allows specific lateral secondary interactions. Here we report the identification and functional characterization of Xenopus Tetraspanin-1 (xTspan-1). At gastrula and neurula, xTspan-1 is expressed in the dorsal ectoderm and neural plate, respectively, and in the hatching gland, cement gland, and posterior neural tube at tailbud stages. The expression of xTspan-1 in the early embryo is negatively regulated by bone morphogenetic protein (BMP) and stimulated by Notch signals. Microinjection of xTspan-1 mRNA interfered with gastrulation movements and reduced ectodermal cell adhesion in a cadherin-dependent manner. Morpholino knock-down of endogenous xTspan-1 protein revealed a requirement of xTspan-1 for gastrulation movements and primary neurogenesis. Our data suggest that xTspan-1 could act as a molecular link between BMP signalling and the regulation of cellular interactions that are required for gastrulation movements and neural differentiation in the early Xenopus embryo.

PubMed ID: 17359299
Article link: Differentiation

Species referenced: Xenopus laevis
Genes referenced: chrd.1 myod1 notch1 odc1 rax shh szl tbxt tspan1
GO keywords: gastrulation [+]
Morpholinos: tspan1 MO1 tspan1 MO2

Article Images: [+] show captions