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XB-ART-40418
Proc Natl Acad Sci U S A August 25, 2009; 106 (34): 14426-31.
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Mouse prickle1, the homolog of a PCP gene, is essential for epiblast apical-basal polarity.

Tao H , Suzuki M , Kiyonari H , Abe T , Sasaoka T , Ueno N .


Abstract
Planar cell polarity (PCP) genes are essential for establishing planar cell polarity in both invertebrate and vertebrate tissues and are known to regulate cellular morphogenesis and cell movements during development. We focused on Prickle, one of the core components of the PCP pathway, and deleted one of two mouse prickle homologous genes, mpk1. We found that the deletion of mpk1 gene resulted in early embryonic lethality, between embryonic day (E)5.5 and E6.5, associated with failure of distal visceral endoderm migration and primitive streak formation. The mpk1(-/-) epiblast tissue was disorganized, and analyses at the cellular level revealed abnormal cell shapes, mislocalized extracellular matrix (ECM) proteins, and disrupted orientation of mitotic spindles, from which loss of apico-basal (AB) polarity of epiblast cells are suspected. Furthermore, we show mpk1 genetically interacts with another core PCP gene Vangl2/stbm in the epiblast formation, suggesting that PCP components are commonly required for the establishment and/or the maintenance of epiblast AB polarity. This was further supported by our finding that overexpression of DeltaPET/LIM (DeltaP/L), a dominant-negative Pk construct, in Xenopus embryo disrupted uniform localization of an apical marker PKCzeta, and expanded the apical domain of ectoderm cells. Our results demonstrate a role for mpk1 in AB polarity formation rather than expected role as a PCP gene.

PubMed ID: 19706528
PMC ID: PMC2732806
Article link: Proc Natl Acad Sci U S A


Species referenced: Xenopus
Genes referenced: bmp4 dkk1 hhex mapk1 nodal nodal1 pklr pkm prickle1 prkcz prok1 tjp1 vangl2 wnt3
Antibodies: Itgb1 Ab1 Prkcz Ab1


Article Images: [+] show captions
References [+] :
Bassuk, A homozygous mutation in human PRICKLE1 causes an autosomal-recessive progressive myoclonus epilepsy-ataxia syndrome. 2008, Pubmed