Int J Dev Biol
January 1, 2008;
Cloning and developmental expression of the soxB2 genes, sox14 and sox21, during Xenopus laevis embryogenesis.
The Sox family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Here we describe the cloning of the X. laevis orthologs of the SoxB2 family of transcription factors, sox14
. In situ hybridization revealed that sox14
expression is restricted to the hypothalamus
, dorsal thalamus
, the optic tectum
, a region of the somatic motornucleus in the midbrain
, the vestibular nuclei in the hindbrain
and a discrete ventral
domain in the developing spinal cord
. In contrast to the limited expression domain of sox14
is found throughout the developing central nervous system
, including the olfactory placodes, with strongest expression at the boundary between the midbrain
Int J Dev Biol
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Fig. 3. Spatio-temporal expression pattern of sox14. (A) RT-PCR analysis from oocytes and embryos to stage 38 as indicated across the top. ODC (lower panel) was used as the loading control. (B-I) In situ hybridization of sox14 at indicated stages. In all figures, anterior is to the left. (B) Lateral view; (C) dorsal view, anterior view in the inset. (D) Sagittal sections of stage 38, double in situ of sox14 and en2 in the inset. (E) Dissected brain from stage 38 embryo. (F-I) Transverse sections from stage 32 (I) and 38 (F-H) embryos. Symbols: bracket, expression in vestibular nuclei; black arrowhead, dorsal thalamus; open arrowhead, dorsal hindbrain sox14 positive cells; M, midbrain; hy, hypothalamus; and AM, abducens motornucleus; mhb, midbrain hindbrain barrier; OT, optic tectum; DT, dorsal thalamus; OM, oculomotor motornucleus; TM, trochlear motornucleus; IN, interneurons. Black bar equivalent to 100 μm.
Fig. 4. Spatio-temporal expression pattern of sox21. (A) RT-PCR analysis of embryos from oocyte to stage 36 as indicated across the top. ODC was used as the loading control. (B,C,F,J,N,P) In situ hybridization of embryos stained for sox21 at indicated stages and (F-M, O) for regional brain markers as indicated. (B,C, J-M, inset P) Anterior views. (N-P) Dorsal views. (F-I) Lateral views. (D,E) Transverse sections generated from a stage 31 embryo. Symbols: Black asterisks, midbrain-hindbrain boundary; f, forebrain; h, hindbrain; m, midbrain; hy, hypothalamus; n, notochord; op, olfactory placode; ov, otic vesicle.
The LIM-homeodomain gene family in the developing Xenopus brain: conservation and divergences with the mouse related to the evolution of the forebrain.