Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Physiol Genomics
2008 Aug 15;343:265-76. doi: 10.1152/physiolgenomics.90259.2008.
Show Gene links
Show Anatomy links
Cloning and characterization of novel human SLC4A8 gene products encoding Na+-driven Cl-/HCO3(-) exchanger variants NDCBE-A, -C, and -D.
Parker MD
,
Bouyer P
,
Daly CM
,
Boron WF
.
???displayArticle.abstract???
The reported sequences of the human and mouse Na+-driven Cl-/HCO3(-) exchangers (NDCBEs) differ greatly in their extreme cytosolic COOH termini (Ct). In human NDCBE (NDCBE-B), a 17-amino acid (aa) sequence replaces 66 aa at the equivalent position in mouse NDCBE (NDCBE-A). We performed 5'- and 3'-rapid amplification of cDNA ends (RACE) on human brain cDNA, followed by PCR of full-length cDNAs to determine whether the human SLC4A8 gene was capable of producing the mouselike Ct sequence. Our study confirmed the presence in human cDNA of mouse NDCBE-like transcripts (human NDCBE-A) and also disclosed the existence of three further novel NDCBE transcripts that we have called NDCBE-C, NDCBE-D, and NDCBE-D'. The novel NDCBE-C/D/D' transcripts initiate at a novel "exon 0" positioned approximately 35 kb upstream of the first exon of NDCBE-A/B. NDCBE-C/D/D' protein products are predicted to be truncated by 54 aa in the cytosolic NH(2) terminus (Nt) compared with NDCBE-A/B. Our data, combined with a new in silico analysis of partial transcripts reported by others in the region of the human SLC4A8 gene, increase the known extent of the SLC4A8 gene by 49 kb, to 124 kb. A functional comparison of NDCBE-A/B/C/D expressed in Xenopus oocytes demonstrates that the Nt variation does not affect the basal functional expression of NDCBE, but those with the shorter Ct have a 25-50% reduced functional expression compared with those with the longer Ct. By comparison with an artificially truncated NDCBE that contains neither 17-aa nor 66-aa Ct cassette, we determined that the functional difference is unrelated to the 66-aa cassette of NDCBE-A/C, but is instead due to an inhibitory effect of the 17-aa cassette of NDCBE-B/D.
Abuladze,
Structural organization of the human NBC1 gene: kNBC1 is transcribed from an alternative promoter in intron 3.
2000, Pubmed
Abuladze,
Structural organization of the human NBC1 gene: kNBC1 is transcribed from an alternative promoter in intron 3.
2000,
Pubmed
Alpern,
Basolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes.
1987,
Pubmed
Amlal,
Characterization of Na+/HCO-3 cotransporter isoform NBC-3.
1999,
Pubmed
Ando,
IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor.
2003,
Pubmed
Bevensee,
An electrogenic Na(+)-HCO(-)(3) cotransporter (NBC) with a novel COOH-terminus, cloned from rat brain.
2000,
Pubmed
,
Xenbase
Boron,
Intracellular pH transients in squid giant axons caused by CO2, NH3, and metabolic inhibitors.
1976,
Pubmed
Boron,
Active proton transport stimulated by CO2/HCO3-, blocked by cyanide.
1976,
Pubmed
Boron,
Influence of cyclic AMP on intracellular pH regulation and chloride fluxes in barnacle muscle fibers.
1978,
Pubmed
Boron,
pH regulation in barnacle muscle fibers: dependence on intracellular and extracellular pH.
1979,
Pubmed
Boron,
pH regulation in barnacle muscle fibers: dependence on extracellular sodium and bicarbonate.
1981,
Pubmed
Boron,
Stoichiometry and ion dependencies of the intracellular-pH-regulating mechanism in squid giant axons.
1983,
Pubmed
Brosius,
The major kidney band 3 gene transcript predicts an amino-terminal truncated band 3 polypeptide.
1989,
Pubmed
,
Xenbase
Chen,
Expression and localization of Na-driven Cl-HCO(3)(-) exchanger (SLC4A8) in rodent CNS.
2008,
Pubmed
,
Xenbase
Gill,
Preliminary X-ray diffraction analysis of the cytoplasmic N-terminal domain of the Na/HCO3 cotransporter NBCe1-A.
2006,
Pubmed
Grichtchenko,
Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.
2001,
Pubmed
,
Xenbase
Guggino,
Chloride transport across the basolateral cell membrane of the Necturus proximal tubule: dependence on bicarbonate and sodium.
1983,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kozak,
At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.
1987,
Pubmed
L'Allemain,
Role of a Na+-dependent Cl-/HCO3- exchange in regulation of intracellular pH in fibroblasts.
1985,
Pubmed
McAlear,
Electrogenic Na/HCO3 cotransporter (NBCe1) variants expressed in Xenopus oocytes: functional comparison and roles of the amino and carboxy termini.
2006,
Pubmed
,
Xenbase
Moody,
The ionic mechanism of intracellular pH regulation in crayfish neurones.
1981,
Pubmed
Mrowiec,
14-3-3 proteins in membrane protein transport.
2006,
Pubmed
Parker,
Characterization of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl- self-exchange activity.
2008,
Pubmed
,
Xenbase
Piermarini,
Evidence against a direct interaction between intracellular carbonic anhydrase II and pure C-terminal domains of SLC4 bicarbonate transporters.
2007,
Pubmed
Puntervoll,
ELM server: A new resource for investigating short functional sites in modular eukaryotic proteins.
2003,
Pubmed
Romero,
The SLC4 family of HCO 3 - transporters.
2004,
Pubmed
Romero,
Expression cloning and characterization of a renal electrogenic Na+/HCO3- cotransporter.
1997,
Pubmed
,
Xenbase
Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase
Russell,
Role of choloride transport in regulation of intracellular pH.
1976,
Pubmed
Russell,
Intracellular pH and Na fluxes in barnacle muscle with evidence for reversal of the ionic mechanism of intracellular pH regulation.
1983,
Pubmed
Schwiening,
Regulation of intracellular pH in pyramidal neurones from the rat hippocampus by Na(+)-dependent Cl(-)-HCO3- exchange.
1994,
Pubmed
Shirakabe,
IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3- cotransporter 1 (pNBC1).
2006,
Pubmed
,
Xenbase
Siebens,
Effect of electroneutral luminal and basolateral lactate transport on intracellular pH in salamander proximal tubules.
1987,
Pubmed
Thomas,
Ionic mechanism of the H+ pump in a snail neurone.
1976,
Pubmed
Thomas,
The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones.
1977,
Pubmed
Toye,
The human NBCe1-A mutant R881C, associated with proximal renal tubular acidosis, retains function but is mistargeted in polarized renal epithelia.
2006,
Pubmed
,
Xenbase
Trudeau,
HERG, a human inward rectifier in the voltage-gated potassium channel family.
1995,
Pubmed
Vince,
Identification of the carbonic anhydrase II binding site in the Cl(-)/HCO(3)(-) anion exchanger AE1.
2000,
Pubmed
Vince,
Localization of the Cl-/HCO3- anion exchanger binding site to the amino-terminal region of carbonic anhydrase II.
2000,
Pubmed
Wang,
Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution.
2001,
Pubmed
,
Xenbase
Woodgett,
Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements.
1986,
Pubmed
Zhang,
Crystallographic structure and functional interpretation of the cytoplasmic domain of erythrocyte membrane band 3.
2000,
Pubmed
Zhou,
Monomeric 14-3-3 protein is sufficient to modulate the activity of the Drosophila slowpoke calcium-dependent potassium channel.
2003,
Pubmed
