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Dev Dyn November 1, 2009; 238 (11): 2867-76.

Xenopus Rnd1 and Rnd3 GTP-binding proteins are expressed under the control of segmentation clock and required for somite formation.

Goda T , Takagi C , Ueno N .

The process of segmentation in vertebrates is described by a clock and wavefront model consisting of a Notch signal and an fibroblast growth factor-8 (FGF8) gradient, respectively. To further investigate the segmentation process, we screened gene expression profiles for downstream targets of the segmentation clock. The Rnd1 and Rnd3 GTP-binding proteins comprise a subgroup of the Rho GTPase family that show a specific expression pattern similar to the Notch signal component ESR5, suggesting an association between Rnd1/3 and the segmentation clock. Rnd1/3 expression patterns are disrupted by overexpression of dominant-negative or active forms of Notch signaling genes, and responds to the FGF inhibitor SU5402 by a posterior shift analogous to other segmentation-related genes, suggesting that Rnd1/3 expressions are regulated by the segmentation clock machinery. We also show that antisense morpholino oligonucleotides to Rnd1/3 inhibit somite segmentation and differentiation in Xenopus embryos. These results suggest that Rnd1/3 are required for Xenopus somitogenesis.

PubMed ID: 19795516
Article link: Dev Dyn

Species referenced: Xenopus laevis
Genes referenced: actl6a cdc42 clock elob esr-5 gal.2 msgn1 myod1 notch1 odc1 pcdh8 pcdh8.2 rac1 rho rho.2 rhoa rnd1 rnd3 thy1
Antibodies: Somite Ab1
Morpholinos: rnd1 MO1 rnd3 MO1

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