Kidane AH et al. (2007), Expression and physiological regulation of BDNF...

XB-ART-35996

Gen Comp Endocrinol 2007 Jan 01;1531-3:176-81. doi: 10.1111/j.1440-169X.2007.00927.x.

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Expression and physiological regulation of BDNF receptors in the neuroendocrine melanotrope cell of Xenopus laevis.

Kidane AH , van Dooren SH , Roubos EW , Jenks BG .

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Brain-derived neurotrophic factor (BDNF) and alpha-melanophore-stimulating hormone (alpha-MSH) are co-sequestered in secretory granules in melanotrope cells of the pituitary pars intermedia of the amphibian Xenopus laevis. alpha-MSH is responsible for pigment dispersion in dermal melanophores during the process of black-background adaptation. BDNF-production in melanotrope cells is increased by placing animals on a black background, and BDNF acts as an autocrine stimulatory factor on the melanotrope cells. However, the repertoire of possible neurotrophin receptors of the melanotrope is unknown. In this study we have established the expression of full length TrkB (TrkB.FL), truncated TrkB (TrkB.T) and p75(NTR) receptors in the Xenopus neurointermediate lobe by RT-PCR. In situ hybridization reveals the presence of TrkB.FL mRNA and p75(NTR) mRNA in melanotrope cells. Quantitative RT-PCR shows that in animals on a black background the amounts of TrkB.T and p75(NTR) mRNA are about three times higher than in white background-adapted animals. We suggest that the amount of p75(NTR) sets the sensitivity of the melanotrope cells for the stimulatory action of BDNF during physiological adaptation to background light intensity.

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Species referenced: Xenopus laevis
Genes referenced: bdnf ngfr nradd ntrk2 ntsr1 pomc

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References :

Conlon, Novel neuroendocrine peptides and their receptors and actions. 2007, Pubmed, Xenbase

	Fig. 1. Agarose gel electrophoresis of reaction product of RT-PCR on total mRNA from NILs of black-adapted Xenopus laevis, using primers for TrkB.FL, TrkB.T and p75NTR. Molecular weight markers (M) are indicated on the left.
	Fig. 2. In situ hybridization analysis of TrkB.FL and p75NTR mRNA in the pituitary of black adapted Xenopus. Sections were hybridized with an antisense Dig-labeled p75NTR riboprobe (A and B), or TrkB.FL riboprobe (C and D). B and D are details of A and C, respectively. Arrows indicate nucleus and arrowhead indicates cytoplasm. pd, pars distalis; pi, pars intermedia; pn, pars nervosa; scale bar A and C, 50 μm; B and D, 20 μm.
	Fig. 3. The intracellular domain of TrkB.T of Xenopus and mammals. The first 12 amino acids are identical with full length TrkB (TrkB.FL), and the remaining 11 residues (bold) are specific for truncated TrkB (TrkB.T). The intracellular domain of TrkB.T has a serine residue (italics), which is conserved. Shading indicates homology. xTrkB.T: Xenopus laevis truncated TrkB, Accession No. AAH44959; mTrkB.T: Mus musculus truncated TrkB, Accession No. AAA40482; rTrkB.T: Rattus norvegicus truncated TrkB, Accession No. AAP21832; hTrkB.T: Homo sapiens truncated TrkB, Accession No. AAM77876.