Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
In amphibian oocytes, the maternal nuclear factor NF7 associates with the elongating pre-mRNAs present on the numerous lateral loops of the lampbrush chromosomes. Here, we have purified NF7 from an oocyte extract by using a combination of ion-exchange chromatography and gel filtration chromatography and demonstrated for the first time that nucleoplasmic NF7 exists primarily as free homotrimers. We confirmed the in vivo homotrimerization of NF7 by using a glutaraldehyde cross-linking assay, and we further showed that it only requires the coiled-coil domain of the NF7 tripartite motif/RBCC motif. Interestingly, we also obtained evidence that NF7 is recruited to the nucleus as a homotrimer, and expression of several mutated forms of NF7 in oocytes demonstrated that both the coiled coil and B box of NF7 are required for its chromosomal association. Together, these data strongly suggest that the interaction of NF7 with the active transcriptional units of RNA polymerase II is mediated by a trimeric B box. Finally, and in agreement with a role for NF7 in pre-mRNA maturation, we obtained evidence supporting the idea that NF7 associates with Cajal bodies.
Barlow,
Structure of the C3HC4 domain by 1H-nuclear magnetic resonance spectroscopy. A new structural class of zinc-finger.
1994, Pubmed
Barlow,
Structure of the C3HC4 domain by 1H-nuclear magnetic resonance spectroscopy. A new structural class of zinc-finger.
1994,
Pubmed
Beenders,
Distribution of XCAP-E and XCAP-D2 in the Xenopus oocyte nucleus.
2003,
Pubmed
,
Xenbase
Bellini,
A putative zinc-binding protein on lampbrush chromosome loops.
1993,
Pubmed
Bellini,
A zinc-binding domain is required for targeting the maternal nuclear protein PwA33 to lampbrush chromosome loops.
1995,
Pubmed
Bellini,
Coilin can form a complex with the U7 small nuclear ribonucleoprotein.
1998,
Pubmed
,
Xenbase
Borden,
The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein PML.
1995,
Pubmed
Borden,
RING domains: master builders of molecular scaffolds?
2000,
Pubmed
Borden,
Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development.
1995,
Pubmed
,
Xenbase
Brzovic,
Structure of a BRCA1-BARD1 heterodimeric RING-RING complex.
2001,
Pubmed
Cao,
Involvement of the rfp tripartite motif in protein-protein interactions and subcellular distribution.
1997,
Pubmed
Cao,
Ret finger protein is a normal component of PML nuclear bodies and interacts directly with PML.
1998,
Pubmed
Casaletto,
Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.
2005,
Pubmed
,
Xenbase
Cubizolles,
pEg7, a new Xenopus protein required for mitotic chromosome condensation in egg extracts.
1998,
Pubmed
,
Xenbase
El-Hodiri,
xnf7 functions in dorsal-ventral patterning of the Xenopus embryo.
1997,
Pubmed
,
Xenbase
Gall,
The centennial of the Cajal body.
2003,
Pubmed
Gall,
Assembly of lampbrush chromosomes from sperm chromatin.
1998,
Pubmed
,
Xenbase
Hanzawa,
The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers.
2001,
Pubmed
Hellman,
Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing.
1995,
Pubmed
Jones,
Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription.
1998,
Pubmed
,
Xenbase
Katoh,
High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides.
2003,
Pubmed
Li,
Xenopus nuclear factor 7 (xnf7) possesses an NLS that functions efficiently in both oocytes and embryos.
1993,
Pubmed
,
Xenbase
Lovering,
Identification and preliminary characterization of a protein motif related to the zinc finger.
1993,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Maresca,
Xnf7 contributes to spindle integrity through its microtubule-bundling activity.
2005,
Pubmed
,
Xenbase
Massiah,
Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING.
2006,
Pubmed
,
Xenbase
Matera,
Pumping RNA: nuclear bodybuilding along the RNP pipeline.
2006,
Pubmed
Meroni,
TRIM/RBCC, a novel class of 'single protein RING finger' E3 ubiquitin ligases.
2005,
Pubmed
Miller,
The nuclear-cytoplasmic distribution of the Xenopus nuclear factor, xnf7, coincides with its state of phosphorylation during early development.
1991,
Pubmed
,
Xenbase
Mische,
Retroviral restriction factor TRIM5alpha is a trimer.
2005,
Pubmed
Morgan,
Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.
2002,
Pubmed
Peculis,
Localization of the nucleolar protein NO38 in amphibian oocytes.
1992,
Pubmed
,
Xenbase
Peng,
Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions.
2000,
Pubmed
Pyne,
Localization of antigens PwA33 and La on lampbrush chromosomes and on nucleoplasmic structures in the oocyte of the urodele Pleurodeles waltl: light and electron microscopic immunocytochemical studies.
1994,
Pubmed
Reddy,
The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Reymond,
The tripartite motif family identifies cell compartments.
2001,
Pubmed
Tafuri,
Dual roles for transcription and translation factors in the RNA storage particles of Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Tsvetkov,
Transcription on lampbrush chromosome loops in the absence of U2 snRNA.
1992,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wolf,
MultiCoil: a program for predicting two- and three-stranded coiled coils.
1997,
Pubmed
Wu,
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
1991,
Pubmed
,
Xenbase
Yap,
Trim5alpha protein restricts both HIV-1 and murine leukemia virus.
2004,
Pubmed
