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Biochemistry
2007 Feb 27;468:2091-9. doi: 10.1021/bi602366n.
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Nucleosome recognition by the Piccolo NuA4 histone acetyltransferase complex.
Berndsen CE
,
Selleck W
,
McBryant SJ
,
Hansen JC
,
Tan S
,
Denu JM
.
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The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of nucleosome recognition by the MOZ, Ybf2/Sas3, Sas2, Tip60 family HAT complex, Piccolo NuA4 (picNuA4). We find the histone tails to be dispensable for binding to both nucleosomes and free histones and that the H2A, H3, and H2B tails do not influence the ability of picNuA4 to tetra-acetylate the H4 tail within the nucleosome. Most notably, we discovered that the histone-fold domain (HFD) regions of histones, particularly residues 21-52 of H4, are critical for tight binding and efficient tail acetylation. Presented evidence suggests that picNuA4 recognizes the open surface of the nucleosome on which the HFD of H4 is located. This binding mechanism serves to direct substrate access to the tails of H4 and H2A and allows the enzyme to be "tethered", thereby increasing the effective concentration of the histone tail and permitting successive cycles of H4 tail acetylation.
ALLFREY,
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
1964, Pubmed
ALLFREY,
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
1964,
Pubmed
Akhtar,
The histone H4 acetyltransferase MOF uses a C2HC zinc finger for substrate recognition.
2001,
Pubmed
,
Xenbase
Allard,
NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p.
1999,
Pubmed
Barron,
Serum response factor, an enriched cardiac mesoderm obligatory factor, is a downstream gene target for Tbx genes.
2005,
Pubmed
Berndsen,
Assays for mechanistic investigations of protein/histone acetyltransferases.
2005,
Pubmed
Bickmore,
Perturbations of chromatin structure in human genetic disease: recent advances.
2003,
Pubmed
Bird,
Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair.
2002,
Pubmed
Boudreault,
Yeast enhancer of polycomb defines global Esa1-dependent acetylation of chromatin.
2003,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Carrozza,
The diverse functions of histone acetyltransferase complexes.
2003,
Pubmed
Carruthers,
Assembly of defined nucleosomal and chromatin arrays from pure components.
1999,
Pubmed
Clarke,
Esa1p is an essential histone acetyltransferase required for cell cycle progression.
1999,
Pubmed
Clements,
Structural basis for histone and phosphohistone binding by the GCN5 histone acetyltransferase.
2003,
Pubmed
Doyon,
Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.
2004,
Pubmed
Doyon,
The highly conserved and multifunctional NuA4 HAT complex.
2004,
Pubmed
Durant,
Genome-wide relationships between TAF1 and histone acetyltransferases in Saccharomyces cerevisiae.
2006,
Pubmed
Gordon,
The core histone N-terminal tail domains function independently and additively during salt-dependent oligomerization of nucleosomal arrays.
2005,
Pubmed
,
Xenbase
Hansen,
Assembly and structural properties of subsaturated chromatin arrays.
1993,
Pubmed
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Hudson,
Solution structure and acetyl-lysine binding activity of the GCN5 bromodomain.
2000,
Pubmed
Kaiser,
Enhancement of cyanogen bromide cleavage yields for methionyl-serine and methionyl-threonine peptide bonds.
1999,
Pubmed
Lennox,
Analysis of histone subtypes and their modified forms by polyacrylamide gel electrophoresis.
1989,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
Nucleosome and chromatin fiber dynamics.
2005,
Pubmed
Marmorstein,
Histone acetyltransferases: function, structure, and catalysis.
2001,
Pubmed
Nourani,
Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex.
2001,
Pubmed
Orsi,
Kinetic analysis of progress curves.
1979,
Pubmed
Poux,
Molecular basis for Gcn5/PCAF histone acetyltransferase selectivity for histone and nonhistone substrates.
2003,
Pubmed
Reid,
Coordinate regulation of yeast ribosomal protein genes is associated with targeted recruitment of Esa1 histone acetylase.
2000,
Pubmed
Rojas,
Structure of Tetrahymena GCN5 bound to coenzyme A and a histone H3 peptide.
1999,
Pubmed
Roth,
Histone acetyltransferases.
2001,
Pubmed
Selleck,
The Saccharomyces cerevisiae Piccolo NuA4 histone acetyltransferase complex requires the Enhancer of Polycomb A domain and chromodomain to acetylate nucleosomes.
2005,
Pubmed
Smith,
ESA1 is a histone acetyltransferase that is essential for growth in yeast.
1998,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Takechi,
Sas3 is a histone acetyltransferase and requires a zinc finger motif.
1999,
Pubmed
Tan,
The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.
2005,
Pubmed
Tanner,
Kinetic mechanism of the histone acetyltransferase GCN5 from yeast.
2000,
Pubmed
Tanner,
Catalytic mechanism and function of invariant glutamic acid 173 from the histone acetyltransferase GCN5 transcriptional coactivator.
1999,
Pubmed
Thompson,
Transcriptional coactivator protein p300. Kinetic characterization of its histone acetyltransferase activity.
2001,
Pubmed
,
Xenbase
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Utley,
Regulation of NuA4 histone acetyltransferase activity in transcription and DNA repair by phosphorylation of histone H4.
2005,
Pubmed
Xiao,
Tip60 is a co-repressor for STAT3.
2003,
Pubmed
Yan,
Crystal structure of yeast Esa1 suggests a unified mechanism for catalysis and substrate binding by histone acetyltransferases.
2000,
Pubmed
Yan,
The catalytic mechanism of the ESA1 histone acetyltransferase involves a self-acetylated intermediate.
2002,
Pubmed
de la Cruz,
Do protein motifs read the histone code?
2005,
Pubmed
