Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Muscle Res Cell Motil
2003 Jan 01;247:387-97. doi: 10.1023/a:1027309822007.
Show Gene links
Show Anatomy links
Formation of multiple complexes between beta-dystroglycan and dystrophin family products.
Royuela M
,
Chazalette D
,
Hugon G
,
Paniagua R
,
Guerlavais V
,
Fehrentz JA
,
Martinez J
,
Labbe JP
,
Rivier F
,
Mornet D
.
???displayArticle.abstract???
Beta-dystroglycan is expressed in a wide variety of tissues and has generally been reported with an Mr of 43 kDa, sometimes accompanied with a 31 kDa protein assumed to be a truncated product. This molecule was recently identified as the anomalous beta-dystroglycan expressed in various carcinoma cell lines. We produced and characterized a G5 polyclonal antibody specific to beta-dystroglycan that is directed against the C-terminal portion of the molecule. We provide evidence that beta-dystroglycan may vary in size and properties by studying different Xenopus tissues. Besides normal beta-dystroglycan with an Mr of 43 kDa in smooth and cardiac muscle and sciatic nerve extracts, we found it in skeletal muscle and brain proteins with an Mr of 38 and 65 kDa, respectively. Glycosylation properties and proteolytic susceptibilities of these different beta-dystroglycans are analysed and compared in this work. Crosslinking experiments with various beta-dystroglycan preparations obtained from skeletal and cardiac muscles and brain gave rise to specific new covalent products with Mr of 125 kDa (doublet band), or 120 and 130 kDa, or 140 and 240 kDa, respectively. We provide evidence, using various similar beta-dystroglycan preparations, that the immunoprecipitation procedure with G5 specific polyclonal antibody allows consistent pelleting of various dystrophin-family isoforms. Skeletal muscles from Xenopus reveals the presence of two distinct beta-dystroglycan complexes, one with dystrophin and another one which involves alpha-dystrobrevin. Cardiac muscle and brain from Xenopus are shown to contain three beta-dystroglycan complexes related to various dystrophin-family isoforms. Dystrophin or alpha-dystrobrevin or Dp71 were found in cardiac muscle and dystrophin or Dp180 or Up71 in brain. This variability in the relationship between beta-dystroglycan and dystrophin-family isoforms suggests that each protein--currently known as dystrophin associated protein--could not be present in each of these complexes.
Araishi,
Loss of the sarcoglycan complex and sarcospan leads to muscular dystrophy in beta-sarcoglycan-deficient mice.
1999, Pubmed
Araishi,
Loss of the sarcoglycan complex and sarcospan leads to muscular dystrophy in beta-sarcoglycan-deficient mice.
1999,
Pubmed
Augier,
Proteolytic susceptibility of the central domain in chicken gizzard and skeletal muscle dystrophins.
1992,
Pubmed
Blake,
Coiled-coil regions in the carboxy-terminal domains of dystrophin and related proteins: potentials for protein-protein interactions.
1995,
Pubmed
Blake,
Characterization of a 4.8kb transcript from the Duchenne muscular dystrophy locus expressed in Schwannoma cells.
1992,
Pubmed
Blake,
beta-dystrobrevin, a member of the dystrophin-related protein family.
1998,
Pubmed
Blake,
Dystrobrevin dynamics in muscle-cell signalling: a possible target for therapeutic intervention in Duchenne muscular dystrophy?
2002,
Pubmed
Blake,
Isoform diversity of dystrobrevin, the murine 87-kDa postsynaptic protein.
1996,
Pubmed
Chaussepied,
Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.
1986,
Pubmed
Crawford,
Assembly of the dystrophin-associated protein complex does not require the dystrophin COOH-terminal domain.
2000,
Pubmed
Côté,
Dystroglycan is not required for localization of dystrophin, syntrophin, and neuronal nitric-oxide synthase at the sarcolemma but regulates integrin alpha 7B expression and caveolin-3 distribution.
2002,
Pubmed
Durbeej,
Distribution of dystroglycan in normal adult mouse tissues.
1998,
Pubmed
Elzinga,
F-actin is intermolecularly crosslinked by N,N'-p-phenylenedimaleimide through lysine-191 and cysteine-374.
1984,
Pubmed
Ervasti,
Tissue-specific heterogeneity in alpha-dystroglycan sialoglycosylation. Skeletal muscle alpha-dystroglycan is a latent receptor for Vicia villosa agglutinin b4 masked by sialic acid modification.
1997,
Pubmed
Fabbrizio,
Characterization and localization of a 77 kDa protein related to the dystrophin gene family.
1994,
Pubmed
Fabbrizio,
Dystrophin and dystrophin-related protein expression in Torpedo marmorata electric organ.
1993,
Pubmed
Gieseler,
Overexpression of dystrobrevin delays locomotion defects and muscle degeneration in a dystrophin-deficient Caenorhabditis elegans.
2002,
Pubmed
Gorecki,
Expression of the dystrophin gene in mouse and rat brain.
1991,
Pubmed
Górecki,
Specific expression of G-dystrophin (Dp71) in the brain.
1995,
Pubmed
Górecki,
Expression of four alternative dystrophin transcripts in brain regions regulated by different promoters.
1992,
Pubmed
Górecki,
Dystroglycan: brain localisation and chromosome mapping in the mouse.
1994,
Pubmed
Huang,
Structure of a WW domain containing fragment of dystrophin in complex with beta-dystroglycan.
2000,
Pubmed
Ibraghimov-Beskrovnaya,
Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization.
1993,
Pubmed
Ibraghimov-Beskrovnaya,
Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix.
1992,
Pubmed
Ilsley,
The interaction of dystrophin with beta-dystroglycan is regulated by tyrosine phosphorylation.
2001,
Pubmed
James,
Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin.
2000,
Pubmed
Jung,
Identification and characterization of the dystrophin anchoring site on beta-dystroglycan.
1995,
Pubmed
Koenig,
Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals.
1987,
Pubmed
Lederfein,
A 71-kilodalton protein is a major product of the Duchenne muscular dystrophy gene in brain and other nonmuscle tissues.
1992,
Pubmed
Leschziner,
Neural regulation of alpha-dystroglycan biosynthesis and glycosylation in skeletal muscle.
2000,
Pubmed
Lidov,
Localization of dystrophin to postsynaptic regions of central nervous system cortical neurons.
,
Pubmed
Lidov,
The distribution of dystrophin in the murine central nervous system: an immunocytochemical study.
1993,
Pubmed
Lidov,
Dystrophin in the nervous system.
1996,
Pubmed
Lidov,
Dp140: a novel 140 kDa CNS transcript from the dystrophin locus.
1995,
Pubmed
Losasso,
Anomalous dystroglycan in carcinoma cell lines.
2000,
Pubmed
Matsumura,
Association of dystrophin-related protein with dystrophin-associated proteins in mdx mouse muscle.
1992,
Pubmed
McDearmon,
Differential Vicia villosa agglutinin reactivity identifies three distinct dystroglycan complexes in skeletal muscle.
2001,
Pubmed
Moukhles,
Dystroglycan contributes to the formation of multiple dystrophin-like complexes in brain.
2001,
Pubmed
Nawrotzki,
Characterisation of alpha-dystrobrevin in muscle.
1998,
Pubmed
Pereboev,
Epitopes in the interacting regions of beta-dystroglycan (PPxY motif) and dystrophin (WW domain).
2001,
Pubmed
Pons,
Utrophin localization in normal and dystrophin-deficient heart.
1994,
Pubmed
Rivier,
Different utrophin and dystrophin properties related to their vascular smooth muscle distributions.
1997,
Pubmed
Royuela,
Dystrophin-associated proteins in obliquely striated muscle of the leech Pontobdella muricata (Annelida, Hirudinea).
2001,
Pubmed
Royuela,
Variations in dystrophin complex in red and white caudal muscles from Torpedo marmorata.
2001,
Pubmed
Sadoulet-Puccio,
Dystrobrevin and dystrophin: an interaction through coiled-coil motifs.
1997,
Pubmed
Sciandra,
Identification of the beta-dystroglycan binding epitope within the C-terminal region of alpha-dystroglycan.
2001,
Pubmed
Stedman,
The mdx mouse diaphragm reproduces the degenerative changes of Duchenne muscular dystrophy.
1991,
Pubmed
Tian,
Dystroglycan in the cerebellum is a laminin alpha 2-chain binding protein at the glial-vascular interface and is expressed in Purkinje cells.
1996,
Pubmed
Wells,
Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment 1.
1979,
Pubmed
White,
Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1.
1999,
Pubmed
Wilson,
Up71 and up140, two novel transcripts of utrophin that are homologues of short forms of dystrophin.
1999,
Pubmed
Winder,
The complexities of dystroglycan.
2001,
Pubmed
Winder,
The membrane-cytoskeleton interface: the role of dystrophin and utrophin.
1997,
Pubmed
