Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem Biophys Res Commun
2008 Jan 25;3654:814-20. doi: 10.1016/j.bbrc.2007.11.067.
Show Gene links
Show Anatomy links
Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking.
Liu Z
,
Li H
,
Soleimani M
,
Girijashanker K
,
Reed JM
,
He L
,
Dalton TP
,
Nebert DW
.
???displayArticle.abstract???
The mouse Slc39a8 gene encodes the ZIP8 transporter, which has been shown to be a divalent cation/HCO3- symporter. Using ZIP8 cRNA-injected Xenopus oocyte cultures, we show herein that: [a] ZIP8-mediated cadmium (Cd(2+)) and zinc (Zn(2+)) uptake have V(max) values of 1.8+/-0.08 and 1.0+/-0.08 pmol/oocyte/h, and K(m) values of 0.48+/-0.08 and 0.26+/-0.09 microM, respectively; [b] ZIP8-mediated Cd(2+) uptake is most inhibited by Zn(2+), second-best inhibited by Cu(2+), Pb(2+) and Hg(2+), and not inhibited by Mn(2+) or Fe(2+); and [c] electrogenicity studies demonstrate an influx of two HCO3- anions per one Cd(2+) (or one Zn(2+)) cation, i.e. electroneutral complexes. Using Madin-Darby canine kidney (MDCK) polarized epithelial cells retrovirally infected with ZIP8 cDNA and tagged with hemagglutinin at the C-terminus, we show that-similar to ZIP4-the ZIP8 eight-transmembrane protein is largely internalized during Zn(2+) homeostasis, but moves predominantly to the cell surface membrane (trafficking) under conditions of Zn(2+) depletion.
Bannon,
Effect of DMT1 knockdown on iron, cadmium, and lead uptake in Caco-2 cells.
2003, Pubmed
Bannon,
Effect of DMT1 knockdown on iron, cadmium, and lead uptake in Caco-2 cells.
2003,
Pubmed
Bergeron,
Reciprocal inhibition of Cd(2+) and Ca(2+) uptake in human intestinal crypt cells for voltage-independent Zn-activated pathways.
2006,
Pubmed
Bressler,
Divalent metal transporter 1 in lead and cadmium transport.
2004,
Pubmed
Dalton,
Identification of mouse SLC39A8 as the transporter responsible for cadmium-induced toxicity in the testis.
2005,
Pubmed
Di Paolo,
Phosphoinositides in cell regulation and membrane dynamics.
2006,
Pubmed
Dufner-Beattie,
The adaptive response to dietary zinc in mice involves the differential cellular localization and zinc regulation of the zinc transporters ZIP4 and ZIP5.
2004,
Pubmed
Dufner-Beattie,
The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice.
2003,
Pubmed
Elisma,
Evidence for cadmium uptake through Nramp2: metal speciation studies with Caco-2 cells.
2001,
Pubmed
Erkmann,
Nuclear import of the stem-loop binding protein and localization during the cell cycle.
2005,
Pubmed
Falcone,
Both the 5' untranslated region and the sequences surrounding the start site contribute to efficient initiation of translation in vitro.
1991,
Pubmed
,
Xenbase
Girijashanker,
Slc39a14 gene encodes ZIP14, a metal/bicarbonate symporter: similarities to the ZIP8 transporter.
2008,
Pubmed
He,
ZIP8, member of the solute-carrier-39 (SLC39) metal-transporter family: characterization of transporter properties.
2006,
Pubmed
Hinkle,
Cadmium toxicity in rat pheochromocytoma cells: studies on the mechanism of uptake.
1994,
Pubmed
Järup,
Hazards of heavy metal contamination.
2003,
Pubmed
Järup,
Health effects of cadmium exposure--a review of the literature and a risk estimate.
1998,
Pubmed
Järup,
Cadmium overload and toxicity.
2002,
Pubmed
Kim,
Zn2+-stimulated endocytosis of the mZIP4 zinc transporter regulates its location at the plasma membrane.
2004,
Pubmed
Li,
Missense mutations in Na+:HCO3- cotransporter NBC1 show abnormal trafficking in polarized kidney cells: a basis of proximal renal tubular acidosis.
2005,
Pubmed
,
Xenbase
Liuzzi,
Zip14 (Slc39a14) mediates non-transferrin-bound iron uptake into cells.
2006,
Pubmed
McNiven,
Vesicle formation at the plasma membrane and trans-Golgi network: the same but different.
2006,
Pubmed
Okubo,
Cadmium transport by human Nramp 2 expressed in Xenopus laevis oocytes.
2003,
Pubmed
,
Xenbase
Olivi,
Maitotoxin stimulates Cd influx in Madin-Darby kidney cells by activating Ca-permeable cation channels.
2000,
Pubmed
Olivi,
Involvement of DMT1 in uptake of Cd in MDCK cells: role of protein kinase C.
2001,
Pubmed
Park,
Intestinal absorption of cadmium is associated with divalent metal transporter 1 in rats.
2002,
Pubmed
Shibuya,
Calcium channels in rat melanotrophs are permeable to manganese, cobalt, cadmium, and lanthanum, but not to nickel: evidence provided by fluorescence changes in fura-2-loaded cells.
1992,
Pubmed
Tallkvist,
DMT1 gene expression and cadmium absorption in human absorptive enterocytes.
2001,
Pubmed
,
Xenbase
Waalkes,
Cadmium carcinogenesis.
2003,
Pubmed
Waisberg,
Molecular and cellular mechanisms of cadmium carcinogenesis.
2003,
Pubmed
Wang,
Enhanced cadmium-induced testicular necrosis and renal proximal tubule damage caused by gene-dose increase in a Slc39a8-transgenic mouse line.
2007,
Pubmed
Wang,
Acrodermatitis enteropathica mutations affect transport activity, localization and zinc-responsive trafficking of the mouse ZIP4 zinc transporter.
2004,
Pubmed
Zalups,
Molecular handling of cadmium in transporting epithelia.
2003,
Pubmed