Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dev Genes Evol
2011 Mar 01;22011-12:315-27. doi: 10.1007/s00427-010-0349-x.
Show Gene links
Show Anatomy links
Knockdown of SPARC leads to decreased cell-cell adhesion and lens cataracts during post-gastrula development in Xenopus laevis.
Huynh MH
,
Zhu SJ
,
Kollara A
,
Brown T
,
Winklbauer R
,
Ringuette M
.
???displayArticle.abstract??? SPARC is a multifunctional matricellular glycoprotein with complex, transient tissue distribution during embryonic development. In Xenopus laevis embryos, zygotic activation of SPARC is first detected during late gastrulation, undergoing rapid changes in its spatiotemporal distribution throughout organogenesis. Injections of anti-sense Xenopus SPARC morpholinos (XSMOs) into 2- and 4-cell embryos led to a dose-dependent dissociation of embryos during neurula and tailbud stages of development. Animal cap explants derived from XSMO-injected embryos also dissociated, resulting in the formation of amorphous ciliated microspheres. At low doses of XSMOs, lens cataracts were formed, phenocopying that observed in Sparc-null mice. At XSMOs concentrations that did not result in a loss of axial tissue integrity, adhesion between myotomes at intersomitic borders was compromised with a reduction in SPARC concentration. The combined data suggest a critical requirement for SPARC during post-gastrula development in Xenopus embryos and that SPARC, directly or indirectly, promotes cell-cell adhesion in vivo.
Atorrasagasti,
Overexpression of SPARC obliterates the in vivo tumorigenicity of human hepatocellular carcinoma cells.
2010, Pubmed
Atorrasagasti,
Overexpression of SPARC obliterates the in vivo tumorigenicity of human hepatocellular carcinoma cells.
2010,
Pubmed
Barker,
SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity.
2005,
Pubmed
Bradshaw,
SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury.
2001,
Pubmed
Brekken,
SPARC, a matricellular protein: at the crossroads of cell-matrix communication.
2001,
Pubmed
Brown,
Effects of hyaluronan and SPARC on fibroproliferative events assessed in an in vitro bladder acellular matrix model.
2006,
Pubmed
Cardellini,
Tight junctions in early amphibian development: detection of junctional cingulin from the 2-cell stage and its localization at the boundary of distinct membrane domains in dividing blastomeres in low calcium.
1996,
Pubmed
,
Xenbase
Clark,
A prototypic matricellular protein in the tumor microenvironment--where there's SPARC, there's fire.
2008,
Pubmed
Damjanovski,
Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.
1992,
Pubmed
,
Xenbase
Damjanovski,
Ectopic expression of SPARC in Xenopus embryos interferes with tissue morphogenesis: identification of a bioactive sequence in the C-terminal EF hand.
1997,
Pubmed
,
Xenbase
Damjanovski,
Transient expression of SPARC in the dorsal axis of early Xenopus embryos: correlation with calcium-dependent adhesion and electrical coupling.
1994,
Pubmed
,
Xenbase
Dawid,
Mesoderm induction.
1991,
Pubmed
,
Xenbase
Gilmour,
Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.
1998,
Pubmed
Giudici,
Mapping of SPARC/BM-40/osteonectin-binding sites on fibrillar collagens.
2008,
Pubmed
Greiling,
Absence of SPARC leads to impaired lens circulation.
2009,
Pubmed
Hayes,
The distribution of intercellular junctions in the developing myotomes of the clawed toad.
1975,
Pubmed
,
Xenbase
Heasman,
Morpholino oligos: making sense of antisense?
2002,
Pubmed
,
Xenbase
Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase
Henry,
Characterizing gene expression during lens formation in Xenopus laevis: evaluating the model for embryonic lens induction.
2002,
Pubmed
,
Xenbase
Hens,
Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis.
1995,
Pubmed
,
Xenbase
Hohenester,
Structural basis of sequence-specific collagen recognition by SPARC.
2008,
Pubmed
Huynh,
Interaction between SPARC and tubulin in Xenopus.
2004,
Pubmed
,
Xenbase
Kang,
Sparc protein is required for normal growth of zebrafish otoliths.
2008,
Pubmed
Kawasaki,
Gene duplication and the evolution of vertebrate skeletal mineralization.
2007,
Pubmed
Kawasaki,
Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium-binding phosphoprotein family.
2006,
Pubmed
Koehler,
Molecular evolution of SPARC: absence of the acidic module and expression in the endoderm of the starlet sea anemone, Nematostella vectensis.
2009,
Pubmed
Kragtorp,
Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
2006,
Pubmed
,
Xenbase
Lane,
The biology of SPARC, a protein that modulates cell-matrix interactions.
1994,
Pubmed
Martinek,
Is SPARC an evolutionarily conserved collagen chaperone?
2007,
Pubmed
Mason,
Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.
1986,
Pubmed
McClung,
SPARC upregulates MT1-MMP expression, MMP-2 activation, and the secretion and cleavage of galectin-3 in U87MG glioma cells.
2007,
Pubmed
Motamed,
Fibroblast growth factor receptor-1 mediates the inhibition of endothelial cell proliferation and the promotion of skeletal myoblast differentiation by SPARC: a role for protein kinase A.
2003,
Pubmed
Motamed,
Inhibition of PDGF-stimulated and matrix-mediated proliferation of human vascular smooth muscle cells by SPARC is independent of changes in cell shape or cyclin-dependent kinase inhibitors.
2002,
Pubmed
Murphy-Ullrich,
The de-adhesive activity of matricellular proteins: is intermediate cell adhesion an adaptive state?
2001,
Pubmed
Nie,
IFATS collection: Combinatorial peptides identify alpha5beta1 integrin as a receptor for the matricellular protein SPARC on adipose stromal cells.
2008,
Pubmed
Nie,
SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling.
2009,
Pubmed
Norose,
Lenses of SPARC-null mice exhibit an abnormal cell surface-basement membrane interface.
2000,
Pubmed
Norose,
SPARC deficiency leads to early-onset cataractogenesis.
1998,
Pubmed
Nutt,
Comparison of morpholino based translational inhibition during the development of Xenopus laevis and Xenopus tropicalis.
2001,
Pubmed
,
Xenbase
Pottgiesser,
Changes in calcium and collagen IV binding caused by mutations in the EF hand and other domains of extracellular matrix protein BM-40 (SPARC, osteonectin).
1994,
Pubmed
Purcell,
Developmental anomalies of Xenopus embryos following microinjection of SPARC antibodies.
1993,
Pubmed
,
Xenbase
Raines,
The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and -BB and inhibits the binding of PDGF to its receptors.
1992,
Pubmed
Rentz,
SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts.
2007,
Pubmed
Rotllant,
Sparc (Osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear.
2008,
Pubmed
Sage,
Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice.
1989,
Pubmed
Said,
Secreted protein acidic and rich in cysteine (SPARC) inhibits integrin-mediated adhesion and growth factor-dependent survival signaling in ovarian cancer.
2007,
Pubmed
Weaver,
The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase.
2008,
Pubmed
Winklbauer,
Conditions for fibronectin fibril formation in the early Xenopus embryo.
1998,
Pubmed
,
Xenbase
Yan,
Alterations in the lens capsule contribute to cataractogenesis in SPARC-null mice.
2002,
Pubmed
Yan,
SPARC, a matricellular glycoprotein with important biological functions.
1999,
Pubmed
