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BMC Dev Biol May 28, 2008; 8 58.

Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development.

Strasser MJ , Mackenzie NC , Dumstrei K , Nakkrasae LI , Stebler J , Raz E .

BACKGROUND: Zebrafish germ cells contain granular-like structures, organized around the cell nucleus. These structures share common features with polar granules in Drosophila, germinal granules in Xenopus and chromatoid bodies in mice germ cells, such as the localization of the zebrafish Vasa, Piwi and Nanos proteins, among others. Little is known about the structure of these granules as well as their segregation in mitosis during early germ-cell development. RESULTS: Using transgenic fish expressing a fluorescently labeled novel component of Zebrafish germ cell granules termed Granulito, we followed the morphology and distribution of the granules. We show that whereas these granules initially exhibit a wide size variation, by the end of the first day of development they become a homogeneous population of medium size granules. We investigated this resizing event and demonstrated the role of microtubules and the minus-end microtubule dependent motor protein Dynein in the process. Last, we show that the function of the germ cell granule resident protein the Tudor domain containing protein-7 (Tdrd7) is required for determination of granule morphology and number. CONCLUSION: Our results suggest that Zebrafish germ cell granules undergo a transformation process, which involves germ cell specific proteins as well as the microtubular network.

PubMed ID: 18507824
PMC ID: PMC2441585
Article link: BMC Dev Biol

Species referenced: Xenopus
Genes referenced: arhgef7 clip1 ddx4 fubp1 nos1 nup155 nup58 pgc piwil1 tdrd7

Article Images: [+] show captions
References [+] :
Arkov, The role of Tudor domains in germline development and polar granule architecture. 2006, Pubmed