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Nucleic Acids Res April 1, 2009; 37 (6): 1907-14.
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U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing.

Fukumura K , Taniguchi I , Sakamoto H , Ohno M , Inoue K .

U1 snRNP plays a crucial role in the 5'' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, hF1gamma intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1gamma exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

PubMed ID: 19190090
PMC ID: PMC2665233
Article link: Nucleic Acids Res

Species referenced: Xenopus
Genes referenced: myc rbfox1 u2af2

Article Images: [+] show captions
References [+] :
Ben-Dov, Genome-wide analysis of alternative pre-mRNA splicing. 2008, Pubmed