Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
DNA Repair (Amst)
2008 Dec 01;712:1973-81. doi: 10.1016/j.dnarep.2008.08.005.
Show Gene links
Show Anatomy links
Fanconi anemia proteins stabilize replication forks.
Wang LC
,
Stone S
,
Hoatlin ME
,
Gautier J
.
???displayArticle.abstract???
Fanconi anemia (FA) is a recessive genetic disorder characterized by hypersensitivity to crosslinking agents that has been attributed to defects in DNA repair and/or replication. FANCD2 and the FA core complex bind to chromatin during DNA replication; however, the role of FA proteins during replication is unknown. Using Xenopus cell-free extracts, we show that FANCL depletion results in defective DNA replication restart following treatment with camptothecin, a drug that results in DSBs during DNA replication. This defect is more pronounced following treatment with mitomycin C, presumably because of an additional role of the FA pathway in DNA crosslink repair. Moreover, we show that chromatin binding of FA core complex proteins during DNA replication follows origin assembly and origin firing and is dependent on the binding of RPA to ssDNA while FANCD2 additionally requires ATR, consistent with FA proteins acting at replication forks. Together, our data suggest that FA proteins play a role in replication restart at collapsed replication forks.
Abraham,
Cell cycle checkpoint signaling through the ATM and ATR kinases.
2001, Pubmed
Abraham,
Cell cycle checkpoint signaling through the ATM and ATR kinases.
2001,
Pubmed
Admire,
Cycles of chromosome instability are associated with a fragile site and are increased by defects in DNA replication and checkpoint controls in yeast.
2006,
Pubmed
Akkari,
The 4N cell cycle delay in Fanconi anemia reflects growth arrest in late S phase.
2001,
Pubmed
Alpi,
UBE2T, the Fanconi anemia core complex, and FANCD2 are recruited independently to chromatin: a basis for the regulation of FANCD2 monoubiquitination.
2007,
Pubmed
Auerbach,
A test for Fanconi's anemia.
1988,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Cantor,
BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function.
2001,
Pubmed
Cha,
ATR homolog Mec1 promotes fork progression, thus averting breaks in replication slow zones.
2002,
Pubmed
Chirnomas,
Chemosensitization to cisplatin by inhibitors of the Fanconi anemia/BRCA pathway.
2006,
Pubmed
Ciccia,
Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM.
2007,
Pubmed
Costanzo,
Mre11 protein complex prevents double-strand break accumulation during chromosomal DNA replication.
2001,
Pubmed
,
Xenbase
Dorsman,
Identification of the Fanconi anemia complementation group I gene, FANCI.
2007,
Pubmed
Folias,
BRCA1 interacts directly with the Fanconi anemia protein FANCA.
2002,
Pubmed
Garcia-Higuera,
Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway.
2001,
Pubmed
German,
A test for Fanconi's anemia.
1987,
Pubmed
Gupta,
FANCJ (BACH1) helicase forms DNA damage inducible foci with replication protein A and interacts physically and functionally with the single-stranded DNA-binding protein.
2007,
Pubmed
Ho,
Phosphorylation of FANCD2 on two novel sites is required for mitomycin C resistance.
2006,
Pubmed
Howlett,
Biallelic inactivation of BRCA2 in Fanconi anemia.
2002,
Pubmed
Hussain,
Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways.
2004,
Pubmed
Kennedy,
The Fanconi Anemia/BRCA pathway: new faces in the crowd.
2005,
Pubmed
Labib,
Replication fork barriers: pausing for a break or stalling for time?
2007,
Pubmed
Levitus,
Heterogeneity in Fanconi anemia: evidence for 2 new genetic subtypes.
2004,
Pubmed
Ling,
FAAP100 is essential for activation of the Fanconi anemia-associated DNA damage response pathway.
2007,
Pubmed
Litman,
BACH1 is critical for homologous recombination and appears to be the Fanconi anemia gene product FANCJ.
2005,
Pubmed
Liu,
Mechanism of action of camptothecin.
2000,
Pubmed
Lopes,
The DNA replication checkpoint response stabilizes stalled replication forks.
2001,
Pubmed
Macé,
3R coordination by Fanconi anemia proteins.
2005,
Pubmed
Matsuoka,
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
2007,
Pubmed
Meetei,
A novel ubiquitin ligase is deficient in Fanconi anemia.
2003,
Pubmed
Meetei,
X-linked inheritance of Fanconi anemia complementation group B.
2004,
Pubmed
Meetei,
A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M.
2005,
Pubmed
Mi,
The Fanconi anemia core complex associates with chromatin during S phase.
2005,
Pubmed
Montes de Oca,
Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin.
2005,
Pubmed
Nomura,
Human Mus81 and FANCB independently contribute to repair of DNA damage during replication.
2007,
Pubmed
Pichierri,
Fanconi anemia proteins and the s phase checkpoint.
2004,
Pubmed
Pichierri,
BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks.
2004,
Pubmed
Poll,
Differential sensitivity of Fanconi anaemia lymphocytes to the clastogenic action of cis-diamminedichloroplatinum (II) and trans-diamminedichloroplatinum (II).
1985,
Pubmed
Qiao,
Phosphorylation of fanconi anemia (FA) complementation group G protein, FANCG, at serine 7 is important for function of the FA pathway.
2004,
Pubmed
Reid,
Biallelic mutations in PALB2 cause Fanconi anemia subtype FA-N and predispose to childhood cancer.
2007,
Pubmed
Shechter,
ATR and ATM regulate the timing of DNA replication origin firing.
2004,
Pubmed
,
Xenbase
Sims,
FANCI is a second monoubiquitinated member of the Fanconi anemia pathway.
2007,
Pubmed
Smogorzewska,
Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair.
2007,
Pubmed
Sobeck,
DNA structure-induced recruitment and activation of the Fanconi anemia pathway protein FANCD2.
2007,
Pubmed
,
Xenbase
Sobeck,
Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks.
2006,
Pubmed
,
Xenbase
Stokes,
DNA damage-induced replication arrest in Xenopus egg extracts.
2003,
Pubmed
,
Xenbase
Stone,
Identification, developmental expression and regulation of the Xenopus ortholog of human FANCG/XRCC9.
2007,
Pubmed
,
Xenbase
Tercero,
Regulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint.
2001,
Pubmed
Thompson,
How Fanconi anemia proteins promote the four Rs: replication, recombination, repair, and recovery.
2005,
Pubmed
Trenz,
ATM and ATR promote Mre11 dependent restart of collapsed replication forks and prevent accumulation of DNA breaks.
2006,
Pubmed
,
Xenbase
Wang,
Functional interaction of monoubiquitinated FANCD2 and BRCA2/FANCD1 in chromatin.
2004,
Pubmed
Wang,
Chk1-mediated phosphorylation of FANCE is required for the Fanconi anemia/BRCA pathway.
2007,
Pubmed
Wang,
Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins.
2007,
Pubmed
Xia,
Fanconi anemia is associated with a defect in the BRCA2 partner PALB2.
2007,
Pubmed
Yamashita,
The fanconi anemia pathway requires FAA phosphorylation and FAA/FAC nuclear accumulation.
1998,
Pubmed
Ying,
The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding.
2005,
Pubmed
,
Xenbase
Zou,
Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.
2003,
Pubmed
