XB-ART-37702
Mol Biol Cell
2008 Jul 01;197:3097-110. doi: 10.1091/mbc.e07-11-1202.
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Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.
Ducat D
,
Kawaguchi S
,
Liu H
,
Yates JR
,
Zheng Y
.
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To identify novel proteins important for microtubule assembly in mitosis, we have used a centrosome-based complementation assay to enrich for proteins with mitotic functions. An RNA interference (RNAi)-based screen of these proteins allowed us to uncover 13 novel mitotic regulators. We carried out in-depth analyses of one of these proteins, Pontin, which is known to have several functions in interphase, including chromatin remodeling, DNA repair, and transcription. We show that reduction of Pontin by RNAi resulted in defects in spindle assembly in Drosophila S2 cells and in several mammalian tissue culture cell lines. Further characterization of Pontin in Xenopus egg extracts demonstrates that Pontin interacts with the gamma tubulin ring complex (gamma-TuRC). Because depletion of Pontin leads to defects in the assembly and organization of microtubule arrays in egg extracts, our studies suggest that Pontin has a mitosis-specific function in regulating microtubule assembly.
???displayArticle.pubmedLink??? 18463163
???displayArticle.pmcLink??? PMC2441676
???displayArticle.link??? Mol Biol Cell
???displayArticle.grants??? [+]
GM-56312 NIGMS NIH HHS , MH-067880 NIMH NIH HHS, P41 RR-011823 NCRR NIH HHS , Howard Hughes Medical Institute , R01 MH067880 NIMH NIH HHS, R01 GM056312 NIGMS NIH HHS , P41 RR011823 NCRR NIH HHS
Species referenced: Xenopus
Genes referenced: ruvbl1
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