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Gene November 1, 2010; 467 (1-2): 13-24.

Regulation of retinal homeobox gene transcription by cooperative activity among cis-elements.

Martinez-de Luna RI , Moose HE , Kelly LE , Nekkalapudi S , El-Hodiri HM .

The retinal homeobox (Rx/rax) gene is essential for the development of the eye. Rax is among the earliest genes expressed during eye development, beginning in the prospective eye fields in the anterior neural plate. Additionally Rax expression persists in retinal progenitor cells and in differentiated photoreceptors. We have isolated and characterized a 2.8 kb genomic DNA fragment that regulates expression of Rax in the developing and maturing retina. We have discovered and characterized cis-acting elements that function to specifically control spatial and temporal Rax expression during retinal development. We have found that the regulation of Rax2A promoter activity requires cooperative interactions between positive and negative regulatory elements. Further, a highly conserved genomic element containing SOX, OTX, and POU transcription factor binding sites is necessary but not sufficient for promoter activity in retinal progenitor or stem cells. Finally, a putative binding element for forkhead transcription factors is necessary for promoter activity and can cooperate with other cis-acting elements to drive Rax2A promoter activity.

PubMed ID: 20627122
PMC ID: PMC2942993
Article link: Gene
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: foxm1 foxn2 foxn4 nppa otx2 pou1f1 pou3f2 rax rax2 rpe

Article Images: [+] show captions
References [+] :
Ambrosetti, Modulation of the activity of multiple transcriptional activation domains by the DNA binding domains mediates the synergistic action of Sox2 and Oct-3 on the fibroblast growth factor-4 enhancer. 2000, Pubmed