Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Renal Physiol
2009 Jul 01;2971:F36-45. doi: 10.1152/ajprenal.90559.2008.
Show Gene links
Show Anatomy links
MUPP1 complexes renal K+ channels to alter cell surface expression and whole cell currents.
Sindic A
,
Huang C
,
Chen AP
,
Ding Y
,
Miller-Little WA
,
Che D
,
Romero MF
,
Miller RT
.
???displayArticle.abstract???
We previously found that the Ca(2+)-sensing receptor (CaR) interacts with and inactivates the inwardly rectifying K(+) channel Kir4.2 that is expressed in the kidney cortex and that has a COOH-terminal PDZ domain. To identify potential scaffolding proteins that could organize a macromolecular signaling complex involving the CaR and Kir4.2, we used yeast two-hybrid cloning with the COOH-terminal 125 amino acids (AA) of Kir4.2 as bait to screen a human kidney cDNA library. We identified two independent partial cDNAs corresponding to the COOH-terminal 900 AA of MUPP1, a protein containing 13 PDZ binding domains that is expressed in the kidney in tight junctions and lateral borders of epithelial cells. When expressed in human embryonic kidney (HEK)-293 cells, Kir4.2 coimmunoprecipitates reciprocally with MUPP1 but not with a Kir4.2 construct lacking the four COOH-terminal amino acids, Kir5.1, or the CaR. MUPP1 and Kir4.2 coimmunoprecipitate reciprocally from rat kidney cortex extracts. Coexpression of MUPP1 with Kir4.2 in HEK-293 cells leads to reduced cell surface expression of Kir4.2 as assessed by cell surface biotinylation. Coexpression of MUPP1 and Kir4.2 in Xenopus oocytes results in reduced whole cell currents compared with expression of Kir4.2 alone, whereas expression of Kir4.2DeltaPDZ results in minimal currents and is not affected by coexpression with MUPP1. Immunofluorescence studies of oocytes demonstrate that MUPP1 reduces Kir4.2 membrane localization. These results indicate that Kir4.2 interacts selectively with MUPP1 to affect its cell surface expression. Thus MUPP1 and Kir4.2 may participate in a protein complex in the nephron that could regulate transport of K(+) as well as other ions.
Adachi,
Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins.
2009, Pubmed
Adachi,
Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins.
2009,
Pubmed
Alewine,
TIP-1 has PDZ scaffold antagonist activity.
2006,
Pubmed
Awata,
Interaction of the calcium-sensing receptor and filamin, a potential scaffolding protein.
2001,
Pubmed
Balasubramanian,
GABAB receptor association with the PDZ scaffold Mupp1 alters receptor stability and function.
2007,
Pubmed
Becamel,
Interaction of serotonin 5-hydroxytryptamine type 2C receptors with PDZ10 of the multi-PDZ domain protein MUPP1.
2001,
Pubmed
Brown,
Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.
1993,
Pubmed
,
Xenbase
Brône,
PDZ proteins retain and regulate membrane transporters in polarized epithelial cell membranes.
2005,
Pubmed
Casamassima,
Identification of a heteromeric interaction that influences the rectification, gating, and pH sensitivity of Kir4.1/Kir5.1 potassium channels.
2003,
Pubmed
,
Xenbase
Chung,
A new crucial protein interaction element that targets the adenovirus E4-ORF1 oncoprotein to membrane vesicles.
2007,
Pubmed
Coyne,
The coxsackievirus and adenovirus receptor interacts with the multi-PDZ domain protein-1 (MUPP-1) within the tight junction.
2004,
Pubmed
Flagg,
Molecular mechanism of a COOH-terminal gating determinant in the ROMK channel revealed by a Bartter's disease mutation.
2002,
Pubmed
,
Xenbase
Gottardi,
Biotinylation and assessment of membrane polarity: caveats and methodological concerns.
1995,
Pubmed
Hamazaki,
Multi-PDZ domain protein 1 (MUPP1) is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule.
2002,
Pubmed
Hill,
Cloning, expression, and localization of a rat hepatocyte inwardly rectifying potassium channel.
2002,
Pubmed
Horio,
Clustering and enhanced activity of an inwardly rectifying potassium channel, Kir4.1, by an anchoring protein, PSD-95/SAP90.
1997,
Pubmed
Huang,
Parallel activation of phosphatidylinositol 4-kinase and phospholipase C by the extracellular calcium-sensing receptor.
2002,
Pubmed
Huang,
Direct activation of inward rectifier potassium channels by PIP2 and its stabilization by Gbetagamma.
1998,
Pubmed
,
Xenbase
Huang,
Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function.
2007,
Pubmed
,
Xenbase
Ide,
Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells.
1999,
Pubmed
Ivanova,
Cellular localization of MAGI-1 caspase cleavage products and their role in apoptosis.
2007,
Pubmed
Kimber,
Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo.
2002,
Pubmed
Konstas,
Identification of domains that control the heteromeric assembly of Kir5.1/Kir4.0 potassium channels.
2003,
Pubmed
,
Xenbase
Krapivinsky,
SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation.
2004,
Pubmed
Kurschner,
CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins.
1998,
Pubmed
Lachheb,
Kir4.1/Kir5.1 channel forms the major K+ channel in the basolateral membrane of mouse renal collecting duct principal cells.
2008,
Pubmed
Lam,
Modulation of Kir4.2 rectification properties and pHi-sensitive run-down by association with Kir5.1.
2006,
Pubmed
Lanaspa,
The tight junction protein, MUPP1, is up-regulated by hypertonicity and is important in the osmotic stress response in kidney cells.
2007,
Pubmed
Lanaspa,
Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells.
2008,
Pubmed
Latorre,
Viral oncoprotein-induced mislocalization of select PDZ proteins disrupts tight junctions and causes polarity defects in epithelial cells.
2005,
Pubmed
Leyland,
An alternatively spliced isoform of PSD-93/chapsyn 110 binds to the inwardly rectifying potassium channel, Kir2.1.
2004,
Pubmed
Lourdel,
An inward rectifier K(+) channel at the basolateral membrane of the mouse distal convoluted tubule: similarities with Kir4-Kir5.1 heteromeric channels.
2002,
Pubmed
Mancini,
The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity.
2000,
Pubmed
Milewski,
PDZ-binding motifs are unable to ensure correct polarized protein distribution in the absence of additional localization signals.
2005,
Pubmed
Olsen,
Basolateral membrane expression of the Kir 2.3 channel is coordinated by PDZ interaction with Lin-7/CASK complex.
2002,
Pubmed
,
Xenbase
Olsen,
Differential localization of mammalian Lin-7 (MALS/Veli) PDZ proteins in the kidney.
2005,
Pubmed
Ookata,
Localization of inward rectifier potassium channel Kir7.1 in the basolateral membrane of distal nephron and collecting duct.
2000,
Pubmed
Pearson,
Expression of a functional Kir4 family inward rectifier K+ channel from a gene cloned from mouse liver.
1999,
Pubmed
,
Xenbase
Pessia,
Differential pH sensitivity of Kir4.1 and Kir4.2 potassium channels and their modulation by heteropolymerisation with Kir5.1.
2001,
Pubmed
,
Xenbase
Pessia,
Subunit positional effects revealed by novel heteromeric inwardly rectifying K+ channels.
1996,
Pubmed
,
Xenbase
Poliak,
Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells.
2002,
Pubmed
Sciortino,
Cation and voltage dependence of rat kidney electrogenic Na(+)-HCO(-)(3) cotransporter, rkNBC, expressed in oocytes.
1999,
Pubmed
,
Xenbase
Sheng,
Ion channel targeting in neurons.
1997,
Pubmed
Shenolikar,
Regulation of ion transport by the NHERF family of PDZ proteins.
2004,
Pubmed
Sitek,
Expression of MUPP1 protein in mouse brain.
2003,
Pubmed
Staub,
Regulation of ion transport by protein-protein interaction domains.
1997,
Pubmed
Tanemoto,
PSD-95 mediates formation of a functional homomeric Kir5.1 channel in the brain.
2002,
Pubmed
Tanemoto,
PDZ binding motif-dependent localization of K+ channel on the basolateral side in distal tubules.
2004,
Pubmed
Tanemoto,
PDZ-binding and di-hydrophobic motifs regulate distribution of Kir4.1 channels in renal cells.
2005,
Pubmed
Tanemoto,
MAGI-1a functions as a scaffolding protein for the distal renal tubular basolateral K+ channels.
2008,
Pubmed
Thiery,
Developmentally regulated expression of the murine ortholog of the potassium channel KIR4.2 (KCNJ15).
2000,
Pubmed
Ullmer,
Cloning and characterization of MUPP1, a novel PDZ domain protein.
1998,
Pubmed
Vogelmann,
Breaking into the epithelial apical-junctional complex--news from pathogen hackers.
2004,
Pubmed
Wegmann,
Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts.
2004,
Pubmed
Wu,
An inwardly rectifying potassium channel in apical membrane of Calu-3 cells.
2004,
Pubmed
Yoo,
Assembly and trafficking of a multiprotein ROMK (Kir 1.1) channel complex by PDZ interactions.
2004,
Pubmed
,
Xenbase
van de Pavert,
Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure.
2004,
Pubmed
