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Exp Mol Med
2008 Oct 31;405:505-13. doi: 10.3858/emm.2008.40.5.505.
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Co-localization and interaction of human organic anion transporter 4 with caveolin-1 in primary cultured human placental trophoblasts.
Lee WK
,
Choi JK
,
Cha SH
.
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The human organic anion transporter 4 (hOAT4) has been identified as the fourth isoform of OAT family. hOAT4 contributes to move several negatively charged organic compounds between cells and their extracellular milieu. The functional characteristics and regulatory mechanisms of hOAT4 remain to be elucidated. It is well known that caveolin plays a role in modulating proteins having some biological functions. To address this issue, we investigated the co-localization and interaction between hOAT4 and caveolin-1. hOAT4 and caveolin-1 (mRNA and protein expression) were observed in cultured human placental trophoblasts isolated from placenta. The confocal microscopy of immuno-cytochemistry using primary cultured human trophoblasts showed hOAT4 and caveolin-1 were co-localized at the plasma membrane of the cell. This finding was confirmed by Western blot analysis using isolated caveolae-enriched membrane fractions and immune-precipitates from the trophoblasts. When synthesized cRNA of hOAT4 along with scrambled- or antisense-oligodeoxynucleotide (ODN) of Xenopus caveolin-1 were co-injected to Xenopus oocytes, the [3H]estrone sulfate uptake was significantly decreased by the co-injection of antisense ODN but not by scrambled ODN. These findings suggest that hOAT4 and caveolin-1 share a cellular expression in the plasma membrane and caveolin-1 up-regulates the organic anionic compound uptake by hOAT4 under the normal physiological condition.
Bischof,
Regulation of trophoblastic gelatinases by proto-oncogenes.
2003, Pubmed
Bischof,
Regulation of trophoblastic gelatinases by proto-oncogenes.
2003,
Pubmed
Cai,
Overexpression of caveolin-1 increases plasma membrane fluidity and reduces P-glycoprotein function in Hs578T/Dox.
2004,
Pubmed
Cha,
Evidence for Na+/Ca2+ exchanger 1 association with caveolin-1 and -2 in C6 glioma cells.
2004,
Pubmed
Cha,
Molecular cloning and characterization of multispecific organic anion transporter 4 expressed in the placenta.
2000,
Pubmed
,
Xenbase
Cha,
Identification and characterization of human organic anion transporter 3 expressing predominantly in the kidney.
2001,
Pubmed
,
Xenbase
Couet,
Identification of peptide and protein ligands for the caveolin-scaffolding domain. Implications for the interaction of caveolin with caveolae-associated proteins.
1997,
Pubmed
Ekaratanawong,
Human organic anion transporter 4 is a renal apical organic anion/dicarboxylate exchanger in the proximal tubules.
2004,
Pubmed
Ganapathy,
Placental transporters relevant to drug distribution across the maternal-fetal interface.
2000,
Pubmed
Khamdang,
Interaction of human and rat organic anion transporter 2 with various cephalosporin antibiotics.
2003,
Pubmed
Knipp,
Nutrient transport across the placenta.
1999,
Pubmed
Kusuhara,
Molecular cloning and characterization of a new multispecific organic anion transporter from rat brain.
1999,
Pubmed
,
Xenbase
Kwak,
Evidence for cyclooxygenase-2 association with caveolin-3 in primary cultured rat chondrocytes.
2006,
Pubmed
Kwak,
Evidence for rat organic anion transporter 3 association with caveolin-1 in rat kidney.
2005,
Pubmed
,
Xenbase
Kwak,
Co-localization and interaction of organic anion transporter 1 with caveolin-2 in rat kidney.
2005,
Pubmed
,
Xenbase
Lisanti,
Caveolae, caveolin and caveolin-rich membrane domains: a signalling hypothesis.
1994,
Pubmed
McDonald,
A caveolar complex between the cationic amino acid transporter 1 and endothelial nitric-oxide synthase may explain the "arginine paradox".
1997,
Pubmed
Meier,
Molecular mechanisms of hepatic bile salt transport from sinusoidal blood into bile.
1995,
Pubmed
Miyazaki,
Modulation of renal apical organic anion transporter 4 function by two PDZ domain-containing proteins.
2005,
Pubmed
Monte,
Identification of a novel murine organic anion transporter family member, OAT6, expressed in olfactory mucosa.
2004,
Pubmed
Müller,
Molecular aspects of hepatobiliary transport.
1997,
Pubmed
Pascolo,
Effects of maturation on RNA transcription and protein expression of four MRP genes in human placenta and in BeWo cells.
2003,
Pubmed
Pritchard,
Mechanisms mediating renal secretion of organic anions and cations.
1993,
Pubmed
Razani,
Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis.
2002,
Pubmed
,
Xenbase
Sadler,
Low-density caveolae-like membrane from Xenopus laevis oocytes is enriched in Ras.
,
Pubmed
,
Xenbase
Sekine,
The multispecific organic anion transporter (OAT) family.
2000,
Pubmed
Sekine,
Expression cloning and characterization of a novel multispecific organic anion transporter.
1997,
Pubmed
,
Xenbase
Stulc,
Placental transfer of inorganic ions and water.
1997,
Pubmed
Sun,
Isolation of a family of organic anion transporters from human liver and kidney.
2001,
Pubmed
,
Xenbase
Sweet,
Expression cloning and characterization of ROAT1. The basolateral organic anion transporter in rat kidney.
1997,
Pubmed
,
Xenbase
Ugele,
Characterization and identification of steroid sulfate transporters of human placenta.
2003,
Pubmed
Ullrich,
Renal transport mechanisms for xenobiotics: chemicals and drugs.
1993,
Pubmed
Youngblood,
Identification and functional assessment of the novel murine organic anion transporter Oat5 (Slc22a19) expressed in kidney.
2004,
Pubmed
,
Xenbase
Zhou,
Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
2008,
Pubmed
Zhou,
Regulation of human organic anion transporter 4 by progesterone and protein kinase C in human placental BeWo cells.
2007,
Pubmed
