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XB-ART-40026
Int J Dev Biol January 1, 2010; 54 (4): 609-15.

XRASGRP2 is essential for blood vessel formation during Xenopus development.

Suzuki K , Takahashi S , Haramoto Y , Onuma Y , Nagamine K , Okabayashi K , Hashizume K , Iwanaka T , Asashima M .


Abstract
Ras guanyl nucleotide-releasing protein 2 (RASGRP2), one of the Ras guanine exchange factors, is implicated as a critical regulator of inside-out integrin activation in human lymphocytes, neutrophils and platelets. However, the activities of this protein in endothelial cells remain unclear. In the current study, we identify a physiological function in blood vessel formation for XRASGRP2, which is the Xenopus ortholog of mammalian RASGRP2. XRASGRP2 over-expression induced ectopic vascular formation, and XRASGRP2-knockdown embryos showed delayed vascular development. We also investigated the upstream signaling of XRASGRP2 in endothelium formation. XRASGRP2 expression was up-regulated in the presence of VEGF-A and down-regulated following VEGF-A depletion. XRASGRP2 knockdown abolished the ectopic induction of endothelial cells by VEGF-A in the posterior ventral blood island. These results suggest that XRASGRP2 is essential for vascular formation during Xenopus development.

PubMed ID: 19598105
Article link: Int J Dev Biol


Species referenced: Xenopus
Genes referenced: aplnr cfd gal.2 hba3 kdr rasgrp2 tek vegfa
Morpholinos: rasgrp2 MO1 rasgrp2 MO2


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