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GAGA factor, TFIID, and paused polymerase are present on the hsp70 promoter in Drosophila melanogaster prior to transcriptional activation. In order to investigate the interplay between these components, mutant constructs were analyzed after they had been transformed into flies on P elements. One construct lacked the TATA box and the other lacked the upstream regulatory region where GAGA factor binds. Transcription of each mutant during heat shock was at least 50-fold less than that of a normal promoter construct. Before and after heat shock, both mutant promoters were found to adopt a DNase I hypersensitive state that included the region downstream from the transcription start site. High-resolution analysis of the DNase I cutting pattern identified proteins that could be contributing to the hypersensitivity. GAGA factor footprints were clearly evident in the upstream region of the TATA deletion construct, and a partial footprint possibly caused by TFIID was evident on the TATA box of the upstream deletion construct. Permanganate treatment of intact salivary glands was used to further characterize each promoter construct. Paused polymerase and TFIID were readily detected on the normal promoter construct, whereas both deletions exhibited reduced levels of each of these factors. Hence both the TATA box and the upstream region are required to efficiently recruit TFIID and a paused polymerase to the promoter prior to transcriptional activation. In contrast, GAGA factor appears to be capable of binding and establishing a DNase I hypersensitive region in the absence of TFIID and polymerase. Interestingly, purified GAGA factor was found to bind near the transcription start site, and the strength of this interaction was increased by the presence of the upstream region. GAGA factor alone might be capable of establishing an open chromatin structure that encompasses the upstream regulatory region as well as the core promoter region, thus facilitating the binding of TFIID.
Blau,
Three functional classes of transcriptional activation domain.
1996, Pubmed
Blau,
Three functional classes of transcriptional activation domain.
1996,
Pubmed
Brand,
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
1993,
Pubmed
Chi,
A general mechanism for transcriptional synergy by eukaryotic activators.
1995,
Pubmed
Choy,
Eukaryotic activators function during multiple steps of preinitiation complex assembly.
1993,
Pubmed
Emanuel,
Transcription factor TFIID recognizes DNA sequences downstream of the TATA element in the Hsp70 heat shock gene.
1993,
Pubmed
Giardina,
Promoter melting and TFIID complexes on Drosophila genes in vivo.
1992,
Pubmed
Giardina,
Polymerase processivity and termination on Drosophila heat shock genes.
1993,
Pubmed
Gilmour,
TATA box-dependent protein-DNA interactions are detected on heat shock and histone gene promoters in nuclear extracts derived from Drosophila melanogaster embryos.
1988,
Pubmed
Gilmour,
Drosophila nuclear proteins bind to regions of alternating C and T residues in gene promoters.
1989,
Pubmed
Goodrich,
TBP-TAF complexes: selectivity factors for eukaryotic transcription.
1994,
Pubmed
Hiromi,
Regulation and function of the Drosophila segmentation gene fushi tarazu.
1987,
Pubmed
Hoch,
cis-acting control elements for Krüppel expression in the Drosophila embryo.
1990,
Pubmed
Jackson,
In vivo stage- and tissue-specific DNA-protein interactions at the D. melanogaster alcohol dehydrogenase distal promoter and adult enhancer.
1992,
Pubmed
Kobayashi,
A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.
1995,
Pubmed
Kwon,
Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
1994,
Pubmed
,
Xenbase
Laney,
Redundant control of Ultrabithorax by zeste involves functional levels of zeste protein binding at the Ultrabithorax promoter.
1996,
Pubmed
Lee,
DNA sequence requirements for generating paused polymerase at the start of hsp70.
1992,
Pubmed
Li,
Analyses of promoter-proximal pausing by RNA polymerase II on the hsp70 heat shock gene promoter in a Drosophila nuclear extract.
1996,
Pubmed
,
Xenbase
Lis,
Protein traffic on the heat shock promoter: parking, stalling, and trucking along.
1993,
Pubmed
McGhee,
Reaction of nucleosome DNA with dimethyl sulfate.
1979,
Pubmed
McHugh,
Novel reagents for chemical cleavage at abasic sites and UV photoproducts in DNA.
1995,
Pubmed
O'Brien,
Distribution of GAGA protein on Drosophila genes in vivo.
1995,
Pubmed
Owen-Hughes,
Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex.
1996,
Pubmed
Purnell,
TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes.
1994,
Pubmed
Rasmussen,
Short transcripts of the ternary complex provide insight into RNA polymerase II elongational pausing.
1995,
Pubmed
Rubin,
Genetic transformation of Drosophila with transposable element vectors.
1982,
Pubmed
Shopland,
HSF access to heat shock elements in vivo depends critically on promoter architecture defined by GAGA factor, TFIID, and RNA polymerase II binding sites.
1995,
Pubmed
Simon,
A germline transformation analysis reveals flexibility in the organization of heat shock consensus elements.
1987,
Pubmed
Soeller,
Isolation of cDNAs encoding the Drosophila GAGA transcription factor.
1993,
Pubmed
Tsukiyama,
Purification and properties of an ATP-dependent nucleosome remodeling factor.
1995,
Pubmed
Tsukiyama,
ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
1994,
Pubmed
Verrijzer,
Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II.
1995,
Pubmed
Vincent,
A synthetic homeodomain binding site acts as a cell type specific, promoter specific enhancer in Drosophila embryos.
1990,
Pubmed
Weber,
Genomic footprinting of the hsp70 and histone H3 promoters in Drosophila embryos reveals novel protein-DNA interactions.
1995,
Pubmed
,
Xenbase
Westwood,
Stress-induced oligomerization and chromosomal relocalization of heat-shock factor.
1991,
Pubmed
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
Xiao,
Germline transformation used to define key features of heat-shock response elements.
1988,
Pubmed
Xiao,
Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
1994,
Pubmed
