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TMBP200, a XMAP215 homologue of tobacco BY-2 cells, has an essential role in plant mitosis.
Yasuhara H
,
Oe Y
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TMBP200 from tobacco BY-2 cells is a member of the highly conserved family of microtubule-associated proteins that includes Xenopus XMAP215, human TOGp, and Arabidopsis MOR1/GEM1. XMAP215 homologues have an essential role in spindle assembly and function in animals and yeast, but their role in plant mitosis is not fully clarified. Here, we show by immunoblot analysis that TMBP200 levels in synchronously cultured BY-2 cells increased when the cells entered mitosis, thus indicating that TMBP200 plays an important role in mitosis in tobacco. To investigate the role of TMBP200 in mitosis, we employed inducible RNA interference to silence TMBP200 expression in BY-2 cells. The resulting depletion of TMBP200 caused severe defects in bipolar spindle formation and resulted in the appearance of multinucleated cells with variable-sized nuclei. This finding indicates that TMBP200 has an essential role in bipolar spindle formation and function.
Cassimeris,
TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.
2004, Pubmed
Cassimeris,
TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.
2004,
Pubmed
Chan,
The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules.
1999,
Pubmed
Chang-Jie,
Identification and preliminary characterization of a 65 kDa higher-plant microtubule-associated protein.
1993,
Pubmed
Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase
Conte,
TACC1-chTOG-Aurora A protein complex in breast cancer.
2003,
Pubmed
,
Xenbase
Cullen,
mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.
1999,
Pubmed
,
Xenbase
Curtis,
A gateway cloning vector set for high-throughput functional analysis of genes in planta.
2003,
Pubmed
Eleftheriou,
Aberrant cell plate formation in the Arabidopsis thaliana microtubule organization 1 mutant.
2005,
Pubmed
Gard,
MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins.
2004,
Pubmed
,
Xenbase
Gergely,
The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells.
2003,
Pubmed
Hamada,
Characterization of a 200 kDa microtubule-associated protein of tobacco BY-2 cells, a member of the XMAP215/MOR1 family.
2004,
Pubmed
Hamada,
GTP is required for the microtubule catastrophe-inducing activity of MAP200, a tobacco homolog of XMAP215.
2009,
Pubmed
,
Xenbase
Holmfeldt,
Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis.
2004,
Pubmed
Hush,
Quantification of microtubule dynamics in living plant cells using fluorescence redistribution after photobleaching.
1994,
Pubmed
Kawamura,
MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root.
2006,
Pubmed
Kawamura,
MOR1, the Arabidopsis thaliana homologue of Xenopus MAP215, promotes rapid growth and shrinkage, and suppresses the pausing of microtubules in vivo.
2008,
Pubmed
,
Xenbase
Kumagai,
Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells.
2001,
Pubmed
Kurihara,
Live cell imaging reveals plant aurora kinase has dual roles during mitosis.
2008,
Pubmed
Nick,
The same, but different--a bird's-eye view on mitosis.
2011,
Pubmed
Niwa,
Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants.
1999,
Pubmed
Shirasu-Hiza,
Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification.
2003,
Pubmed
,
Xenbase
Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Twell,
MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast.
2002,
Pubmed
Weber,
Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy.
1978,
Pubmed
Whittington,
MOR1 is essential for organizing cortical microtubules in plants.
2001,
Pubmed
Yasuhara,
Inhibition of cell-plate formation by brefeldin A inhibited the depolymerization of microtubules in the central region of the phragmoplast.
2000,
Pubmed
Yasuhara,
TMBP200, a microtubule bundling polypeptide isolated from telophase tobacco BY-2 cells is a MOR1 homologue.
2002,
Pubmed
,
Xenbase
Yoneda,
Origin of cortical microtubules organized at M/G1 interface: recruitment of tubulin from phragmoplast to nascent microtubules.
2003,
Pubmed
