Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2007 Feb 21;264:1024-34. doi: 10.1038/sj.emboj.7601562.
Show Gene links
Show Anatomy links
Reconstitution and subunit geometry of human condensin complexes.
Onn I
,
Aono N
,
Hirano M
,
Hirano T
.
???displayArticle.abstract???
Vertebrate cells possess two different condensin complexes, known as condensin I and condensin II, that play a fundamental role in chromosome assembly and segregation during mitosis. Each complex contains a pair of structural maintenance of chromosomes (SMC) ATPases, a kleisin subunit and two HEAT-repeat subunits. Here we use recombinant human condensin subunits to determine their geometry within each complex. We show that both condensin I and condensin II have a pseudo-symmetrical structure, in which the N-terminal half of kleisin links the first HEAT subunit to SMC2, whereas its C-terminal half links the second HEAT subunit to SMC4. No direct interactions are detectable between the SMC dimer and the HEAT subunits, indicating that the kleisin subunit acts as the linchpin in holocomplex assembly. ATP has little, if any, effects on the assembly and integrity of condensin. Cleavage pattern of SMC2 by limited proteolysis is changed upon its binding to ATP or DNA. Our results shed new light on the architecture and dynamics of this highly elaborate machinery designed for chromosome assembly.
Anderson,
Condensin and cohesin display different arm conformations with characteristic hinge angles.
2002, Pubmed,
Xenbase
Anderson,
Condensin and cohesin display different arm conformations with characteristic hinge angles.
2002,
Pubmed
,
Xenbase
Arumugam,
ATP hydrolysis is required for cohesin's association with chromosomes.
2003,
Pubmed
Cobbe,
The evolution of SMC proteins: phylogenetic analysis and structural implications.
2004,
Pubmed
Fennell-Fezzie,
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins.
2005,
Pubmed
Haering,
Molecular architecture of SMC proteins and the yeast cohesin complex.
2002,
Pubmed
Haering,
Structure and stability of cohesin's Smc1-kleisin interaction.
2004,
Pubmed
Hirano,
Positive and negative regulation of SMC-DNA interactions by ATP and accessory proteins.
2004,
Pubmed
Hirano,
At the heart of the chromosome: SMC proteins in action.
2006,
Pubmed
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
Bimodal activation of SMC ATPase by intra- and inter-molecular interactions.
2001,
Pubmed
Hirano,
Hinge-mediated dimerization of SMC protein is essential for its dynamic interaction with DNA.
2002,
Pubmed
Hirano,
ATP-dependent aggregation of single-stranded DNA by a bacterial SMC homodimer.
1998,
Pubmed
Hirano,
Condensins: organizing and segregating the genome.
2005,
Pubmed
Hirano,
Opening closed arms: long-distance activation of SMC ATPase by hinge-DNA interactions.
2006,
Pubmed
Katinka,
Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.
2001,
Pubmed
Kimura,
Dual roles of the 11S regulatory subcomplex in condensin functions.
2000,
Pubmed
,
Xenbase
Kimura,
Chromosome condensation by a human condensin complex in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Kimura,
ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation.
1997,
Pubmed
,
Xenbase
Lammens,
Structural biochemistry of ATP-driven dimerization and DNA-stimulated activation of SMC ATPases.
2004,
Pubmed
Losada,
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
Losada,
Dynamic molecular linkers of the genome: the first decade of SMC proteins.
2005,
Pubmed
Melby,
The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge.
1998,
Pubmed
Nasmyth,
The structure and function of SMC and kleisin complexes.
2005,
Pubmed
Neuwald,
HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions.
2000,
Pubmed
,
Xenbase
Ono,
Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
2003,
Pubmed
,
Xenbase
Saitoh,
ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.
1994,
Pubmed
Saka,
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
1994,
Pubmed
Sakai,
Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly.
2003,
Pubmed
Savvidou,
Drosophila CAP-D2 is required for condensin complex stability and resolution of sister chromatids.
2005,
Pubmed
Schleiffer,
Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners.
2003,
Pubmed
Stray,
Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA.
2003,
Pubmed
,
Xenbase
Strick,
Real-time detection of single-molecule DNA compaction by condensin I.
2004,
Pubmed
,
Xenbase
Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed
Strunnikov,
Condensin and biological role of chromosome condensation.
2003,
Pubmed
Sutani,
DNA renaturation activity of the SMC complex implicated in chromosome condensation.
1997,
Pubmed
Sutani,
Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4.
1999,
Pubmed
,
Xenbase
Weitzer,
A model for ATP hydrolysis-dependent binding of cohesin to DNA.
2003,
Pubmed
Yeong,
Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A.
2003,
Pubmed