XB-ART-36306
Methods Mol Biol
2007 Jan 01;369:199-212. doi: 10.1007/978-1-59745-294-6_10.
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Cell-free extract systems and the cytoskeleton: preparation of biochemical experiments for transmission electron microscopy.
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Cell-free systems can be used to reconstitute complex actin or microtubule-based phenomena. For example, a number of extracts support actin-dependent propulsion of Listeria monocytogenes, whereas Xenopus laevis extracts support formation of a microtubule-based meiotic spindle. Working in vitro opens these complex processes to biochemical dissection. Here, we describe methods to view these in vitro preparations by thin-section electron microscopy.
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Species referenced: Xenopus laevis
Genes referenced: actl6a