Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Development
2007 Nov 01;13422:4095-106. doi: 10.1242/dev.010272.
Show Gene links
Show Anatomy links
Lrp6 is required for convergent extension during Xenopus gastrulation.
Tahinci E
,
Thorne CA
,
Franklin JL
,
Salic A
,
Christian KM
,
Lee LA
,
Coffey RJ
,
Lee E
.
???displayArticle.abstract???
Wnt signaling regulates beta-catenin-mediated gene transcription and planar cell polarity (PCP). The Wnt co-receptor, Lrp6, is required for signaling along the beta-catenin arm. We show that Lrp6 downregulation (by morpholino injection) or overexpression in Xenopus embryos disrupts convergent extension, a hallmark feature of Wnt/PCP components. In embryos with decreased Lrp6 levels, cells of the dorsal marginal zone (DMZ), which undergoes extensive cellular rearrangements during gastrulation, exhibit decreased length:width ratios, decreased migration, and increased numbers of transient cytoplasmic protrusions. We show that Lrp6 opposes Wnt11 activity and localizes to the posterior edge of migrating DMZ cells and that Lrp6 downregulation enhances cortical and nuclear localization of Dsh and phospho-JNK, respectively. Taken together, these data suggest that Lrp6 inhibits Wnt/PCP signaling. Finally, we identify the region of the Lrp6 protein with Wnt/PCP activity to a stretch of 36 amino acids, distinct from regions required for Wnt/beta-catenin signaling. We propose a model in which Lrp6 plays a critical role in the switch from Wnt/PCP to Wnt/beta-catenin signaling.
Caneparo,
Dickkopf-1 regulates gastrulation movements by coordinated modulation of Wnt/beta catenin and Wnt/PCP activities, through interaction with the Dally-like homolog Knypek.
2007, Pubmed,
Xenbase
Caneparo,
Dickkopf-1 regulates gastrulation movements by coordinated modulation of Wnt/beta catenin and Wnt/PCP activities, through interaction with the Dally-like homolog Knypek.
2007,
Pubmed
,
Xenbase
Ciruna,
Planar cell polarity signalling couples cell division and morphogenesis during neurulation.
2006,
Pubmed
Clevers,
Wnt/beta-catenin signaling in development and disease.
2006,
Pubmed
Cohen,
The hedgehog signaling network.
2003,
Pubmed
Darken,
The planar polarity gene strabismus regulates convergent extension movements in Xenopus.
2002,
Pubmed
,
Xenbase
Davidson,
Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transduction.
2005,
Pubmed
,
Xenbase
Glinka,
Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction.
1998,
Pubmed
,
Xenbase
Goto,
The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus.
2002,
Pubmed
,
Xenbase
Habas,
Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1.
2001,
Pubmed
,
Xenbase
Hyodo-Miura,
XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation.
2006,
Pubmed
,
Xenbase
Itasaki,
Wise, a context-dependent activator and inhibitor of Wnt signalling.
2003,
Pubmed
,
Xenbase
Jenny,
Planar cell polarity signaling: a common mechanism for cellular polarization.
2006,
Pubmed
Jones,
Planar cell polarity signaling in vertebrates.
2007,
Pubmed
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Keller,
Regional expression, pattern and timing of convergence and extension during gastrulation of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Keller,
How we are shaped: the biomechanics of gastrulation.
2003,
Pubmed
,
Xenbase
Kinoshita,
PKC delta is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements.
2003,
Pubmed
,
Xenbase
Klein,
Planar cell polarization: an emerging model points in the right direction.
2005,
Pubmed
Kushner,
A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
1984,
Pubmed
,
Xenbase
Kühl,
Antagonistic regulation of convergent extension movements in Xenopus by Wnt/beta-catenin and Wnt/Ca2+ signaling.
2001,
Pubmed
,
Xenbase
Lane,
Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone.
1997,
Pubmed
,
Xenbase
Li,
Axin and Frat1 interact with dvl and GSK, bridging Dvl to GSK in Wnt-mediated regulation of LEF-1.
1999,
Pubmed
,
Xenbase
Montcouquiol,
Asymmetric localization of Vangl2 and Fz3 indicate novel mechanisms for planar cell polarity in mammals.
2006,
Pubmed
Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase
Penzo-Mendèz,
Activation of Gbetagamma signaling downstream of Wnt-11/Xfz7 regulates Cdc42 activity during Xenopus gastrulation.
2003,
Pubmed
,
Xenbase
Peters,
Casein kinase I transduces Wnt signals.
1999,
Pubmed
,
Xenbase
Sater,
Induction of neuronal differentiation by planar signals in Xenopus embryos.
1993,
Pubmed
,
Xenbase
Sawin,
Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
1992,
Pubmed
,
Xenbase
Schwarz-Romond,
The ankyrin repeat protein Diversin recruits Casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling.
2002,
Pubmed
,
Xenbase
Shih,
Patterns of cell motility in the organizer and dorsal mesoderm of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Shih,
Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Simons,
Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways.
2005,
Pubmed
,
Xenbase
Sokol,
Analysis of Dishevelled signalling pathways during Xenopus development.
1996,
Pubmed
,
Xenbase
Tahinci,
Distinct functions of Rho and Rac are required for convergent extension during Xenopus gastrulation.
2003,
Pubmed
,
Xenbase
Takeuchi,
The prickle-related gene in vertebrates is essential for gastrulation cell movements.
2003,
Pubmed
,
Xenbase
Tamai,
LDL-receptor-related proteins in Wnt signal transduction.
2000,
Pubmed
,
Xenbase
Tamai,
A mechanism for Wnt coreceptor activation.
2004,
Pubmed
,
Xenbase
Tao,
Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos.
2005,
Pubmed
,
Xenbase
Ueno,
Planar cell polarity genes and neural tube closure.
2003,
Pubmed
,
Xenbase
Wallingford,
Xenopus Dishevelled signaling regulates both neural and mesodermal convergent extension: parallel forces elongating the body axis.
2001,
Pubmed
,
Xenbase
Wang,
Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway.
2005,
Pubmed
Wehrli,
arrow encodes an LDL-receptor-related protein essential for Wingless signalling.
2000,
Pubmed
Winklbauer,
Directional mesoderm cell migration in the Xenopus gastrula.
1991,
Pubmed
,
Xenbase
Yamanaka,
JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates.
2002,
Pubmed
,
Xenbase
Yan,
Cell autonomous regulation of multiple Dishevelled-dependent pathways by mammalian Nkd.
2001,
Pubmed
,
Xenbase
Yanagawa,
The dishevelled protein is modified by wingless signaling in Drosophila.
1995,
Pubmed
Yokota,
A novel role for a nodal-related protein; Xnr3 regulates convergent extension movements via the FGF receptor.
2003,
Pubmed
,
Xenbase
Zeng,
A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation.
2005,
Pubmed
,
Xenbase
