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J Biomed Biotechnol
2009 Jan 01;2009:452567. doi: 10.1155/2009/452567.
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Dermaseptins and magainins: antimicrobial peptides from frogs' skin-new sources for a promising spermicides microbicides-a mini review.
Zairi A
,
Tangy F
,
Bouassida K
,
Hani K
.
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Sexually transmitted infections (STIs) and human immunodeficiency virus (HIV), the causative agents of acquired immunodeficiency syndrome (AIDS), are two great concerns in the reproductive health of women. Thus, the challenge is to find products with a double activity, on the one hand having antimicrobial/antiviral properties with a role in the reduction of STI, and on the other hand having spermicidal action to be used as a contraceptive. In the absence of an effective microbicide along with the disadvantages of the most commonly used spermicidal contraceptive worldwide, nonoxynol-9, new emphasis has been focused on the development of more potential intravaginal microbicidal agents. Topical microbicides spermicides would ideally provide a female-controlled method of self-protection against HIV as well as preventing pregnancies. Nonoxynol-9, the only recommended microbicide spermicide, damages cervicovaginal epithelium because of its membrane-disruptive properties. Clearly, there is an urgent need to identify new compounds with dual potential microbicidal properties; antimicrobial peptides should be candidates for such investigations. Dermaseptins and magainins are two classes of cationic, amphipathic alpha-helical peptides that have been identified in the skin extracts of frogs Phyllomedusa sauvagei and Xenopus laevis. Regarding their contraceptive activities and their effect against various STI-causing pathogens, we believe that these two peptides are appropriate candidates in the evaluation of newer and safer microbicides spermicides in the future.
Figure 1. Effect of dermaseptin on sperm motility. Motile spermatozoa were preincubated in the presence or absence of increasing concentrations of five dermaseptins derivatives for 20 seconds photomicrographics (a) control and (b) in the presence of dermaseptin; complete sperm immobilization was seen after treatment (aggregate of spermatozoa at EC100 = 25 μg/mL)). Original magnification 100 [44].
Figure 2. Effect of spermicidal dermaseptins on sperm acrosome and flagellar plasma membrane integrity. Motile sperm were preincubated in the presence of spermicide solution (EC100 = 25 μg/mL) of K4S4 and was double labelled with Fluorescein Isothiocyanate-conjugated-Pisum sativum Agglutinin for acrosome labelling (FITC/green) and mouse antitubulin antibody for the flagellum staining (CY3/red). The sperm heads exhibited a “dotted” green fluorescence attesting for the presence of an acrosome. At the flagellar level, we observed a loss of membrane integrity since the anti tubulin antibodies have reached their axonemal microtubule target [44].
Albiol Matanic,
Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus.
2004, Pubmed
Albiol Matanic,
Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus.
2004,
Pubmed
Andrès,
Clinical development of antimicrobial peptides.
2005,
Pubmed
Aranha,
Contraceptive efficacy of antimicrobial peptide Nisin: in vitro and in vivo studies.
2004,
Pubmed
Bals,
Epithelial antimicrobial peptides in host defense against infection.
2000,
Pubmed
Brown,
Cationic host defense (antimicrobial) peptides.
2006,
Pubmed
Caporale,
Chance favors the prepared genome.
1999,
Pubmed
Clara,
Preclinical evaluation of magainin-A as a contraceptive antimicrobial agent.
2004,
Pubmed
Coote,
Inhibitory action of a truncated derivative of the amphibian skin peptide dermaseptin s3 on Saccharomyces cerevisiae.
1998,
Pubmed
D'Cruz,
Gel-microemulsions as vaginal spermicides and intravaginal drug delivery vehicles.
2001,
Pubmed
D'Cruz,
Clinical development of microbicides for the prevention of HIV infection.
2004,
Pubmed
D'Cruz,
Structural requirements for potent human spermicidal activity of dual-function aryl phosphate derivative of bromo-methoxy zidovudine (compound WHI-07).
2000,
Pubmed
Dagan,
In vitro antiplasmodium effects of dermaseptin S4 derivatives.
2002,
Pubmed
Efron,
Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture.
2002,
Pubmed
Egal,
Antiviral effects of synthetic membrane-active peptides on herpes simplex virus, type 1.
1999,
Pubmed
,
Xenbase
Feder,
Structure-activity relationship study of antimicrobial dermaseptin S4 showing the consequences of peptide oligomerization on selective cytotoxicity.
2000,
Pubmed
Giovannini,
Biosynthesis and degradation of peptides derived from Xenopus laevis prohormones.
1987,
Pubmed
,
Xenbase
Gura,
Innate immunity. Ancient system gets new respect.
2001,
Pubmed
Hancock,
Host defence (cationic) peptides: what is their future clinical potential?
1999,
Pubmed
Hernandez,
Functional and structural damage in Leishmania mexicana exposed to the cationic peptide dermaseptin.
1992,
Pubmed
Hillier,
In vitro and in vivo: the story of nonoxynol 9.
2005,
Pubmed
Hultmark,
Drosophila immunity: paths and patterns.
2003,
Pubmed
Jacob,
Potential therapeutic applications of magainins and other antimicrobial agents of animal origin.
1994,
Pubmed
,
Xenbase
Krebs,
Sodium dodecyl sulfate and C31G as microbicidal alternatives to nonoxynol 9: comparative sensitivity of primary human vaginal keratinocytes.
2000,
Pubmed
Krugliak,
Antimalarial activities of dermaseptin S4 derivatives.
2000,
Pubmed
Kustanovich,
Structural requirements for potent versus selective cytotoxicity for antimicrobial dermaseptin S4 derivatives.
2002,
Pubmed
Lacombe,
Peptide secretion in the cutaneous glands of South American tree frog Phyllomedusa bicolor: an ultrastructural study.
2000,
Pubmed
,
Xenbase
Lequin,
Dermaseptin S9, an alpha-helical antimicrobial peptide with a hydrophobic core and cationic termini.
2006,
Pubmed
Levy,
Antimicrobial proteins and peptides of blood: templates for novel antimicrobial agents.
2000,
Pubmed
Lorin,
The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro.
2005,
Pubmed
Mor,
Isolation and structure of novel defensive peptides from frog skin.
1994,
Pubmed
Mor,
The vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms.
1994,
Pubmed
,
Xenbase
Naglik,
Candida albicans secreted aspartyl proteinases in virulence and pathogenesis.
2003,
Pubmed
Navon-Venezia,
Antibacterial properties of dermaseptin S4 derivatives with in vivo activity.
2002,
Pubmed
Ong,
Endogenous antimicrobial peptides and skin infections in atopic dermatitis.
2002,
Pubmed
Paquette,
Safety and clinical effects of topical histatin gels in humans with experimental gingivitis.
2002,
Pubmed
Poynten,
The safety of candidate vaginal microbicides since nonoxynol-9: a systematic review of published studies.
2009,
Pubmed
Qu,
Susceptibility of Neisseria gonorrhoeae to protegrins.
1996,
Pubmed
Reddy,
Spermicidal activity of Magainins: in vitro and in vivo studies.
1996,
Pubmed
Reddy,
Antimicrobial peptides: premises and promises.
2004,
Pubmed
Reddy,
Evaluation of the antifertility effect of magainin-A in rabbits: in vitro and in vivo studies.
2000,
Pubmed
Roddy,
Effect of nonoxynol-9 gel on urogenital gonorrhea and chlamydial infection: a randomized controlled trial.
2002,
Pubmed
Rosenstein,
Effect on normal vaginal flora of three intravaginal microbicidal agents potentially active against human immunodeficiency virus type 1.
1998,
Pubmed
Rydlo,
Antibacterial properties of dermaseptin S4 derivatives under extreme incubation conditions.
2006,
Pubmed
Sai,
Investigations on wound healing by using amphibian skin.
1995,
Pubmed
Severini,
The tachykinin peptide family.
2002,
Pubmed
Simmaco,
Antimicrobial peptides from amphibian skin: what do they tell us?
1998,
Pubmed
Stafford,
Safety study of nonoxynol-9 as a vaginal microbicide: evidence of adverse effects.
1998,
Pubmed
Sutyak,
Spermicidal activity of the safe natural antimicrobial peptide subtilosin.
2008,
Pubmed
Uckun,
Prophylactic contraceptives for HIV/AIDS.
1999,
Pubmed
VanCompernolle,
Antimicrobial peptides from amphibian skin potently inhibit human immunodeficiency virus infection and transfer of virus from dendritic cells to T cells.
2005,
Pubmed
Venev,
[Experimental abortions in mice and guinea pigs caused by Aspergillus fumigatus spores].
1976,
Pubmed
Weber,
'Chemical condoms' for the prevention of HIV infection: evaluation of novel agents against SHIV(89.6PD) in vitro and in vivo.
2001,
Pubmed
Yasin,
Susceptibility of Chlamydia trachomatis to protegrins and defensins.
1996,
Pubmed
Zairi,
Spermicidal activity of dermaseptins.
2005,
Pubmed
Zairi,
Susceptibility of Neisseria gonorrhoeae to antimicrobial peptides from amphibian skin, dermaseptin, and derivatives.
2007,
Pubmed
Zairi,
In vitro spermicidal activity of peptides from amphibian skin: dermaseptin S4 and derivatives.
2008,
Pubmed
Zairi,
In vitro activity of dermaseptin S4 derivatives against genital infections pathogens.
2008,
Pubmed
Zaneveld,
Use of mandelic acid condensation polymer (SAMMA), a new antimicrobial contraceptive agent, for vaginal prophylaxis.
2002,
Pubmed
Zasloff,
Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor.
1987,
Pubmed
,
Xenbase
Zasloff,
Antimicrobial activity of synthetic magainin peptides and several analogues.
1988,
Pubmed
,
Xenbase
Zatuchni,
Postcoital, Vaginal, spermicidal potency of formulations: the Macaca arctoides (stumptailed macaque) as animal model.
1981,
Pubmed
Zhang,
Contribution of human alpha-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor.
2002,
Pubmed