Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2008 Jun 01;2812:3905-16. doi: 10.1128/MCB.02116-07.
Show Gene links
Show Anatomy links
The anaphase-promoting complex/cyclosome controls repair and recombination by ubiquitylating Rhp54 in fission yeast.
Trickey M
,
Grimaldi M
,
Yamano H
.
???displayArticle.abstract???
Homologous recombination (HR) is important for maintaining genome integrity and for the process of meiotic chromosome segregation and the generation of variation. HR is regulated throughout the cell cycle, being prevalent in the S and G2 phases and suppressed in the G1 phase. Here we show that the anaphase-promoting complex/cyclosome (APC/C) regulates homologous recombination in the fission yeast Schizosaccharomyces pombe by ubiquitylating Rhp54 (an ortholog of Rad54). We show that Rhp54 is a novel APC/C substrate that is destroyed in G1 phase in a KEN-box- and Ste9/Fizzy-related manner. The biological consequences of failing to temporally regulate HR via Rhp54 degradation are seen in haploid cells only in the absence of antirecombinase Srs2 function and are more extensive in diploid cells, which become sensitive to a range of DNA-damaging agents, including hydroxyurea, methyl methanesulfonate, bleomycin, and UV. During meiosis, expression of nondegradable Rhp54 inhibits interhomolog recombination and stimulates sister chromatid recombination. We thus propose that it is critical to control levels of Rhp54 in G1 to suppress HR repair of double-strand breaks and during meiosis to coordinate interhomolog recombination.
Aylon,
The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.
2004, Pubmed
Aylon,
The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.
2004,
Pubmed
Benito,
Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor.
1998,
Pubmed
Busygina,
Hed1 regulates Rad51-mediated recombination via a novel mechanism.
2008,
Pubmed
Caspari,
Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III.
2002,
Pubmed
Catlett,
Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks.
2003,
Pubmed
Chikashige,
Telomere-led premeiotic chromosome movement in fission yeast.
1994,
Pubmed
Chikashige,
Meiotic nuclear reorganization: switching the position of centromeres and telomeres in the fission yeast Schizosaccharomyces pombe.
1997,
Pubmed
Ding,
Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast.
2004,
Pubmed
Doe,
The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.
2004,
Pubmed
Ferreira,
Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle.
2004,
Pubmed
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Harper,
The anaphase-promoting complex: it's not just for mitosis any more.
2002,
Pubmed
Henderson,
Cyclin-dependent kinase directly regulates initiation of meiotic recombination.
2006,
Pubmed
Hershko,
The ubiquitin system.
1998,
Pubmed
Heyer,
Rad54: the Swiss Army knife of homologous recombination?
2006,
Pubmed
Ira,
DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1.
2004,
Pubmed
Khanna,
DNA double-strand breaks: signaling, repair and the cancer connection.
2001,
Pubmed
Kitamura,
Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase.
1998,
Pubmed
Klein,
RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis.
1997,
Pubmed
Krejci,
DNA helicase Srs2 disrupts the Rad51 presynaptic filament.
2003,
Pubmed
Krogh,
Recombination proteins in yeast.
2004,
Pubmed
Limbo,
Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.
2007,
Pubmed
Lorca,
Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Manolis,
Novel functional requirements for non-homologous DNA end joining in Schizosaccharomyces pombe.
2001,
Pubmed
Martin,
RAD51 up-regulation bypasses BRCA1 function and is a common feature of BRCA1-deficient breast tumors.
2007,
Pubmed
Mazin,
A novel function of Rad54 protein. Stabilization of the Rad51 nucleoprotein filament.
2003,
Pubmed
Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed
Muris,
Isolation of the Schizosaccharomyces pombe RAD54 homologue, rhp54+, a gene involved in the repair of radiation damage and replication fidelity.
1996,
Pubmed
Muris,
Homologous recombination in the fission yeast Schizosaccharomyces pombe: different requirements for the rhp51+, rhp54+ and rad22+ genes.
1997,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Nakayama,
Ubiquitin ligases: cell-cycle control and cancer.
2006,
Pubmed
Neale,
Clarifying the mechanics of DNA strand exchange in meiotic recombination.
2006,
Pubmed
Osman,
UV irradiation causes the loss of viable mitotic recombinants in Schizosaccharomyces pombe cells lacking the G(2)/M DNA damage checkpoint.
2002,
Pubmed
Peters,
The anaphase-promoting complex: proteolysis in mitosis and beyond.
2002,
Pubmed
Peters,
The anaphase promoting complex/cyclosome: a machine designed to destroy.
2006,
Pubmed
Petronczki,
Un ménage à quatre: the molecular biology of chromosome segregation in meiosis.
2003,
Pubmed
Petukhova,
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins.
1998,
Pubmed
Pfleger,
The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1.
2000,
Pubmed
,
Xenbase
Pâques,
Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.
1999,
Pubmed
Roeder,
Meiotic chromosomes: it takes two to tango.
1997,
Pubmed
Shinohara,
Tid1/Rdh54 promotes colocalization of rad51 and dmc1 during meiotic recombination.
2000,
Pubmed
Solinger,
Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament.
2001,
Pubmed
Sonoda,
Differential usage of non-homologous end-joining and homologous recombination in double strand break repair.
2006,
Pubmed
Sugawara,
In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.
2003,
Pubmed
Thomä,
Structure of the SWI2/SNF2 chromatin-remodeling domain of eukaryotic Rad54.
2005,
Pubmed
Thornton,
Precise destruction: an emerging picture of the APC.
2006,
Pubmed
Tsubouchi,
Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired.
2006,
Pubmed
Veaute,
The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments.
2003,
Pubmed
Wells,
Homologous chromosome pairing in Schizosaccharomyces pombe.
2006,
Pubmed
Wiese,
Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement.
2007,
Pubmed
Wolner,
Recruitment of the recombinational repair machinery to a DNA double-strand break in yeast.
2003,
Pubmed
Yamano,
Cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Yamano,
The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.
1998,
Pubmed
Zhang,
Homology-driven chromatin remodeling by human RAD54.
2007,
Pubmed
van Gent,
Chromosomal stability and the DNA double-stranded break connection.
2001,
Pubmed